Czech J. Food Sci., 2017, 35(5):376-385 | DOI: 10.17221/103/2017-CJFS

Components responsible for antimicrobial activity of propolis from continental and Mediterranean regions in CroatianFood Microbiology and Safety

Ivana Tlak Gajger1, Iva Pavlović2, Mirza Bojić3, Ivan Kosalec3, Siniša Srečec4, Toni Vlainić2, Josipa Vlainić*,2
1 Faculty of Veterinary Medicine and 3Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia
2 Ruđer Bošković Institute, Zagreb, Croatia
4 Križevci College of Agriculture, Križevci, Croatia

Propolis is a popular subject of research worldwide due to its therapeutic potential. The antimicrobial activity of propolis appears to be promising but depends on many variables related to its origin such as the content of phenolics and flavonoids. To address this issue with Croatian propolis, which has two major origins (Mediterranean and continental), we exposed bacteria (Escherichia coli and Staphylococcus aureus) and yeasts (Candida albicans and Aspergillus niger) to different propolis concentrations (two-fold microdilution method with TCC/formazan endpoint). Total phenolic and flavonoid content and chromatographic profile along with antioxidant activity were assessed. The majority of the 24 propolis samples tested exhibited potent antimicrobial activity against S. aureus bacteria and the yeast C. albicans. Most propolis samples also exhibited robust antioxidative capacity which correlated polyphenol and flavonoid content. To the best of our knowledge this is the first study in which the antimicrobial activity of Croatian propolis is correlated with its constituents.

Keywords: propolis; antimicrobial effects; phenols; flavonoids; DPPH; FRAP; HPLC analyses; correlations

Published: October 31, 2017  Show citation

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Tlak Gajger I, Pavlović I, Bojić M, Kosalec I, Srečec S, Vlainić T, Vlainić J. Components responsible for antimicrobial activity of propolis from continental and Mediterranean regions in Croatian. Czech J. Food Sci. 2017;35(5):376-385. doi: 10.17221/103/2017-CJFS.
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    References

