Czech J. Food Sci., 2024, 42(2):127-135 | DOI: 10.17221/181/2023-CJFS

A frontier approach for the production of enteric soft capsules containing omega-3 fatty acids and probioticsOriginal Paper

Hayoung Kim1, Won Yeong Bang1, Boyoung Choi1, Han Bin Lee1, Jungwoo Yang ORCID...2
1 Ildong Bioscience, Pyeongtaek-si, Gyeonggi-do, Republic of Korea
2 Department of Microbiology, College of Medicine, Dongguk University, Gyeongju, Republic of Korea

The study investigated whether omega-3 fatty acids could promote bacterial growth, acid and bile tolerance, and cell adhesion onto Caco-2 cells to develop a multifunctional enteric soft capsule containing probiotics and omega-3 fatty acids. The probiotic strains used were Enterococcus faecium IDCC 2102 and Bacillus coagulans IDCC 1201. Supplementation with omega-3 fatty acids did not significantly affect the growth and viability of either strain at lower concentrations (i.e. 0.5–1.0 w/w %). In comparison, the adhesion ability to Caco-2 cells of both strains was significantly increased (up to 107.5%). Furthermore, Bacillus coagulans IDCC 1201 [1 × 1010 CFU (colony forming unit) per capsule] contained together with omega-3 fatty acids (600 mg per capsule) in the enteric soft capsules showed a stability of over 95% during 12 months of storage at room temperature, which was similar to that of unencapsulated lyophilised probiotics. Thus, these results indicate that multifunctional food supplements in the form of enteric soft capsules are feasible.

Keywords: Bacillus coagulans; Enterococcus faecium; intestinal adhesion; omega-3 fatty acids; probiotics

Received: November 3, 2023; Revised: March 6, 2024; Accepted: March 20, 2024; Prepublished online: April 23, 2024; Published: April 25, 2024  Show citation

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Kim H, Bang WY, Choi B, Lee HB, Yang J. A frontier approach for the production of enteric soft capsules containing omega-3 fatty acids and probiotics. Czech J. Food Sci. 2024;42(2):127-135. doi: 10.17221/181/2023-CJFS.
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