Czech J. Food Sci., 2020, 38(5):293-300 | DOI: 10.17221/2/2020-CJFS

Modified taro starch as alternative encapsulant for microencapsulation of Lactobacillus plantarum SU-LS 36Original Paper

Raden Haryo Bimo Setiarto1,2, Harsi Dewantari Kusumaningrum ORCID...*,1, Betty Sri Laksmi Jenie1, Tatik Khusniati2, Sulistiani Sulistiani2
1 Department of Food Science and Technology, Faculty of Agricultural Technology and Engineering, Institut Pertanian Bogor (IPB University), Dramaga, West Java, Indonesia
2 Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI) Jalan Raya Jakarta-Bogor, Cibinong Scienc-e Center, Cibinong, Bogor, West Java, Indonesia

Taro starch was modified and used as an alternative encapsulant for the microencapsulation of Lactobacillus plantarum SU-LS 36 by spray drying. Modification of taro starch was conducted by heat moisture treatment (HMT) and 2 autoclaving-cooling cycles (AC-2C). Microencapsulation of L. plantarum SU-LS 36 by spray dryer was done at constant air inlet (125 °C) and outlet temperature (50 °C), feed flow rate (4 mL min-1), drying air flow rate (20 m3 h-1) and air pressure (0.196 MPa). The modified taro starch AC-2C as an encapsulant material was able to produce round-shaped microcapsules and provided optimal protection during spray drying. The modified taro starch AC-2C is very promising to be used as an encapsulant for L. plantarum SU-LS36 since it showed better production yield (40.19%), high encapsulation efficiency (89.83%), protected the encapsulated bacteria from high temperature (70 °C), and showed the lowest viability decreasing during storage up to 6 weeks at room temperature.

Keywords: 2 autoclaving-cooling cycles; microencapsulation; probiotic; resistant starch; spray drying

Published: October 14, 2021  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Setiarto RHB, Kusumaningrum HD, Jenie BSL, Khusniati T, Sulistiani S. Modified taro starch as alternative encapsulant for microencapsulation of Lactobacillus plantarum SU-LS 36. Czech J. Food Sci. 2020;38(5):293-300. doi: 10.17221/2/2020-CJFS.
Download citation