    1. Bankova V., Popova M., Trusheva B. (2014): Propolis volatile compounds: chemical diversity and biological activity: a review. Chemistry Central Journal, 8: 28. doi: 10.1186/1752-153X-8-28 Go to original source... Go to PubMed...
    2. Banskota A.H., Tezuka Y., Kadota S. (2001): Recent progress in pharmacological research of propolis. Phytotheraphy Research, 15: 561-571. Go to original source... Go to PubMed...
    3. Barbarić M., Mišković K., Bojić M., Lončar M.B., SmolčićBubalo A., Debeljak Z., Medić-Šarić M. (2011): Chemical composition of the ethanolic propolis extracts and its effect on HeLa cells. Journal of Ethnopharmacology, 135: 772-778. Go to original source... Go to PubMed...
    4. Benzie I.F., Strain J.J. (1999): Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology, 299: 15-27. Go to original source... Go to PubMed...
    5. Bonheví J.S., Gutiérrez A.L. (2012): The antimicrobial effects of propolis collected in different regions in the Basque country (Northern Spain). World Journal of Microbiology and Biotechnology, 28: 1351-1358. Go to original source... Go to PubMed...
    6. Boyanova L., Kolarov R., Gergova G., Mitov I. (2006): In vitro activity of Bulgarian propolis against 94 clinical isolates of anaerobic bacteria. Anaerobe, 12: 173-177. Go to original source... Go to PubMed...
    7. Brand-Williams W., Cuvelier M.E., Berset C. (1995) Use of a free radical method to evaluate antioxidant activity. LWT- Food Science and Technology, 28: 25-30. Go to original source...
    8. Celikel N., Kavas G. (2008): Antimicrobial properties of some essential oils against some pathogenic microorganisms. Czech Journal of Food Sciences, 26: 174-181. Go to original source...
    9. Choi Y.M., Noh D.O., Cho S.Y., Suh H.J., Kim K.M., Kim J.M. (2006): Antioxidant and antimicrobial activities of propolis from several regions of Korea. LWT-Food Science and Technology, 39: 756-761. Go to original source...
    10. da Silva J.F.M., de Souza M.C., Matta S.R., de Andrade M.R., Vidal F.V.N. (2006): Correlation analysis between phenolic levels of Brazilian propolis extracts and their antimicrobial and antioxidant activities. Food Chemistry, 99: 431-435. Go to original source...
    11. D'Auria F.D., Tecca M., Scazzocchio F., Renzini V., Strippoli V. (2003): Effect of propolis on virulence factors of Candida albicans. Journal of Chemotherapy, 15: 454-460. Go to original source... Go to PubMed...
    12. Drago L., Mombelli B., De Vecchi E., Fassina M.C., Tocalli L., Gismondo M.R. (2000): In vitro antimicrobial activity of propolis dry extract. Journal of Chemotheraphy, 12: 390-395. Go to original source... Go to PubMed...
    13. EUCAST Discussion Document E. Def 2003, 5.1. (2003): European Committee on Antimicrobial Susceptibility Testing (EUCAST). Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by broth microdilution. Clinical Microbiology and Infection, 9: 1-10. Available at (http://www.escmid.org/Seviware/Script/SvFiles.asp?Ref=359) Go to PubMed...
    14. Grenho L., Barros J., Ferreira C., Santos V.R., Monteiro F.J., Ferraz M.P., Cortes M.E. (2015): In vitro antimicrobial activity and biocompatibility of propolis containing nanohydroxyapatite. Biomedical Materials, 10 (2): 025004. doi: 10.1088/1748-6041/10/2/025004 Go to original source... Go to PubMed...
    15. Hamasaka T., Kumazawa S., Fujimoto T., Nakayama T. (2004): Antioxidant activity and constituents of propolis collected in various areas of Japan. Food Science and Technology Research, 10: 86-92. Go to original source...
    16. Kalogeropoulos N., Konteles S.J., Troullidou E., Mourtzinos I., Karathanos V. (2009): Chemical composition, antioxidant activity and antimicrobial properties of propolis extract from Greece and Cyprus. Food Chemistry, 116: 452-461. Go to original source...
    17. Kosalec I., Sankovic K., Zovko M., Bakmaz M., Kalogjera Z., Pepljnjak S. (2007): Antimicrobial and antioxidant activity of propolis from Croatia and Brazil: a comparative study. In: Bauer R. (ed.): Planta Medica: Book of abstracts 55th International Congress and Annual Meeting of the Society for Medicinal Plant Research, Sept 2-6, 2007, Graz, Austria. Go to original source...
    18. Kujumgiev A., Tsvetkova I., Serkedjieva Yu., Bankova V., Christov R., Popov S. (1999): Antibacterial, antifungal and antiviral activity of propolis from different geographic origins. Journal of Ethnopharmacology, 64: 235-240. Go to original source... Go to PubMed...
    19. Machado C.S., Mokochinski J.B., de Lira T.O., de Oliveira F.D.C.E., Cardoso M.V., Ferreira R.G., Sawaya A.C., Ferreira A.G., Pessoa C., Cuesta-Rubio O., Monteiro M.C., de Campos M.S., Torres Y.R. (2016): Comparative study of chemical composition and biological activity of yellow, green, brown, and red Brazilian propolis. Evidence-Based Complementary and Alternative Medicine, 2016, Article ID 6057650. doi: 10.1155/2016/6057650 Go to original source... Go to PubMed...
    20. Medić-Sarić M., Rastija V., Bojić M. (2011): Recent advances in the application of high performance liquid chromatography in the analysis of polyphenols in wine and propolis. Journal of AOAC International, 94: 32-42. Go to original source... Go to PubMed...
    21. Moreno M.I.N., Isla M.I., Cudmani N.G., Vattuone M.A., Sampietro A.R. (1999): Screening of antibacterial activity of Amaicha del Valle (Tucumán, Argentina) propolis. Journal of Ethnopharmacology, 68: 97-102. Go to original source... Go to PubMed...
    22. Nina N., Quispe C., Jiménez-Aspee F., Theoduloz C., Feresín G.E., Lima B., Leiva E., Schmeda-Hirschmann G. (2015): Antibacterial activity, antioxidant effect and chemical composition of propolis from the Región del Maule, Central Chile. Molecules, 20: 18144-18167. Go to original source... Go to PubMed...
    23. Popova M., Silici S., Kaftanoglu O., Bankova V. (2005): Antibacterial activity of Turkish propolis and its qualitative and quantitative chemical composition. Phytomedicine, 12: 221-228. Go to original source... Go to PubMed...
    24. Šamec D., Piljac-Žegarac J., Bogović M., Habjanič K., Grúz J. (2011): Antioxidant potency of white (Brassica oleracea L. var. capitata) and Chinese (Brassica rapa L. var. pekinensisi (Lour.)) cabbage: The influence of development stage, cultivar choice and seed selection. Scientia Horticulturae. 128: 78-83. Go to original source...
    25. Sawaya A.C., Palma A.M., Caetano F.M., Marcucci M.C., Cunha I.B.S., Araujo C.E. (2002): Comparative study of in vitro methods used to analyse the activity of propolis extracts with different compositions against species of Candida. Letters in Applied Microbiology, 35: 203-207. Go to original source... Go to PubMed...
    26. Sforcin J.M., Fernandes A. Jr., Lopes C.A., Bankova V., Funari S.R. (2000): Seasonal effect on Brazilian propolis antibacterial activity. Journal of Ethnopharmacology, 73: 243-249. Go to original source... Go to PubMed...
    27. Sforcin J.M., Bankova V. (2011): Propolis: is there a potential for the development of new drugs? Journal of Ethnopharmacology, 133: 253-260. Go to original source... Go to PubMed...
    28. Singleton V.L., Rossi J.A. (1965): Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16: 144-158. Go to original source...
    29. Socha R., Gałkowska D., Bugaj M., Juszczak L. (2015): Phenolic composition and antioxidant activity of propolis from various regions of Poland. National Product Research, 29: 416-422. Go to original source... Go to PubMed...
    30. Trusheva B., Trunkova D., Bankova V. (2007): Different extraction methods of biologically active components from propolis: a preliminary study. Chemistry Central Journal, 1: 13. Go to original source... Go to PubMed...
    31. Vera N., Solorzano E., Ordoñez R., Maldonado L., Bedascarrasbure E., Isla M.I. (2011): Chemical composition of Argentinean propolis collected in extreme regions and its relation with antimicrobial and antioxidant activities. National Product Communications, 6: 823-827. Go to original source...
    32. Wang X., Sankarapandian K., Cheng Y., Woo S.O., Kwon H.W., Perumalsamy H., Ahn Y.J. (2016): Relationship between total phenolic contents and biological properties of propolis from 20 different regions in South Korea. BMC Complementary and Alternative Medicine, 16: 65. doi: 10.1186/s12906-016-1043-y Go to original source... Go to PubMed...
    33. Zhisten J., Mengcheng T., Jiamming W. (1999): The determination of flavonoid contents in mulberry and their scavenging effect on superoxide radicals. Food Chemistry, 64: 555-559. Go to original source...
    34. Živković J., Zeković Z., Mujić I., Vidović S., Cvetković D., Lepojević Ž., Nikolić G., Trutić N. (2010): Scavenging capacity of superoxide radical and screening of antimicrobial activity of Castanea sativa Mill. extracts. Czech Journal of Food Sciences, 28: 61-68. Go to original source...

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