Czech J. Food Sci., 2024, 42(6):447-455 | DOI: 10.17221/164/2024-CJFS

The deodorising and flavouring effect of enzymatic hydrolysis and glycation on boiled pig trottersOriginal Paper

Dan Qin1, Bulei Sheng2, Shaohong Xu1, Qingyuan Ma1, Zifan Xu1, Min Liu1, Di Zhao3
1 Department of Chemistry, Bengbu Medical University, Bengbu, China
2 College of Tea and Food Science & Technology, Anhui Agriculture University, Anhui, China
3 State Key Laboratory of Meat Quality Control and Cultured Meat Development, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China

Abstract: To eliminate the unpleasant odour and produce more flavour, enzymatic hydrolysis with bromelain (Bro), papain (Pap), and Bro + Pap and boiled with maltose were applied in pig trotters. In this study, Pap, Bro, and a combination of Bro + Pap were dissolved in a saline solution to treat pig trotters. Results showed that the Bro + Pap treatment produced more amino acids, and the boiled + roasted with sugar of Bro + Pap treatment could significantly reduce aldehydes (nonanal and octanal) associated with the formation of off-flavour. Additionally, it increased the content of esters (ethyl propionate, ethyl isobutyrate, ethyl isovalerate, ethyl acetate, methyl acetate, and butyl acetate) in pig trotters, resulting in a more pleasing flavour. Electronic nose signals and sensory evaluation experiments further confirmed these findings. Moreover, the deodorising and aroma-enhancing process also improved their overall eating quality.

Keywords: volatiles; off-flavour; bromelain; papain; electronic nose

Received: August 22, 2024; Revised: November 17, 2024; Accepted: November 27, 2024; Prepublished online: December 13, 2024; Published: December 18, 2024  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Qin D, Sheng B, Xu S, Ma Q, Xu Z, Liu M, Zhao D. The deodorising and flavouring effect of enzymatic hydrolysis and glycation on boiled pig trotters. Czech J. Food Sci. 2024;42(6):447-455. doi: 10.17221/164/2024-CJFS.
Download citation

