Czech J. Food Sci., 2022, 40(6):422-426 | DOI: 10.17221/98/2022-CJFS

Effects of washing procedures in reducing Listeria monocytogenes on raw leafy vegetablesOriginal Paper

Nguyen Thi Huong Giang1, Mahmud Ab Rashid Nor-Khaizura*,2,3, Nor Ainy Mahyudin4, Thi Lam An Vu5
1 Faculty of Food Science and Technology, Nong Lam University, Ho Chi Minh City, Vietnam
2 Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Malaysia
3 Laboratory of Food Safety and Food Integrity, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, Serdang, Malaysia
4 Department of Food Service and Management, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Malaysia
5 Department of Food Microbiology, Faculty of Food Science and Technology, Nong Lam University, Ho Chi Minh City, Vietnam

The efficacy of potable water and antimicrobial agents, including turmeric extract, black pepper extract, sodium chloride and sodium bicarbonate, in reducing Listeria monocytogenes on the leaf mustard (Brassica integrifolia) and iceberg lettuce (Lactuca sativa L.) was compared. The uncontaminated samples of two raw leafy vegetables were inoculated with L. monocytogenes at a concentration of 5 log and 7 log colony-forming units (CFU) g-1 for 1 h. At the high L. monocytogenes contamination level, the treatment with black pepper extract 0.6 mg mL-1 for 5 min was found to produce the most considerable decrease in L. monocytogenes counts, resulting in 1.44 log and 1.68 log reduction on leaf mustard and iceberg lettuce, respectively. Similarly, the black pepper extract also showed the highest L. monocytogenes reduction, approximately 0.79 log CFU g-1 on two leafy vegetables at the low contamination level. However, the odour of black pepper extract was left on the vegetables after being washed.

Keywords: foodborne pathogen; leaf mustard; iceberg lettuce; black pepper extract; turmeric extract

Received: June 1, 2022; Accepted: October 31, 2022; Prepublished online: November 24, 2022; Published: November 22, 2022  Show citation

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Thi Huong Giang N, Ab Rashid Nor-Khaizura M, Ainy Mahyudin N, An Vu TL. Effects of washing procedures in reducing Listeria monocytogenes on raw leafy vegetables. Czech J. Food Sci. 2022;40(6):422-426. doi: 10.17221/98/2022-CJFS.
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