Czech J. Food Sci., 2017, 35(1):89-94 | DOI: 10.17221/82/2016-CJFS

Ultrasonic microwave-assisted extraction of polyphenols, flavonoids, triterpenoids, and vitamin C from Clinacanthus nutansFood Technology and Economy, Engineering and Physical Properties

Qun Yu1, Chuan Li1*, Zhenhua Duan1, 2*, Bing Liu1, Weiwen Duan2, Feifei Shang2
1 College of Food Science, Hainan University, Haikou, P.R. China
2 Institue of Food Research, Hezhou University, Hezhou, P.R. China

Polyphenols, flavonoids, triterpenoids, and vitamin C from Clinacanthus nutans were extracted by an ultrasonic microwave-assisted method. The optimal extraction conditions were established in distilled water, solid-liquid ratio 1 : 55 g/ml, irradiation power 90 W, and extraction cycle with 75 seconds. Results revealed that the solid-liquid ratio exerted a significant effect on polyphenols, whereas the irradiation power played a key role in the extraction of flavonoids, triterpenoids, and vitamin C. Under these conditions the extraction yield of each component was: polyphenols 8.893, flavonoids 25 936, triterpenoids 16 789, and vitamin C 0.166 mg/g. These results showed that the ultrasonic microwave-assisted extraction was an efficient technology to extract bioactive substances from Clinacanthus nutans and Clinacanthus nutans could potentially be a source of natural antioxidants.

Keywords: herbaceous plant; bioactive substance; ultrasonic microwave-assisted extraction; yield

Published: February 28, 2017  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Yu Q, Li C, Duan Z, Liu B, Duan W, Shang F. Ultrasonic microwave-assisted extraction of polyphenols, flavonoids, triterpenoids, and vitamin C from Clinacanthus nutans. Czech J. Food Sci. 2017;35(1):89-94. doi: 10.17221/82/2016-CJFS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Chen Y., Gu X., Huang S.Q. (2010): Optimization of ultrasonic-microwave assisted extraction (UMAE) of polysaccharides from inonotus obliquus and evaluation of its anti-tumor activities. International Journal of Biological Macromolecules, 46: 429-435. Go to original source... Go to PubMed...
    2. Cristina L., Timothy J.M. (2010): Microwave and ultrasonic processing: now a realistic option for industry. Chemical Engineering and Processing, 49: 885-900. Go to original source...
    3. Liu H.W., Chen H.X., Gong X.Y. (2014): Study on microwave and ultrasonic-assisted extraction of flavonoids in the sweet potapo leaves. Science and Technology of Food Industry, 16: 298-301.
    4. Liu Q.D. (2010): Technological study on synergistic extraction of celery flavonoids by ultrasonic wave and microwave. Food and Machinery, 26: 134-137.
    5. Sakdaart S., ShuyProm A., Pientong C. (2009): Bioactive constituents from the leaves of Clinacanthus nutans Lindau. Bioorganic and Medicinal Chemistry letters, 17: 1857-1860. Go to original source... Go to PubMed...
    6. Shan Q.L., Gek C.N., Rozita Y., Wen H.T. (2016): Sequential ultrasound-microwave assisted acid extraction (UMAE) of pectin from pomelo peels. International Journal of Biological Macromolecules, 93: 426-435. Go to original source... Go to PubMed...
    7. Singleton V.L., Rossi J.A. (1965): Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16: 144-158. Go to original source...
    8. Stephane G., Pierre B., Pascaline A. (2005): Rapid determination of polyphenols and Vitamin C in plant-derived products. Journal of Agricultural and Food Chemistry, 53: 1370-1373. doi: 10.17221/82/2016-CJFS Go to original source...
    9. Tang X., Zhu D., Huai W.B., Zhang W. (2017): Simultaneous extraction and separation of flavonoids and alkaloids from Crotalaria sessiliflora L. by microwave-assisted cloud-point extraction. Seperation and Purification Technology, 175: 266-273. Go to original source...
    10. Teshima K.I., Kaneko T., Ohtani K., Kasai R., Lhieochaiphant S., Picheansoonthon C., Yamasaki K. (1997): C-Glycosyl flavones from Clinacanthus nutans. Natural Medicines, 51: 557.
    11. Wang F., Zhao M., Xue X.L. (2012): Study on extraction process of polysaccharide from Chrysanthemum morifolium Ramat. Northern Horticulture, 2: 28-31.
    12. Wang H., Zhang W.M. (1995): Study on quality control of flavonoids in Ginkgo biloba leaves. Chinese Wild Plant Resources, 1: 13-16.
    13. Wang Q.H., Liu C., Fang B. (2010): Study on extraction process of flavonoids from peanut shell. Food Science and Technology, 35: 207-210.
    14. Wang X.E., Zhong X.W., Zhang W.X. (2013): The study on the chemical ingredients from Clinacanthus nutans and analyse its antitumor effect. China Pharmacy, 24: 4104-4107.
    15. Yao S.J., Huang S.Q., Chen Z.Y., Zeng Q.X. (2009): Ultrasonic-assisted extraction of the triterpenoids from Ganoderma lucidum. Modern Food Science and Technology, 25: 1015-1019.
    16. Yi B., Xu W.T., Deng D. (2012): Analysis of amino acids, trace elements and chemical constituents from the leaves of Clinacanthus nutans. Pharmaceutical Journal of Chinese People's Liberation Army, 28: 396.
    17. Yin Y., Ye Q., Lu Z.C. (2015): Study on ultrasonic-assisted extraction process of polysaccharide from Morinda officinalis. Northern Horticulture, 12: 124-126.
    18. Zhang W.H., Ma Z., Sun Y. (2014): Optimization of ultrasonic-assisted extraction process for total flavonoids from Clinacanthus nutans and evaluation of its in vitro antioxidant activity. Chinese Journal of Experimental Traditional Medical Formulae, 20: 38-41.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content