Czech J. Food Sci., 2021, 39(6):435-443 | DOI: 10.17221/82/2021-CJFS
The state of water is associated with the viability and acidification capacity of Lactobacilli in frozen sourdoughOriginal Paper
- 1 National Engineering Laboratory for Wheat & Corn Further Processing, Henan University of Technology, Zhengzhou, China
- 2 College of Biological Engineering, Henan University of Technology, Zhengzhou, China
The correlations between water state and the key factors affecting sourdough quality, including cell activity and acidification capacity of lactic acid bacteria (LAB), were established in this study. Results revealed that with the increase of frozen storage time, the cell density (CD), total titratable acidity (TTA), and organic acids content declined, whereas the pH value rose. Further, the freezable water content (FWC) and free water quantity (FWQ) decreased, but the total water loss rate (WLR) and immobilised water quantity (IWQ) increased. The CD showed a highly inversely correlation with WLR, and the pH value was strongly inversely correlated to the FWQ. The confocal laser scanning microscopy (CLSM) observed that the ice crystals had larger volumes during frozen storage. Our data, for the first time, disclosed that the total water content and the FWQ may play a crucial role in maintaining the viability and acidification capacity of LAB in frozen sourdough.
Keywords: lactic acid bacteria; cell density; titratable acidity; freezable water; water mobility; water state
Published: December 16, 2021 Show citation
| ACS | AIP | APA | ASA | Harvard | Chicago | Chicago Notes | IEEE | ISO690 | MLA | NLM | Turabian | Vancouver |
References
- Baier-Schenk A., Handschin S., Conde-Petit B. (2005): Ice in prefermented frozen bread dough - An investigation based on calorimetry and microscopy. Cereal Chemistry, 82: 251-255.
Go to original source... - Castro H.P., Teixeira P.M., Kirby R. (2010): Evidence of membrane damage in Lactobacillus bulgaricus following freeze drying. Journal of Applied Microbiology, 82: 87-94.
Go to original source... - Chavan R.S., Chavan S.R. (2011): Sourdough technology - A traditional way for wholesome foods: A review. Comprehensive Reviews in Food Science & Food Safety, 10: 169-182.
Go to original source... - Chen G., Ohgren C., Langton M., Lustrup K.E., Nydén M., Swenson J. (2013): Impact of long-term frozen storage on the dynamics of water and ice in wheat bread. Journal of Cereal Science, 57: 120-124.
Go to original source... - Chen X., Wu J.H., Li L., Wang S.Y. (2017): The cryoprotective effects of antifreeze peptides from pigskin collagen on texture properties and water mobility of frozen dough subjected to freeze-thaw cycles. European Food Research & Technology, 243: 1149-1156.
Go to original source... - Coda R., Xu Y., Moreno D.S., Mojzita D., Nionelli L., Rizzello C.G., Katina K. (2018): Performance of Leuconostoc citreum FDR241 during wheat flour sourdough type I propagation and transcriptional analysis of exopolysaccharides biosynthesis genes. Food microbiology, 76: 164-172.
Go to original source...
Go to PubMed... - Corsetti A., Settanni L. (2007): Lactobacilli in sourdough fermentation. Food Research International, 40: 539-558.
Go to original source... - De Vuyst L., Harth H., Van Kerrebroeck S., Leroy F. (2016): Yeast diversity of sourdoughs and associated metabolic properties and functionalities. International Journal of Food Microbiology, 239: 26-34.
Go to original source...
Go to PubMed... - De Vuyst L., Kerrebroeck S.V., Leroy F. (2017): Microbial ecology and process technology of sourdough fermentation. Advances in Applied Microbiology, 100: 49-160.
Go to original source...
Go to PubMed... - Ding X.L., Zhang H., Wang L., Qian H.F., Qi X.G., Xiao J.H. (2015): Effect of barley antifreeze protein on thermal properties and water state of dough during freezing and freeze-thaw cycles. Food Hydrocolloids, 47: 32-40.
Go to original source... - Frédéric D., Pierre-André M., Gervais P. (2003): Influence of cooling rate on Saccharomyces cerevisiae destruction during freezing: Unexpected viability at ultra-rapid cooling rates. Cryobiology, 46: 33-42.
Go to original source...
Go to PubMed... - Gobbetti M., Rizzello C.G., Di Cagno R., De Angelis M. (2014): How the sourdough may affect the functional features of leavened baked goods. Food Microbiology, 37: 30-40.
Go to original source...
Go to PubMed... - Han B., Bischof J.C. (2004): Direct cell injury associated with eutectic crystallization during freezing. Cryobiology, 48: 8-21.