References

  1. Airul A., Yusof M.S.M., Jamil M.S., Abdullah A., Yusoff S.F.M., Arip M.N.M., Lazim A.M. (2014): Physicochemical characterization of starch extracted from Malaysian wild yam (Dioscorea hispida Dennst.). Emirates Journal Food Agriculture, 26: 652-658. Go to original source...
  2. Anekella K., Orsat V. (2013): Optimization of microencapsulation of probiotics in raspberry juice by spray drying. LWT - Food Science and Technology, 50: 17-24. Go to original source...
  3. Arslan-Tontul S., Erbas M. (2017): Single and double layered microencapsulation of probiotics by spray drying and spray chilling. LWT - Food Science and Technology, 81: 160-169. Go to original source...
  4. Ashwar B.A., Gani A., Gani A., Masoodi F.A. (2018): Production of RS4 from rice starch and its utilization as an encapsulating agent for targeted delivery of probiotics. Food Chemistry, 239: 287-294. Go to original source... Go to PubMed...
  5. Brinques G.B., Ayub M.A.Z. (2011): Effect of microencapsulation on survival of Lactobacillus plantarum in simulated gastrointestinal conditions, refrigeration, and yogurt. Journal Food Engineering, 103: 123-128. Go to original source...
  6. Collins T.J. (2007): ImageJ for Microscopy. BioTechnique, 43: S25-S30. Go to original source... Go to PubMed...
  7. Deka D., Sit N. (2016): Dual modification of taro starch by microwave and other heat moisture treatments. International Journal of Biological Macromolecules, 92: 416-422. Go to original source... Go to PubMed...
  8. Dianawati D., Mishra V., Shah N.P. (2013): Stability of microencapsulated Lactobacillus acidophilus and Lactococcus lactis ssp. cremoris during storage at room temperature at low aw. Food Research International, 50: 259-265. Go to original source...
  9. Dos Santos D.X., Casazza A.A., Aliakbariana B., Bedani R., Saad S.M.I., Perego P. (2019): Improved probiotic survival to in vitro gastrointestinal stress in a mousse containing Lactobacillus acidophilus La-5 microencapsulated with inulin by spray drying. LWT - Food Science and Technology, 99: 404-410. Go to original source...
  10. Dutra-Rosolen M., Bordinia F.W., de Oliveiraa P.D., Conceição F.R., Pohndorf R.C., Fiorentini A.M., da Silva W.P., Pieniz S. (2019): Symbiotic microencapsulation of Lactococcus lactis subsp. lactis R7 using whey and inulin by spray drying. LWT - Food Science and Technology, 115: 1-7. Go to original source...
  11. Etchepare M.A., Raddatz G.C., Flores E.M.M., Zepka L.Q., Jacob-Lopes E., Barin J.S., Grosso C.R.F., Menezes C.R. (2016): Effect of resistant starch and chitosan on survival of Lactobacillus acidophilus microencapsulated with sodium alginate. LWT - Food Science and Technology, 65: 511-517. Go to original source...
  12. Gadhiya D., Patel A., Prajapati J.B. (2015): Current trend and future prospective of functional probiotic milk chocolates and related products - A review. Czech Journal of Food Sciences, 33: 295-301. Go to original source...
  13. Gbassi G.K., Vandamme T., Ennahar S., Marchioni E. (2009): Microencapsulation of Lactobacillus plantarum spp. in an alginate matrix coated with whey proteins. International Journal of Food Microbiology, 129: 103-105. Go to original source... Go to PubMed...
  14. Gong P., Sun J., Lin K., Di W., Zhang L., Han X. (2019): Changes process in the cellular structures and constituents of Lactobacillus bulgaricus sp1.1 during spray drying. LWT - Food Science and Technology, 102: 30-36. Go to original source...
  15. Heidebach T., Forst P., Kulozik U. (2012): Microencapsulation of probiotic cells for food applications. Critical Reviews in Food Science and Technology, 52: 291-311. Go to original source... Go to PubMed...
  16. Lisová I., Horáčková ©., Kováčová R., Rada V., Plocková M. (2013): Emulsion encapsulation of Bifidobacterium animalis subsp. lactis Bb12 with the addition of lecithin. Czech Journal of Food Sciences, 31: 270-274. Go to original source...
  17. Rajam R., Anandharamakrishnan C. (2015): Microencapsulation of Lactobacillus plantarum (MTCC 5422) with fructooligosaccharide as wall material by spray drying. LWT - Food Science and Technology, 60: 773-780. Go to original source...
  18. Setiarto R.H.B., Jenie B.S.L., Faridah D.N., Saskiawan I., Sulistiani. (2018): Effect of lactic acid bacteria fermentation and autoclaving-cooling for resistant starch and prebiotic properties of modified taro flour. International Food Research Journal, 25: 1691-1697.
  19. Simsek S., El S.N. (2015): In vitro starch digestibility, estimated glycemic index and antioxidant potential of taro (Colocasia esculenta L. Schott) corm. Food Chemistry, 168: 257-261. Go to original source... Go to PubMed...
  20. Sulistiani S. (2018): Selection of potential probiotic lactic acid bacteria isolated from palm sap (Borassus flabellifer Linn.) origin Kupang, East Nusa Tenggara. In: AIP Conference Proceedings, 2002, 020059. Go to original source...
  21. Tárrega A., Rocafull A., Costell E. (2010): Effect of blends of short and long-chain inulin on the rheological and sensory properties of prebiotic low-fat custards. LWT - Food Science and Technology, 43: 556-562. Go to original source...
  22. Ying D.Y., Schwanderc S., Weerakkody R., Sanguansri L., Gantenbein-Demarchi C., Augustin M.A. (2013): Microencapsulated Lactobacillus rhamnosus GG in whey protein and resistant starch matrices: Probiotic survival in fruit juice. Journal of Functional Foods, 5: 98-105. Go to original source...

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.