Czech J. Food Sci., 2021, 39(3):208-216 | DOI: 10.17221/63/2021-CJFS

Honey: Determination of volatile compounds, antioxidant and antibacterial activitiesOriginal Paper

Semiramis Karlidag1,2, Merve Keskin ORCID...*,3,4, Sinan Bayram5, Nazli Mayda6, Asli Ozkok7
1 Bee and Bee Products Research and Application Center (MTOAGAM), Malatya Turgut Ozal University, Malatya, Turkey
2 Akcadag Vocational School, Malatya Turgut Ozal University, Malatya, Turkey
3 Vocational School of Health Services, Bilecik Seyh Edebali University, Bilecik, Turkey
4 Biotechnology Application and Research Center, Bilecik Seyh Edebali University, Bilecik, Turkey
5 Vocational School of Health Services, Bayburt University, Bayburt, Turkey
6 Department of Pharmaceutical Botany, Pharmacy Faculty, Trakya University, Edirne, Turkey
7 Bee and Bee Products Research and Application Center (HARUM), Hacettepe University, Ankara, Turkey

Honey is an important functional food for human health and nutrition that is collected by honey bees and stored in the honeycombs. In this study, total phenolic content, antioxidant activity and volatile compounds of 13 different honey samples collected from various districts of Malatya province were investigated. As a result of this study, it was determined that the total phenolic content varied between 8.50 mg GAE 100 g-1 and 73.90 mg GAE 100 g-1 and it was observed that the honey samples were rich in aldehydes, aliphatic acid and esters, alcohols, hydrocarbons, carboxylic acid esters, ketones, terpenes, fatty acids and esters. In addition, the antibacterial effects of honey samples were determined against 18 different pathogenic bacteria using agar well diffusion (AWD) method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). In AWD assay, it was recorded that inhibition zone diameters varied between 9 mm and 14 mm and honey samples were found to have a partial inhibitory effect against selected target pathogens.

Keywords: minimum bactericidal concentration; phenolic compounds; minimum inhibitory concentration; antioxidant activity

Published: June 29, 2021  Show citation

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Karlidag S, Keskin M, Bayram S, Mayda N, Ozkok A. Honey: Determination of volatile compounds, antioxidant and antibacterial activities. Czech J. Food Sci. 2021;39(3):208-216. doi: 10.17221/63/2021-CJFS.
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    References

