Czech J. Food Sci., 2024, 42(5):372-381 | DOI: 10.17221/49/2024-CJFS

Quality characteristics of Hokkaido brown bear meat sauces prepared with rice koji mold and food enzymesOriginal Paper

Azusa Takeda1, Masataka Saito2, Takeshi Nagai3,4
1 Faculty of Agriculture, Yamagata University, Tsuruoka, Japan
2 Department of Applied Nutrition, Kagawa Nutrition University, Sakado, Japan
3 Graduate School of Agricultural Sciences, Yamagata University, Tsuruoka, Japan
4 The United Graduate School of Agricultural Sciences, Iwate University, Morioka, Japan

Hokkaido brown bear (Ursus arctos yesoensis) meats are used as ingredients of game cuisines. In contrast, shank meats are unsuitable as edible meats due to gamy tastes and tough meats. Here, new meat sauces were developed using glutinous rice koji and food enzymes for the application of the underutilised meat i.e. shank meat. The proximate analysis showed that the obtained sauces were reduced-salt sauces at approximately 6.4–7.7 g·(100 g)–1 as salt equivalent. The sauces had a light colour, no unacceptable odours, and strong sweetness and umami taste. The sauce with good sensory acceptability was rich in glutamic acid, leucine, lysine, and alanine. Besides, the essential amino acid contents were remarkably high at approximately 55.6%. In addition, the tested sauces had good antioxidative activities, scavenging activities against reactive oxygen species such as superoxide anion radicals and hydroxyl radicals, and angiotensin I-converting enzyme and hyaluronidase inhibitory activities. These results suggested that Hokkaido brown bear shank meat sauces, which had positive effects for human health, could be used as one of novel condiment with consumer demands.

Keywords: functionality; game meats; Hokkaido brown bear; meat sauce; underutilised resource

Received: March 14, 2024; Revised: August 21, 2024; Accepted: September 24, 2024; Prepublished online: October 24, 2024; Published: October 30, 2024  Show citation

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Takeda A, Saito M, Nagai T. Quality characteristics of Hokkaido brown bear meat sauces prepared with rice koji mold and food enzymes. Czech J. Food Sci. 2024;42(5):372-381. doi: 10.17221/49/2024-CJFS.
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References

