Czech J. Food Sci., 2025, 43(5):365-375 | DOI: 10.17221/100/2025-CJFS

Effect of microbial transglutaminase and banana peel powder on the structure and oxidative potentiality of camel milk yogurt during cold storageOriginal Paper

Rehab S. Alsulami, Elfadil E. Babiker, Isam A. Mohamed Ahmed, Tawfiq S. Alsulami, Fahad Y. Al-Juhaimi
Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia

The current investigation was done to study the impact of adding banana peel powder (BPP) and treatment with microbial transglutaminase (MTGase) on the structure, physicochemical, antioxidant, and sensory qualities of yoghurt prepared from camel milk powder during cold storage. BPP analysis showed that it contained high amounts of total phenolics, which were accompanied by strong antioxidant activity. When employed alone or with BPP, MTGase polymerises yoghurt proteins, forming a structure with a high molecular weight above the stacking gel and lacking pores. The levels of protein, total phenolic compounds (TPC), total solids, ash, and antioxidant activity (DPPH – 2,2-diphenyl-1-picrylhydrazyl) increased after the addition of BPP to yoghurt, even after 21 days of storage. Adding BPP to yoghurt initially decreased thiobarbituric acid reactive substances (TBARS) levels, which then increased with the storage time. Yoghurt made with BPP and treated with MTGase was more stable with enhanced physicochemical, antioxidant, and sensory qualities during storage when compared to yoghurt made with MTGase alone. The results of the study demonstrated that MTGase is capable of cross-linking yoghurt proteins and that BPP can be applied as a stabiliser, antioxidant, and functional component in yoghurt production.

Keywords: transglutaminase; antioxidant; composition; protein; total soluble solids

Received: June 24, 2025; Revised: August 29, 2025; Accepted: September 5, 2025; Published: October 31, 2025  Show citation

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Alsulami RS, Babiker EE, Mohamed Ahmed IA, Alsulami TS, Al-Juhaimi FY. Effect of microbial transglutaminase and banana peel powder on the structure and oxidative potentiality of camel milk yogurt during cold storage. Czech J. Food Sci. 2025;43(5):365-375. doi: 10.17221/100/2025-CJFS.
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    References