Go to original source...
Go to PubMed... - Lattanzi A., Minervini F., Gobbetti M. (2014): Assessment of comparative methods for storing type-I wheat sourdough. LWT - Food Science and Technology, 59: 948-955.
Go to original source... - Lucas I., Stauner B., Jekle M., Becker T. (2018): Staining methods for dough systems - Impact on microstructure and functionality. LWT - Food Science & Technology, 88: 139-145.
Go to original source... - Mantzourani I., Plessas S., Odatzidou M., Alexopoulos A., Galanis A., Bezirtzoglou E., Bekatorou A. (2018): Effect of a novel Lactobacillus paracasei starter on sourdough bread quality. Food Chemistry, 271: 259-265.
Go to original source...
Go to PubMed... - Minervini F., De Angelis M., Di Cagno R., Gobbetti M. (2014): Ecological parameters influencing microbial diversity and stability of traditional sourdough. International Journal of Food Microbiology, 171: 136-146.
Go to original source...
Go to PubMed... - Pang T., Zhang H., Wen L. (2021): Quantitative analysis of a weak correlation between complicated data on the basis of principal component analysis. Journal of Analytical Methods in Chemistry, 2021: 1-12.
Go to original source...
Go to PubMed... - Petzold G., Aguilera J.M. (2009): Ice morphology: Fundamentals and technological applications in foods. Food Biophysics, 4: 378-396.
Go to original source... - Phimolsiripol Y., Siripatrawan U., Tulyathan V., Cleland D.J. (2008): Effects of freezing and temperature fluctuations during frozen storage on frozen dough and bread quality. Journal of Food Engineering, 84: 48-56.
Go to original source... - Reale A., Di Renzo T., Preziuso M., Panfili G., Cipriano L., Messia M.G. (2019): Stabilization of sourdough starter by spray drying technique: New breadmaking perspective. LWT - Food Science & Technology, 99: 468-475.
Go to original source... - Ripari V., Bai Y.P., Ganzle M.G. (2019): Metabolism of phenolic acids in whole wheat and rye malt sourdoughs. Food microbiology, 77: 43-51.
Go to original source...
Go to PubMed... - Sadeghi A., Ebrahimi M., Mortazavi S.A., Abedfar A. (2018): Application of the selected antifungal lab isolate as a protective starter culture in pan whole-wheat sourdough bread. Food Control, 95: 298-307.
Go to original source... - Santivarangkna C., Kulozik U., Foerst P. (2010): Inactivation mechanisms of lactic acid starter cultures preserved by drying processes. Journal of Applied Microbiology, 105: 1-13.
Go to original source...
Go to PubMed... - Valdez G.F.D., Diekmann H. (1993): Freeze-drying conditions of starter cultures for sourdoughs. Cryobiology, 30: 185-190.
Go to original source... - Van Kerrebroeck S., Maes D., De Vuyst L. (2017): Sourdoughs as a function of their species diversity and process conditions, a meta-analysis. Trends in Food Science & Technology, 68: 152-159.
Go to original source... - Weckx S., Van Kerrebroeck S., De Vuyst L. (2018): Omics approaches to understand sourdough fermentation processes. International Journal of Food Microbiology, 302: 90-102.
Go to original source...
Go to PubMed... - Xin C., Nie L.J., Chen H.L., Li J., Li B. (2018): Effect of degree of substitution of carboxymethyl cellulose sodium on the state of water, rheological and baking performance of frozen bread dough. Food Hydrocolloids, 80: 8-14.
Go to original source... - Xuan Y.F., Zhang Y., Zhao Y.Y., Zheng Z., Jiang S.T., Zhong X.Y. (2017): Effect of hydroxypropylmethylcellulose on transition of water status and physicochemical properties of wheat gluten upon frozen storage. Food Hydrocolloids, 63: 35-42.
Go to original source... - Yi J., Kerr W.L., Johnson J.W. (2009): Effects of waxy wheat flour and water on frozen dough and bread properties. Journal of Food Science, 74: 278-284.
Go to original source...
Go to PubMed... - Zhao Z., Mu T.H., Sun H.N. (2019): Microbial characterization of five Chinese traditional sourdoughs by high-throughput sequencing and their impact on the quality of potato steamed bread. Food Chemistry, 274: 710-717.
Go to original source...
Go to PubMed... - Zhu T.W., Zhang X., Li B., Wu H. (2019): Effect of interesterified blend-based fast-frozen special fat on the physical properties and microstructure of frozen dough. Food Chemistry, 272: 76-83.
Go to original source...
Go to PubMed...
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.