  1. Abe M., Ohnishi S. (2005): Manufacture of fermented meat sauce from silky-fowl. Bulletin of the Kagawa Prefecture Livestock Experiment Station, 40: 28-29.
  2. Aoki M., Komatsu M., Ochiai M., Watanabe F., Igarashi M., Nonomura T., Tomita S., Umehara N., Nakai N., Morishita K. (2010): Healing effects of oral administration of L-citrulline or L-ornithine in a rat pressure-ulcer model-comparison with L-arginine. Japanese Pharmacology and Therapeutics, 38: 807-816.
  3. DiNicolantonio J.J., Lucan S.C. (2014): The wrong white crystals: Not salt but sugar as aetiological in hypertension and cardiometabolic disease. Open Heart, 1: e000167. Go to original source... Go to PubMed...
  4. Freeman M.W., Halvorsen Y-D., Marshall W., Pater M., Isaacsohn J., Pearce C., Murphy B., Nicholas Alp M.P.H., Srivastava A., Bhatt D.L., Brown M.J. (2023): Phase 2 trial of baxdrostat for treatment-resistant hypertension. The New England Journal of Medicine, 388: 395-405. Go to original source... Go to PubMed...
  5. Funatsu Y. (2016): Quality characteristics of Yezo sika deer Cervus nippon yesoensis meat sauce products prepared using different fermentation methods. Journal of the Brewing Society of Japan, 111: 150-159. Go to original source...
  6. Funatsu Y., Miyauchi Y., Kawakami M., Ishioroshi M. (2015): Quality characteristics of Yezo sika deer (Cervus nippon yesoensis) meat sauce products prepared using soy sauce production technology. The Japanese Journal of Zootechnical Science, 86: 53-61. Go to original source...
  7. Gulcin I., Alwasel S.H. (2023): DPPH radical scavenging assay. Processes, 11: 2248. Go to original source...
  8. He F.J., MacGregor G.A. (2010): Reducing population salt intake worldwide: From evidence to implementation. Progress in Cardiovascular Diseases, 52: 363-382. Go to original source... Go to PubMed...
  9. Ito Y., Yamaguchi Y. (2004): Studies on saccharification of mochigome using koji. Journal of Local Independent Administrative Agency Iwate Industrial Research Institute, 11: 11-14.
  10. Japan Soy-sauce Brewers' Association (2023): HACCP Implementation Support Guide (for Beginners). Available at https://www.mhlw.go.jp/content/11130500/000502990.pdf#search=%27%E6%B0%B4%E5%88%86%E6%B4%BB%E6%80%A7%E3%81%A8pH%E3%81%8B%E3%82%89%E8%A6%8B%E3%82%8B%E5%B8%82%E8%B2%A9%E5%93%81%E3%81%97%E3%82%87%E3%81%86%E3%82%86%E3%81%AE%E5%8D%B1%E5%AE%B3%E5%BE%AE%E7%94%9F%E7%89%A9%E3%83%AA%E3%82%B9%E3%82%AF%E3%81%AB%E3%81%A4%E3%81%84%E3%81%A6%27 (accessed Mar 14, 2024; in Japanese).
  11. Japan Soy Sauce Research Institute (1985): Soy Sauce Test. Tokyo, Japan Soy Sauce Research Institute. (in Japanese)
  12. Kagawa A. (2024): Standard Tables of Food Composition in Japan. 8th Ed. Tokyo, Kagawa Nutrition University Publishing Division. (in Japanese)
  13. Kubota K., Morimoto Y. (2021): Food Science-Nutrition Facts and Functionality. 2nd Ed. Tokyo, Tokyo Kagaku Dozin. (in Japanese)
  14. Liem D.G., Miremad F., Keast R.S. (2011): Reducing sodium in foods: The effect on flavor. Nutrients, 3: 694-711. Go to original source... Go to PubMed...
  15. Mikami M., Trang N. H., Shimada K., Sekikawa M., Hukushima M., Ono T. (2007): Properties of 'Shishibishio' fermented pork meat sauce. Nippon Shokuhin Kagaku Kogaku Kaishi, 54: 152-159. Go to original source...
  16. Mills K.T., Bundy J.D., Kelly T.N., Reed J.E., Kearney P.M., Reynolds K., Chen J., He J. (2016): Global disparities of hypertension prevalence and control. Circulation, 134: 441-450. Go to original source... Go to PubMed...
  17. Ministry of Agriculture, Forestry, and Fisheries (2023): Status of Crop Damage Caused by Wild Birds and Animals Nationwide (2022). Available at https://www.maff.go.jp/j/press/nousin/tyozyu/231128.html (accessed Mar 14, 2024; in Japanese).
  18. Murakami Y. (2021): Effects of rice cultivar characteristics on the constituent profile of komeame (rice syrup). Bulletin of the Center for Educational Research and Teacher Development Shizuoka University, 31: 208-218.
  19. Nagai T., Saito M., Tanoue Y., Kai N., Suzuki N. (2020a): Characteristics of low-salt Alaskan pink shrimp sauce prepared using nonglutinous rice cultivar Yukiwakamaru koji. Journal of Food Processing and Preservation, 44: e14747. Go to original source...
  20. Nagai T., Saito M., Tanoue Y., Kai N., Suzuki N. (2020b): Reduced-salt shrimp sauces from Alaskan pink shrimp and nonglutinous rice cultivar Tsuyahime koji: Preparation and characterization. International Aquatic Research, 12: 279-290.
  21. Sekine Y. (2017): Relationship between water activity and pH and inactivation of pathogenic microorganisms. Journal of Soy Sauce Research and Technology, 43: 333-338.
  22. Sun L., Bai Y., Zhang X., Zhou C., Zhang J., Su X., Luo H., Yao B., Wang Y., Tu T. (2021): Characterization of three glutamate decarboxylases from Bacillus spp. for efficient γ-aminobutyric acid production. Microbial Cell Factories, 20: 153. The Brewing Society of Japan (1993): Revised National Tax Administration Agency Analysis Method Commentary. 4th Ed. Tokyo, Commentary Editorial Committee. Go to original source...
  23. Trang N.H., Shimada K., Sekikawa M., Ono T., Mikami M. (2005): Fermentation of meat with koji and commercial enzymes, and properties of its extract. Journal of the Science of Food and Agriculture, 85: 1829-1837. Go to original source...
  24. Tsuchiya K., Nishimura K., Iwahara M. (2003): Purification and characterization of glutamate decarboxylase from Aspergillus oryzae. Food Science and Technology Research, 9: 283-287. Go to original source...
  25. Tsugane S., Sasazuki S., Kobayashi M., Sasaki S. (2004): Salt and salted food intake and subsequent risk of gastric cancer among middle-aged Japanese men and women. British Journal of Cancer, 90: 128-134. Go to original source... Go to PubMed...
  26. Xing L., Lie R., Zhang W., Guanghong Z. (2019): Meat protein based bioactive peptides and their potential functional activity: A review. International Journal of Food Science and Technology, 54: 1956-1966. Go to original source...
  27. Yang J.H., Kawakami M., Ishioroshi M., Funatsu Y. (2012): The quality of meat sauce prepared from culled hens meats. New Food Industry, 54: 43-49.

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