    1. Al-Mamary M.A. (2002): Antioxidant activity of commonly consumed vegetables in Yemen. Malaysian Journal of Nutrition, 8: 179-189.
    2. Barcarolo R., Centeleghe M., Zanatta P., Conte L.S. (1998): GC-MS coupled with head space sampling with reverse carrier flow in sampling step applied to honey characterization. In: 5th International Symposium of Hyphenated Technique in Chromatography, Bruges, Belgium, Feb 1, 1998: 11-21.
    3. Benzie I.F., Strain J.J. (1999): Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology, 299: 15-27. Go to original source... Go to PubMed...
    4. Bobiº O., Mãrghitaº L.A., Dezmirean D., Gherman B., Chirilã F. (2013): In vitro antibacterial activity of unifloral honeys against honeybee pathogens Paenibacillus larvae and Escherichia coli. Lucrãri Stiinşifice - Seria Zootehnie, 55: 160.
    5. Dezmirean D., Mãrghita, L.A., Fiş N., Chirilã F., Gherman B., Mãrgãoan R., Aurori A., Bobiº O. (2015): Antibacterial effect of heather honey (Calluna vulgaris) against different microorganisms of clinical importance. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca (UASVM), Animal Science and Biotechnologies, 72: 72-77. Go to original source...
    6. Ecem Bayram N., Yüzer M.O., Bayram S. (2019): Melissopalynology analysis, physicochemical properties, multielement content and antimicrobial activity of honey samples collected from Bayburt, Turkey. Uludag Bee Journal, 19: 161-176. Go to original source...
    7. Gürbüz S., Özenirler Ç., Mayda N., Çelemli Ö.G., Özkök A. (2019): Pollen spectrum of some honey samples produced in Siirt-Turkey. Hacettepe Journal of Biology and Chemistry, 47: 295-303. Go to original source...
    8. Huttunen S., Riihinen K., Kauhanen J., Tikkanen Kaukanen C. (2013): Antimicrobial activity of different Finnish monofloral honeys against human pathogenic bacteria. Apmis, 121: 827-834. Go to original source... Go to PubMed...
    9. Kassim M., Achoui M., Mustafa M.R., Mohd M.A., Yusoff K.M. (2010): Ellagic acid, phenolic acids, and flavonoids in Malaysian honey extracts demonstrate in vitro antiinflammatory activity. Nutrition research, 30: 650-659. Go to original source... Go to PubMed...
    10. Keskin ª., Mayda N., Keskin M., Özkök A. (2020): Investigation of Bilecik honeys in terms of melissopalynology and chemical analyses (Bilecik ballarinin melissopalinolojik ve kimyasal özellikleri açisindan değerlendirilmesi). Food (Gida), 45: 275-289. (in Turkish) Go to original source...
    11. Ku¶ P.M., Congiu F., Teper D., Sroka Z., Jerkoviæ I., Tuberoso C.I.G. (2014): Antioxidant activity, color characteristics, total phenol content and general HPLC fingerprints of six Polish unifloral honey types. LWT - Food Science and Technology, 55: 124-130. Go to original source...
    12. Lachman J., Orsák M., Hejtmánková A., Kováøová E. (2010): Evaluation of antioxidant activity and total phenolics of selected Czech honeys. LWT - Food Science and Technology, 43: 52-58. Go to original source...
    13. Mãdaº N.M., Mãrghitaº L.A., Dezmirean D.S., Bonta V., Bobiº O., Fauconnier M.L., Francis F., Haubruge E., Nguyen K.B. (2019): Volatile profile and physico-chemical analysis of acacia honey for geographical origin and nutritional value determination. Foods, 8: 445. Go to original source... Go to PubMed...
    14. Mayda N., Keskin M., Keskin ª., Ozkok A. (2019): Determination of botanic origins, total phenolic and flavonoid contents of bee pollen collected from the Bilecik province (Bilecik ilinden toplanan ari polenlerinin botanik orijinleri ile toplam fenolik ve flavonoid içeriklerinin belirlenmesi). Uludag Bee Journal (Uludağ Aricilik Dergisi), 19: 152-160. (in Turkish) Go to original source...
    15. Mohamed M., Sirajudeen K.N.S., Swamy M., Yaacob M., Sulaiman S. (2010): Studies on the antioxidant properties of Tualang honey of Malaysia. African Journal of Traditional, Complementary and Alternative Medicines, 7: 59-63. Go to original source... Go to PubMed...
    16. Osés S.M., Pascual-Mate A., de la Fuente D., de Pablo A., Fernandez-Muino M.A., Sancho M.T. (2016): Comparison of methods to determine antibacterial activity of honeys against Staphylococcus aureus. Journal of the Royal Netherlands Society for Agricultural Sciences (NJAS) - Wageningen Journal of Life Sciences, 78: 29-33. Go to original source...
    17. Pyrzynska K., Biesaga M. (2009): Analysis of phenolic acids and flavonoids in honey. TrAC - Trends in Analytical Chemistry, 28: 893-902. Go to original source...
    18. Sherlock O., Dolan A., Athman R., Power A., Gethin G., Cowman S., Humphreys H. (2010): Comparison of the antimicrobial activity of Ulmo honey from Chile and Manuka honey against methicillin-resistant Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. BioMed Central (BMC) Complementary and Alternative Medicine, 10: 1-5. Go to original source... Go to PubMed...
    19. Singleton V.L., Rossi J.A. (1965): Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16: 144-158. Go to original source...
    20. Singleton V.L., Orthofer R., Lamuela-Raventós R.M. (1999): Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 299: 152-178. Go to original source...
    21. Sorkun K. (2008): Turkey's Nectar Plants, Pollen and Honey (Türkiye'nin Nektarli Bitkileri, Polenleri ve Ballari). Ankara, Turkey, Palme Yayincilik: 341. (in Turkish)
    22. Tamas-Krumpe O., Bobiș O., Mãrgãoan R., Chirilã F., Lațiu C., Ognean L. (2019): Correlative research regarding the total polyphenolic content, antioxidant and antibacterial activity of three types of Romanian honey. Scientific Papers. Series D. Animal Science, LXII: 282-288. Go to PubMed...

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