Czech J. Food Sci., X:X | DOI: 10.17221/177/2025-CJFS

Optimisation of konjac glucomannan extraction conditions using response surface methodologyOriginal Paper

Jiajia Dai1,2, Yuyan Zhou2,3, Yike Han3, Banan Wei1, Jing Ou1, Xinyue Zhou3, Yujuan Chen1, Shushu Ding1, Guodong Wang ORCID...2,3, Donglin Zhang4
1 School of Public Health, Wannan Medical College, Wuhu, P.R. China
2 Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Wuhu, P.R. China
3 School of Pharmacy, Wannan Medical College, Wuhu, P.R. China
4 School of Stomatology, Wannan Medical College, Wuhu, P.R. China

Konjac glucomannan (KGM), derived from konjac tubers, is a novel functional plant polysaccharide that plays a significant role in antioxidant activity, anti-tumour effects and blood glucose regulation. This study aimed to develop an optimal extraction method for KGM. The extraction yield of KGM was surveyed quantitatively analysed using a response surface central composite design (CCD) to maximise the yield. A quadratic regression model was created to illustrate the interactions between the factors influencing KGM extraction yield. The findings indicated that the effects of extraction temperature and the liquid-to-solid ratio had a greater impact on the extraction yield of KGM than the extraction time and extraction frequency. The optimal extraction conditions for KGM were determined to be an extraction temperature of 100 °C, an extraction time of 2 h, an extraction frequency of 2, and a liquid-to-solid ratio of 113 : 1. Under these optimal conditions, the extraction yield of KGM reached 15.31%.

Keywords: extraction rate; extraction frequency; liquid-solid ratio; extraction temperature; extraction yield

Received: October 21, 2025; Revised: July 2, 2026; Accepted: July 9, 2026; Prepublished online: August 10, 2026 

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