    1. Abou-Soliman N.H.I., Sakr S.S., Awad S. (2017): Physico-chemical, microstructural and rheological properties of camel-milk yoghurt as enhanced by microbial transglutaminase. Journal of Food Science and Technology, 54: 1616-1627. Go to original source... Go to PubMed...
    2. Abou-Soliman N.H.I., Awad S., El-Sayed M.I. (2020): The impact of microbial transglutaminase on the quality and antioxidant activity of camel-milk soft cheese. Food and Nutrition Sciences, 11: 153-171. Go to original source...
    3. Ahmad I., Hao M., Li Y., Zhang J., Ding Y., Lyu F. (2022): Fortification of yogurt with bioactive functional foods and ingredients and associated challenges - A review. Trends in Food Science and Technology, 129: 558-580. Go to original source...
    4. Ait El Alia O., Zine-Eddine Y., Kzaiber F., Oussama A., Boutoial K. (2023): Towards the improvement of camel milk consumption in Morocco. Small Ruminant Research, 219: 106888. Go to original source...
    5. Ait El Alia O., Zine-Eddine Y., Chaji S., Boukrouh S., Boutoial K., Faye B. (2025): Global camel milk industry: A comprehensive overview of production, consumption trends, market evolution, and value chain efficiency. Small Ruminant Research, 243: 107441. Go to original source...
    6. Akbari M., Razavi S.H., Kieliszek M. (2021): Recent advances in microbial transglutaminase biosynthesis and its application in the food industry. Trends in Food Science & Technology, 110: 458-469. Go to original source...
    7. Alam M.J., Akter S., Afroze S., Islam M.T., Sayeem E.wH. (2020): Development of fiber and mineral enriched cookies by utilization of banana and banana peel flour. Journal of Microbiology, Biotechnology and Food Sciences, 10: 329-334. Go to original source...
    8. Alenisan M.A., Alqattan H.H., Tolbah L.S., Shori A.B. (2017): Antioxidant properties of dairy products fortified with natural additives: A review. Journal of the Association of Arab Universities for Basic and Applied Sciences, 24: 101-106. Go to original source...
    9. Alia O.A.E., Zine-Eddine Y., Chaji S., Souhassou S., Kzaiber F., Oussama A., Boutoial K. (2023): Physico-chemical and sensory characterization of camel milk yoghurt enriched with persimmon (Diospyros kaki) fruit. Acta Scientiarum Polonorum Technologia Alimentaria, 22: 267-278. Go to original source...
    10. Almusallam I.A., Mohamed Ahmed I.A., Saleh A., Al-Juhaimi F.Y., Ghafoor K., Al Maiman S., Babiker E.E. (2021a): Potential of date palm spikelet extract as an antioxidative agent in set-type yoghurt during cold storage. CyTA - Journal of Food, 19: 190-197. Go to original source...
    11. Almusallam I.A., Ahmed I.A.M., Babiker E.E., Al-Juhaimi F.Y., Saleh A., Qasem A.A., Al Maiman S., Osman M.A., Ghafoor K., Hajji H.A., Al-Shawaker A.S. (2021b): Effect of date palm (Phoenix dactylifera L.) spikelets extract on the physicochemical and microbial properties of set-type yoghurt during cold storage. LWT - Food Science & Technology, 148: 111762. Go to original source...
    12. Al-Zoreky N.S., Al-Otaibi M.M. (2015): Suitability of camel milk for making yogurt. Food Science Biotechnology, 24: 601-606. Go to original source...
    13. Badertscher R., Berger T., Kuhn R. (2007): Densitometric determination of the fat content of milk and milk products. International Dairy Journal, 17: 20-23. Go to original source...
    14. Barros R.F., Cutrim C.S., da Costa M.P., Conte Junior C.A., Cortez M.A.S. (2019): Lactose hydrolysis and organic acids production in yoghurt prepared with different onset temperatures of enzymatic action and fermentation. Ciência Animal Brasileira, 20: e43549. Go to original source...
    15. Benmeziane-Derradji F. (2021): Evaluation of camel milk: Gross composition - A scientific overview. Tropical Animal Health and Production 53: 308. Go to original source... Go to PubMed...
    16. Bulca S., Umut F., Koç A. (2022): The influence of microbial transglutaminase on camel milk yogurt. LWT - Food Science and Technology, 160: 113339. Go to original source...
    17. Bulca S., Büyükgümüş E. (2024): Production of yogurt analogs from peanut milk (extract) using microbial transglutaminase and two different starter cultures. LWT - Food Science and Technology, 205: 116546. Go to original source...
    18. Caipo L., Sandoval A., Sepúlveda B., Fuentes E., Valenzuela R., Metherel A.H., Romero N. (2021): Effect of storage conditions on the quality of arbequina extra virgin olive oil and the impact on the composition of flavor-related compounds (phenols and volatiles). Foods, 10: 2161. Go to original source... Go to PubMed...
    19. Dantas A.B., Jesus V.F., Silva R., Almada C.N., Esmerino E.A., Cappato L.P., Silva M.C., Raices R.S.L., Cavalcanti R.N., Carvalho C.C., Sant'Ana A.S., Bolini H.M.A., Freitas M.Q., Cruz A.G. (2016): Manufacture of probiotic Minas Frescal cheese with Lactobacillus casei Zhang. Journal of Dairy Science, 99: 18-30. Go to original source... Go to PubMed...
    20. Fazilah N.F., Ariff A.B., Khayat M.E., Rios-Solis L., Halim M. (2018): Influence of probiotics, prebiotics, synbiotics and bioactive phytochemicals on the formulation of functional yogurt. Journal of Functional Foods, 48: 387-399. Go to original source...
    21. Feng C., Wang B., Zhao A., Wei L., Shao Y., Wang Y., Cao B., Zhang F. (2019): Quality characteristics and antioxidant activities of goat milk yogurt with added jujube pulp. Food Chemistry, 277: 238-245. Go to original source... Go to PubMed...
    22. Gharibzahedi S.M.T., Chronakis I.S. (2018): Crosslinking of milk proteins by microbial transglutaminase: Utilization in functional yogurt products. Food Chemistry, 245: 620-632. Go to original source... Go to PubMed...
    23. Gomes S., Vieira B., Barbosa C., Pinheiro R. (2022): Evaluation of mature banana peel flour on physical, chemical and texture properties of a gluten-free Rissol. Journal of Food Processing and Preservation, 46: e14441. Go to original source...
