Czech J. Food Sci., 2025, 43(5):358-364 | DOI: 10.17221/93/2025-CJFS

Synbiotic yoghurt with Lactiplantibacillus plantarum and plant powder substratesOriginal Paper

Kristina Veselá ORCID..., Milada Plocková ORCID..., Eva Musilová ORCID..., Blanka Vrchotová ORCID..., ©árka Horáčková ORCID...
Department of Dairy, Fat and Cosmetics, University of Chemistry and Technology, Prague, Czech Republic

Enrichment of yoghurt with probiotic bacterial strains and prebiotic plant substrates has gained increasing interest among both consumers and food manufacturers. In this study, limited growth and fermentation activity of the commercial probiotic strain Lactiplantibacillus plantarum 299v were observed when cultured in ultra-high temperature (UHT) milk. Electron microscopy revealed the strain's ability to adhere to the surface of prebiotically active plant particles, specifically quinoa, lucuma, and baobab powders. Supplementation of these plant substrates at a concentration of 1% (w/v) slightly enhanced the growth of L. plantarum 299v in UHT milk and improved its viability over a 28-day storage period at 5 ± 1 °C. Co-cultivation of L. plantarum 299v with the yoghurt starter culture YC-381 (in a 1 : 0.5 ratio), along with the addition of 1% (w/v) of lucuma, quinoa, or baobab powders to UHT milk, was successfully achieved. These substrates positively influenced the stability of L. plantarum 299v during the 28-day storage and in the case of quinoa of Lactobacillus delbrueckii subsp. bulgaricus during 21-day storage at 5 ± 1 °C. Moreover, the addition of 1% (w/v) plant powders slightly stimulated the production of lactic and acetic acids in yoghurt containing L. plantarum 299v.

Keywords: baobab; quinoa; lucuma; prebiotic; probiotic; stability; viability

Received: June 19, 2025; Revised: July 9, 2025; Accepted: August 5, 2025; Published: October 31, 2025  Show citation

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Veselá K, Plocková M, Musilová E, Vrchotová B, Horáčková ©. Synbiotic yoghurt with Lactiplantibacillus plantarum and plant powder substrates. Czech J. Food Sci. 2025;43(5):358-364. doi: 10.17221/93/2025-CJFS.
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