Czech J. Food Sci., 2020, 38(3):137-143 | DOI: 10.17221/308/2019-CJFS

Grapevine extracts and their effect on selected gut-associated microbiota: In vitro studyOriginal Paper

Michaela Rollová1, Lucia Gharwalová1, Ale¹ Krmela2, Vìra Schulzová2, Jana Haj¹lová2, Petr Jaro¹3, Irena Kolouchová*,1, Olga Ma»átková1
1 Department of Biotechnology, University of Chemistry and Technology, Prague, Czech Republic
2 Department of Food Analysis and Nutrition, University of Chemistry and Technology, Prague, Czech Republic
3 Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Czech Republic

The biological activity of polyphenol substances contained in food supplements prepared from Vitis vinifera can affect the microorganisms present in the digestive tract in terms of their representation and activity of the individual species. This study deals with resveratrol and two polyphenol-rich extracts (extract from V. vinifera canes and the commercial product Regrapex-R-forte) and their effect on selected gut microbiota (Bifidobacterium animalis subsp. lactis Bb-12, Lactobacillus acidophilus LA-5, Lactobacillus casei Lafti L-26, Citrobacter freundii DBM 3127, Escherichia coli DBM 3125). The effect of the studied agents on planktonic and biofilm growth of the microorganisms was determined as minimum inhibitory concentration (MIC80) and minimum biofilm inhibitory concentration (MBIC80), respectively. The extracts induced metabolic activity as well as total biofilm biomass production in probiotic strain L. acidophilus LA-5 while successfully inhibiting the growth of opportunistic pathogenic microorganisms C. freundii DBM 3127 and E. coli DBM 3125.

Keywords: anti-biofilm activity; gut microbiota; polyphenols; resveratrol; Vitis vinifera

Published: June 30, 2020  Show citation

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Rollová M, Gharwalová L, Krmela A, Schulzová V, Haj¹lová J, Jaro¹ P, et al.. Grapevine extracts and their effect on selected gut-associated microbiota: In vitro study. Czech J. Food Sci. 2020;38(3):137-143. doi: 10.17221/308/2019-CJFS.
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