Czech J. Food Sci., 2021, 39(6):452-459 | DOI: 10.17221/146/2020-CJFS

Extraction optimisation and lipid-lowering activity of Auricularia heimuer polysaccharidesOriginal Paper

Xiang Hui Kong1,2,3,4, Yin Peng Ma1,4, Yu Pan1, Wei Jiang1, Ding Jin Li2, Xi Jun Chen3,4, Guo Li Yang3,4, Zhen Hua Duan2, Jie Chi Zhang1,4,*
1 Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin, China
2 Institute of Food Research, Hezhou University, Hezhou, China
3 Heilongjiang Johnsun Biological Engineering Co., Ltd., Harbin, China
4 Heilongjiang Engineering Technology Research Center of Black Fungus Resource Utilization, Harbin, China

Assessments of molecular weight distribution and activity/efficacy of Auricularia heimuer polysaccharides (AAP) are of substantial significance for its extraction process optimisation. In the present study, single-factor orthogonal test and response surface methodology were employed to optimise extraction conditions of AAP. Furthermore, a rat hyperlipidaemia model was established to compare the lipid-lowering activity of polysaccharides obtained by three extraction methods. Conditions for enzymatic hydrolysis were optimised as pH 5.0, 1% cellulase, 2.5% substrate concentration and enzymolysis time of 1.5 h, leading to an up to 31.8% polysaccharide yield and 89.13% of polysaccharides within the molecular weight range of 5 000 Da to 10 000 Da. The results of animal experiments showed that the lipid-lowering activity of enzymolysis-extracted polysaccharides was significantly higher than that of water- and ultrasonic-extracted ones (P < 0.01). So the present study revealed that enzymatic hydrolysis-extracted polysaccharides showed the strongest hypolipidaemia activity, providing a basis for the development of A. heimuer-based functional foods and drugs.

Keywords: hot water extraction; ultrasonic-assisted; cellulase-assisted; molecular weight; HPLC

Published: December 16, 2021  Show citation

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Kong XH, Ma YP, Pan Y, Jiang W, Li DJ, Chen XJ, et al.. Extraction optimisation and lipid-lowering activity of Auricularia heimuer polysaccharides. Czech J. Food Sci. 2021;39(6):452-459. doi: 10.17221/146/2020-CJFS.
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