Czech J. Food Sci., 2017, 35(5):386-391 | DOI: 10.17221/243/2016-CJFS

Efficiency of PCR-RFLP and species-specific PCR for the identification of meat origin in dry sausagesFood Analysis, Food Quality and Nutrition

Ivona Djurkin Kušec^*,1, Danijela Samac¹, Vladimir Margeta¹, Žarko Radišić¹, Dragutin Vincek², Goran Kušec¹

¹ Faculty of Agriculture, J.J. Strossmayer University of Osijek, Osijek, Croatia

² Department of Agriculture, Varaždin County, Varaždin, Croatia

The purpose of this investigation was the identification of chicken, beef and sheep meat in pork sausages using PCR-RFLP and PCR with pecies-specific primers. Six dry fermented pork sausages were produced by adding beef, sheep and chicken meat to each in the amount of 1 and 5%. DNA was extracted from five regions of each sausage and PCR-RFLP together with PCR using species-specific primers was performed. PCR-RFLP analysis was successful only for chicken meat, while species-specific PCR was effective for identification of chicken, eef and sheep meat in all ratios and from all regions of the sausages. The results of our study show that discovering adulteration using PCR-RFLP is suitable only for chicken meat in the investigated products, while for detection of beef and sheep meat use of species-specific oligonucleotides is more effective.

Keywords: DNA; traceability; meat species; dry fermented product

Published: October 31, 2017  Show citation