Czech J. Food Sci., 2019, 37(4):221-225 | DOI: 10.17221/235/2018-CJFS

Efficacy of chemical agents and power ultrasound on biofilms formed by Asaia spp. - spoilage bacteria in beverage industriesFood Microbiology and Safety

Iveta ©ístková*, Iveta Horsáková, Miroslav Čeřovský, Helena Číľková
Department of Food Preservation, University of Chemistry and Technology Prague, Prague, Czech Republic

Spoilage bacteria were isolated from spoiled fruit-flavoured bottled water with a visual defect in the form of floating flocks. The bacteria were identified as Asaia lannensis using the PCR technique. The efficacy of five chemical agents commonly used in the beverage industry and of power ultrasound (PUS) on Asaia biofilms was studied. A static cultivation procedure on stainless steel plates was used and the efficacy of the chemical agents was tested in two stages. First, only the chemical agents were used. Second, the effect of the application of PUS for 1 min prior to the application of the chemical agents was tested. The most effective chemical agent was the one based on peracetic acid. Its use without PUS proved to be more effective than a combination of any of the other chemical agents with PUS. The least effective methods included the physical sanitation by PUS, the chemical agent based on a 10% solution of sodium hypochlorite and sodium hydroxide, and the chemical agent containing a 0.3% solution of chlorine dioxide.

Keywords: Asaia lannensis; biofilm; cleaning agent; disinfectant; non-alcoholic beverage

Published: August 31, 2019  Show citation

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©ístková I, Horsáková I, Čeřovský M, Číľková H. Efficacy of chemical agents and power ultrasound on biofilms formed by Asaia spp. - spoilage bacteria in beverage industries. Czech J. Food Sci. 2019;37(4):221-225. doi: 10.17221/235/2018-CJFS.
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    References

    1. Belessi Ch.-E.A., Gounadaki A.S., Psomas A.N., Skandamis P.N. (2011): Efficiency of different sanitation methods on Listeria monocytogenes biofilms formed under various environmental conditions. International Journal of Food Microbiology, 145: S46-S52. Go to original source... Go to PubMed...
    2. Erriu M., Blus C., Szmukler-Moncler S., Buogo S., Levi R., Barbato G., Madonnaripa D., Denotti G., Piras V., Orrù G. (2014): Microbial biofilm modulation by ultrasound: Current concepts and controversies. Ultrasonics Sonochemistry, 21: 15-22. Go to original source... Go to PubMed...
    3. Florjanič M., Kristl J. (2011): The control of biofilm formatin by hydrodynamice of purified water in industrial distribution system. International Journal of Pharmaceutics, 405: 16-22. Go to original source... Go to PubMed...
    4. Fratamico P.M., Annous B.A., Gunther N.W. (eds): Biofilm in the Food and Beverage Industries (1st Ed.). Cambridge, Woodhead Publishing: 600
    5. Horsáková I., Voldřich M., Čeřovský M., Sedláčková P., ©icnerová P., Ulbrich P. (2009): Asaia sp. as a bacterium decaying the packaged still fruit beverages. Czech Journal of Food Sciences, 27: S366-S368. Go to original source...
    6. Juvonen R., Virkajärvi V., Priha O., Laitila A. (2011): Microbiological Spoilage and Safety Risks in Noon-beer Beverages Produced in a Brewery Environment. Espoo, VTT Tiedotteita-Research Centre of Finland: 107.
    7. Kitis M. (2004): Disinfection of wastewater with peracetic acid: a review. Environment International, 30: 47-55. Go to original source... Go to PubMed...
    8. Kregiel D., Rygala A., Libudzisz Z., Walczak P., OltuszakWalczak E. (2012): Asaia lannensis - the spoilage acetic acid bacteria isolated from strawberry-flovored bottled water in Poland. Food Control, 26: 147-150. Go to original source...
    9. Kregiel D. (2013): Attachment of Asaia lannensis to materials commonly used in beverage industry. Food Control, 32: 537-542. Go to original source...
    10. Mariscal A., Carnero-Varo M., Gutierrez-Bedmar M., Garcia-Rodriguez A., Fernandez-Crehuet J. (2007): A fluorescent method for assessing the antimicrobial efficacy of disinfectant against Escherichia coli ATCC 35218 biofilm. Applied Microbiology and Biotechnology, 77: 233-240. Go to original source... Go to PubMed...
    11. McDonnell G., Russel A.D. (1999): Antiseptics and disinfectants: activity, action, and resistance. Clinical Microbiology Reviews, 12: 147-179. Go to original source... Go to PubMed...
    12. Moore J.E., McCalmont M., Xu J., Millar B.C., Heaney N. (2002): Asaia sp., an unusual spoilage organism of fruitflavoured bottled water. Applied and Environmental Microbiology, 68: 4130-4131. Go to original source... Go to PubMed...
    13. Purkrtová S., Turoňová H., Pilchová T., Demnerová K., Pazlarová J. (2010): Resistance of Listeria monocytogenes biofilms to disinfectants. Czech Journal of Food Sciences, 28: 326-332. Go to original source...
    14. Sedláčková P., Čeřovský M., Horsáková I., Voldřich M. (2011): Cell surface characteristic of Asaia bogorensis - spoilage microorganism of bottled water. Czech Journal of Food Sciences, 29: 457-461. Go to original source...
    15. Simões M., Simões L.C., Vieira M.J. (2010): A review of current and emergent biofilm control strategies. LWT-Food Science and Technology, 43: 573-583. Go to original source...

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