    24. Hernández-Carranza P., Ávila-Sosa R., Guerrero-Beltrán J.A., Navarro-Cruz A.R., Corona-Jiménez E., Ochoa-Velasco C.E. (2016): Optimization of antioxidant compounds extraction from fruit by-products: Apple pomace, orange and banana peel. Journal of Food Processing and Preservation, 40: 103-115. Go to original source...
    25. Ho T.M., Zhao J., Bansal N. (2022a): Acid gelation properties of camel milk - Effect of gelatin and processing conditions. Food Bioprocessing Technology, 15: 2363-2373. Go to original source...
    26. Ho T.M., Zou Z., Bansal N. (2022b): Camel milk: A review of its nutritional value, heat stability, and potential food products. Food Research International, 153: 110870. Go to original source... Go to PubMed...
    27. Kamal-Eldin A., Alhammadi A., Gharsallaoui A., Hamed F., Ghnimi S. (2020): Physicochemical, rheological, and micro-structural properties of yoghurts produced from mixtures of camel and bovine milks. NFS Journal, 19: 26-33. Go to original source...
    28. Khalifa M.I., Zakaria A.M. (2019): Physiochemical, sensory characteristics and acceptability of a new set yogurt developed from camel and goat milk mixed with buffalo milk. Advances in Animal and Veterinary Sciences, 7: 172-177. Go to original source...
    29. Khan M.Z., Xiao J., Ma Y., Ma J., Liu S., Khan A., Khan J.M., Cao Z. (2021): Research development on anti-microbial and antioxidant properties of camel milk and its role as an anti-cancer and anti-hepatitis agent. Antioxidants, 10: 788. Go to original source... Go to PubMed...
    30. Kim D.H., Cho W.Y., Yeon S.J., Choi S.H., Lee C.H. (2019): Effects of lotus (Nelumbo nucifera) leaf on quality and antioxidant activity of yogurt during refrigerated storage. Food Science of Animal Resources, 39: 792-803. Go to original source... Go to PubMed...
    31. Laemmli U.K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685. Go to original source... Go to PubMed...
    32. Mohamed Ahmed I.A., Alqah H.A.S., Saleh A., Al-Juhaimi F.Y., Babiker E.E., Ghafoor K., Hassan A.B., Osman M.A., Fickak A. (2021): Physicochemical quality attributes and antioxidant properties of set-type yogurt fortified with Argel (Solenostemma argel Hayne) leaf extract. LWT - Food Science and Technology, 137: 110389. Go to original source...
    33. Mohamed H., Ayyash M., Kamal-Eldin A. (2022): Effect of heat treatments on camel milk proteins - A review. International Dairy Journal, 133: 105404. Go to original source...
    34. Mostafa H.S. (2020): Microbial transglutaminase: An overview of recent applications in food and packaging. Biocatalysis and Biotransformation, 38: 161-177. Go to original source...
    35. Mudgil P., Jumah B., Ahmad M., Hamed F., Maqsood S. (2018): Rheological, micro-structural and sensorial properties of camel milk yogurt as influenced by gelatin. LWT - Food Science and Technology, 98: 646-653. Go to original source...
    36. Pan L.-H., Liu F., Luo S.-Z., Luo J.-P. (2019): Pomegranate juice powder as sugar replacer enhanced quality and function of set yogurts: Structure, rheological property, antioxidant activity and in vitro bioaccessibility. LWT - Food Science and Technology, 115: 108479. Go to original source...
    37. Pascariu O.E., Estevinho L.M., Seixas N.L., Dopcea I., Boiu-Sicuia O.A., Geicu-Cristea M., Israel-Roming F. (2025): Antioxidant properties and microbiological stability of yogurt enriched with elderberry extract. Foods, 14: 1251. Go to original source... Go to PubMed...
    38. Rasidek N.A.M., Nordin M.F.M., Tokuyama H., Nagatsu Y., Mili N., Zaini A.S., Idham Z., Yunus M.A.C. (2021): Subcritical water-based pectin from banana peels (Musa Paradisiaca Cv. Tanduk) as a natural gelation agent. Materials Today: Proceedings, 47: 1329-1335. Go to original source...
    39. Rosmini M.R., Perlo F., Pérez-Alvarez J.A., Pagán-Moreno M.J., Gago-Gago A., López-Santoveña F., Aranda-Catalá V. (1996): TBA test by an extractive method applied to 'paté'. Meat Science, 42: 103-110. Go to original source... Go to PubMed...
    40. Safdari Y., Vazifedoost M., Didar Z., Hajirostamloo B. (2021): The effect of banana fiber and banana peel fiber on the chemical and rheological properties of symbiotic yogurt made from camel milk. International Journal of Food Science, 2021: 5230882. Go to original source... Go to PubMed...
    41. Sidhu J.S., Zafar T.A. (2018): Bioactive compounds in banana fruits and their health benefits. Food Quality and Safety, 2: 183-188. Go to original source...
    42. Swelum A.A., El-Saadony M.T., Abdo M., Ombarak R.A., Hussein E.O.S., Suliman G., Alhimaidi A.R., Ammari A.A., Ba-Awadh H., Taha A.E., El-Tarabily K.A., Abd El-Hack M.E. (2021): Nutritional, anti-microbial and medicinal properties of camel's milk: A review. Saudi Journal of Biological Sciences, 28: 3126-3136. Go to original source... Go to PubMed...
    43. Velazquez-Dominguez A., Hiolle M., Abdallah M., Delaplace G., Peixoto P.P.S. (2023): Transglutaminase cross-linking on dairy proteins: Functionalities, patents, and commercial uses. International Dairy Journal, 143: 105688. Go to original source...
    44. Zaini H.M., Roslan J., Saallah S., Munsu E., Sulaiman N.S., Pindi W. (2022): Banana peels as a bioactive ingredient and its potential application in the food industry. Journal of Functional Foods, 92: 105054. Go to original source...
    45. Zhang Y., Xia Y., Liu X., Xiong Z., Wang S., Zhang N., Ai L. (2019): High-level expression and substrate-binding region modification of a novel BL312 milk-clotting enzyme to enhance the ratio of milk-clotting activity to proteolytic activity. Journal of Agricultural and Food Chemistry, 67: 13684-13693. Go to original source... Go to PubMed...

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