References

  1. Aaslyng M.D., Meinert L. (2017): Meat flavour in pork and beef - From animal to meal. Meat Science, 132: 112-117. Go to original source... Go to PubMed...
  2. Begum N., Raza A., Song H., Iftikhar M., Zhang Y., Zhang L., Liu P. (2021): Fractionation and identification of flavour peptides from bovine bone extract after enzymatic hydrolysis and Maillard reaction by consecutive chromatography. Journal of Food Processing and Preservation, 45: e15778. Go to original source...
  3. Botinestean C., Gomez C., Nian Y., Auty M.A.E., Kerry J.P., Hamill R.M. (2017): Possibilities for developing texture-modified beef steaks suitable for older consumers using fruit-derived proteolytic enzymes. Journal of Texture Studies, 49: 256-261. Go to original source... Go to PubMed...
  4. Chang Y., Wang S., Chen H., Zhang N., Sun J. (2021): Characterization of the key aroma compounds in pork broth by sensory-directed flavour analysis. Journal of Food Science, 86: 4932-4945. Go to original source... Go to PubMed...
  5. Cheng L., Li X., Tian Y., Wang Q., Li X., An F., Luo Z., Shang P., Liu Z., Huang Q. (2023): Mechanisms of cooking methods on flavour formation of Tibetan pork. Food Chemistry, 19: 10083. Go to original source... Go to PubMed...
  6. Criado C., Chaya C., Fernández-Ruíz V., Álvarez M.D., Herranz B., Pozo-Bayón M.Á. (2019): Effect of saliva composition and flow on inter-individual differences in the temporal perception of retronasal aroma during wine tasting. Food Research International, 126: 108677. Go to original source... Go to PubMed...
  7. Fernández-Lucas J., Castañeda D., Hormigo D. (2017): New trends for a classical enzyme: Papain, a biotechnological success story in the food industry. Trends in Food Science & Technology, 68: 91-101. Go to original source...
  8. Han D., Zhang C.H., Fauconnier M.L., Jia W., Wang J.F., Hu F.F., Xie D.W. (2021): Characterization and comparison of flavour compounds in stewed pork with different processing methods. LWT - Food Science and Technology, 144: 111229. Go to original source...
  9. Kanzler C., Haase P.T. (2020): Melanoidins formed by heterocyclic Maillard reaction intermediates via Aldol reaction and Michael addition. Journal of Agricultural and Food Chemistry, 68: 332-339. Go to original source... Go to PubMed...
  10. Khan M.I., Jo C., Tariq M.R. (2015): Meat flavour precursors and factors influencing flavour precursors - A systematic review. Meat Science, 110: 278-284. Go to original source... Go to PubMed...
  11. Li C., Tu Z., Liu W., Wu C., Hu Y., Wang H. (2023): Flavour substances of low-valued red swamp crayfish (Procambarus clarkii) hydrolysates derived from double enzymatic systems. Food Research International, 165: 112461. Go to original source... Go to PubMed...
  12. Luo J., Yu Q., Han G., Zhang X., Shi H., Cao H. (2022): Identification of off-flavour compounds and deodorizing of cattle by-products. Journal of Food Biochemistry, 46: 111981. Go to original source... Go to PubMed...
  13. Oe M., Wada K., Asikin Y., Arakaki M., Horiuchi M., Takahashi M. (2023): Effects of processing methods on the aroma constituents of hihatsumodoki (Piper retrofractum Vahl). Journal of Food Science, 88: 2463-2477. Go to original source... Go to PubMed...
  14. Penaranda I., Egea M., Linares M.B., López M.B., Garrido M.D. (2024): Marinade injection of pork as a possible technological strategy to reduce boar taint: Response and attitude of the consumer. Meat Science, 212: 109462. Go to original source... Go to PubMed...
  15. Ramalingam V., Song Z., Hwang I. (2019): The potential role of secondary metabolites in modulating the flavour and taste of the meat. Food Research International, 122: 174-182. Go to original source... Go to PubMed...
  16. Shokri S., Ehsani A., Jasour M.S. (2014): Efficacy of lactoperoxidase system-whey protein coating on shelf-life extension of rainbow trout fillets during cold storage (4 °C). Food and Bioprocess Technology, 8: 54-62. Go to original source...
  17. Song S., Li S., Fan L., Hayat K., Xiao Z., Chen L., Tang Q. (2016): A novel method for beef bone protein extraction by lipase-pretreatment and its application in the Maillard reaction. Food Chemistry, 208: 81-88. Go to original source... Go to PubMed...
  18. Wang Y., Song H., Zhang Y., Tang J., Yu D. (2016): Determination of aroma compounds in pork broth produced by different processing methods. Flavour and Fragrance Journal, 31: 319-328. Go to original source...
  19. Xu X., Zheng Y., Song H., Gong L., Pan W. (2018): The effects of enzymatic hydrolysis degree of bovine bone marrow extract on flavour generation via the Maillard reaction. Journal of Food Measurement and Characterization, 13: 521-535. Go to original source...
  20. Xu J.H., Cao H.J., Zhang B., Yao H. (2020): The mechanistic effect of bromelain and papain on tenderization in jumbo squid (Dosidicus gigas) muscle. Food Research International, 131: 108991. Go to original source... Go to PubMed...
  21. Yu X., Chen L., Sheng L., Tong Q. (2016): Volatile compounds analysis and off-flavours removing of porcupine liver. Food Science and Technology Research, 22: 283-289. Go to original source...
  22. Zhang Y., Li H., Zhang Y., Wang L., Zhang P., Jia J., Peng H., Qian Q., Zhang J., Pan Z., Liu D., Zhao L. (2022): Storage stability and flavor change of marinated pork. Foods, 11: 1825. Go to original source... Go to PubMed...
  23. Zhao D., Li H., Huang M., Wang T., Hu Y., Wang L., Xu D., Mao S., Li C., Zhou G. (2020): Influence of proteolytic enzyme treatment on the changes in volatile compounds and odors of beef Longissimus dorsi. Food Chemistry, 333: 127549. Go to original source... Go to PubMed...
  24. Zhou Y., Li B., Wang L. (2021): Effects of different extracts on lipid oxidation and quality characteristics of spiced pork trotter. Journal of Food Processing and Preservation, 46: e16270. Go to original source...
  25. Żołnierczyk A.K., Szumny A. (2021): Sensory and chemical characteristic of two insect species: Tenebrio molitor and Zophobas morio larvae affected by roasting processes. Molecules, 26: 2697. Go to original source... Go to PubMed...

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.