Czech J. Food Sci., 2018, 36(2):126-132 | DOI: 10.17221/340/2017-CJFS

Determining the optimal method for DNA isolation from fruit jamsFood Microbiology and Safety

Tereza SOVOVÁ*, Barbora KŘÍŽOVÁ, Jaroslava OVESNÁ
Department of Molecular Genetics, Crop Research Institute, Prague, Czech Republic

DNA extraction is a crucial step in PCR analysis especially when analysing food samples that can be degraded and can potentially contain PCR-inhibiting substances. In this study, we compared the suitability of three DNA extraction methods - two kits: DNeasy® Plant Mini Kit and NucleoSpin® Food, and the CTAB method - for DNA extraction from commercial fruit jams. Fourteen jams with different contents of fruit, sugar and other additives were extracted in triplicate using the above-mentioned methods directly and after a washing step. The concentration and optical density were analysed using UV spectrophotometry and the amplifiability of the obtained DNA was evaluated using a PCR assay targeting a sequence coding for chloroplast tRNA-Leu. Samples isolated using the NucleoSpin® Food kit contained non-amplifiable DNA in eight cases, and samples isolated using the CTAB method could not be quantified. The DNeasy® Plant Mini Kit thus proved to be the most suitable method, since well-amplifiable DNA was obtained for all the analysed samples.

Keywords: DNA amplifiability; DNA extraction; food analysis; food quality

Published: April 30, 2018  Show citation

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SOVOVÁ T, KŘÍŽOVÁ B, OVESNÁ J. Determining the optimal method for DNA isolation from fruit jams. Czech J. Food Sci. 2018;36(2):126-132. doi: 10.17221/340/2017-CJFS.
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