Czech J. Food Sci., 2016, 34(4):300-305 | DOI: 10.17221/375/2015-CJFS

Effects of a bilberry preparation on selected cell lines of the digestive systemFood Analysis, Food Quality and Nutrition

Kamila Borowiec1, Dominik Szwajgier1, Anna Olejnik2, Katarzyna Kowalska2, Zdzisław Targoński1
1 Department of Biotechnology, Human Nutrition and Science of Food Commodities, Faculty of Food Science and Biotechnology, University of Life Sciences, Lublin, Poland
2 Department of Biotechnology and Food Microbiology, Faculty of Food Science and Nutrition Poznań University of Life Sciences, Poznań, Poland

Bilberry is a valuable wild forest fruit harvested in many countries in Europe. The biological activities of bilberry include antioxidant, anticancer, antiviral, antibacterial, and anticholinesterase activities. This study examines the protective effects of a bilberry (BB) preparation on IEC-6, Caco-2, and HepG2 cell lines. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to study the cytotoxicity of BB. The genotoxicity was determined using single-cell microgel electrophoresis. The Ames test was employed to assay bilberry mutagenicity. No significant effects of BB (12.5-100 µg dry mass/ml) were observed on the viability of IEC-6, Caco-2, and HepG2 cells. There were no differences in the percentage of DNA in the comet tail between the cells treated with BB (100 µg dry mass/ml) and the control cells. However, a significant reduction of oxidative DNA damage in the HepG2 cells was found. BB exhibited neither mutagenic nor promutagenic effects. Our results suggest that bilberry can be a potential tool in the prevention of chronic diseases, without any undesired effects on the cells of the gastrointestinal tract.

Keywords: Vaccinium myrtillus L.; cytotoxicity; functional food; genotoxicity; mutagenicity

Published: August 31, 2016  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Borowiec K, Szwajgier D, Olejnik A, Kowalska K, Targoński Z. Effects of a bilberry preparation on selected cell lines of the digestive system. Czech J. Food Sci. 2016;34(4):300-305. doi: 10.17221/375/2015-CJFS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Aaby K., Grimmer S., Holtung L. (2013): Extraction of phenolic compounds from bilberry (Vaccinium myrtillus L.) press residue: Effects on phenolic composition and cell proliferation. LWT-Food Science and Technology, 54: 257-264. Go to original source...
    2. Balimane P.V., Chong S. (2005): Cell culture-based models for intestinal permeability: a critique. Drug Discovery Today, 10: 335-343. Go to original source... Go to PubMed...
    3. Borowiec K., Szwajgier D., Targoński Z., Demchuk O.M., Cybulska J., Czernecki T., Malik A. (2014): Cholinesterase inhibitors isolated from bilberry fruit. Journal of Functional Foods, 11: 313-321. Go to original source...
    4. Cyplik P., Marecik R., Piotrowska-Cyplik A., Olejnik A., Drożdżyńska A., Chrzanowski Ł. (2012): Biological denitrification of high nitrate processing wastewaters from explosives production plant. Water, Air and Soil Pollution, 223: 1791-1800. Go to original source... Go to PubMed...
    5. Hara S., Morita R., Ogawa T., Segawa R., Takimoto N., Suzuki K., Hamadate N., Hayashi S.M., Odachi A., Ogiwara I., Shibusawa S., Yoshida T., Shibutani M. (2014): Tumor suppression effects of bilberry extracts and enzymatically modified isoquercitrin in early preneoplastic liver cell lesions induced by piperonyl butoxide promotion in a two-stage rat hepatocarcinogenesis model. Experimental and Toxicologic Pathology, 66: 225-234. Go to original source... Go to PubMed...
    6. Hartmann A., Agurell E., Beevers C., Brendler-Schwaab S., Burlinson B., Clay P., Collins A., Smith A., Speit G., Thybaud V., Tice R.R. (2003): Recommendations for conducting the in vivo alkaline Comet assay. 4th International Comet Assay Workshop. Mutagenesis, 18: 45-51. Go to original source... Go to PubMed...
    7. Juadjur A., Mohn C., Schantz M., Baum M., Winterhalter P., Richling E. (2015): Fractionation of an anthocyanin-rich bilberry extract and in vitro antioxidative activity testing. Food Chemistry, 167: 418-424. Go to original source... Go to PubMed...
    8. Kausar H., Jeyabalan J., Aqil F., Chabba D., Sidana J., Singh I.P., Gupta R.C. (2012): Berry anthocyanidins synergistically suppress growth and invasive potential of human nonsmall-cell lung cancer cells. Cancer Letters, 325: 54-62. Go to original source... Go to PubMed...
    9. Kolehmainen M., Mykkänen O., Kirjavainen P.V., Leppänen T., Moilanen E., Adriaens M., Laaksonen D.E., Hallikainen M., Puupponen-Pimiä R., Pulkkinen L., Mykkänen H., Gylling H., Poutanen K., Törrönen R. (2012): Bilberries reduce low-grade inflammation in individuals with features of metabolic syndrome. Molecular Nutrition & Food Research, 56: 1501-1510. Go to original source... Go to PubMed...
    10. Madihi Y., Merrikhi A., Baradaran A., Ghobadi S., Shahinfard N., Ansari R., Karimi A., Mesripour A., RafieianKopaei M. (2013): Bioactive components and the effect of hydroalcoholic extract of Vaccinium myrtillus on postprandial atherosclerosis risk factors in rabbits. Pakistan Journal of Medical Sciences, 29: 384-389. Go to original source...
    11. Maron D.M., Ames B.N. (1983): Revised methods for the Salmonella mutagenicity test. Mutation Research, 113: 173-215. Go to original source... Go to PubMed...
    12. Mosmann T. (1983): Rapid colorimetric assay for estimation for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65: 55-63. Go to original source... Go to PubMed...
    13. Olejnik A.M., Marecik R., Białas W., Cyplik P., Grajek W. (2010): In vitro studies on atrazine effects on human intestinal cells. Water, Air and Soil Pollution, 213: 401-411. Go to original source...
    14. Olejnik A., Rychlik J., Kidoń M., Czapski J., Kowalska K., Juzwa W., Olkowicz M., Dembczyński R., Moyer M.P. (2016): Antioxidant effects of gastrointestinal digested purple carrot extract on the human cells of colonic mucosa. Food Chemistry, 190: 1069-1077. Go to original source... Go to PubMed...
    15. Raudsepp P., Anton D., Roasto M., Meremäe K., Pedastsaar P., Mäesaar M., Raal A., Laikoja K., Püssa T. (2013): The antioxidative and antimicrobial properties of the blue honeysuckle (Lonicera caerulea L.), Siberian rhubarb (Rheum rhaponticum L.) and some other plants, compared to ascorbic acid and sodium nitrite. Food Control, 31: 129-135. Go to original source...
    16. Schantz M., Mohn C., Baum M., Richling E. (2010): Antioxidative efficiency of an anthocyanin rich bilberry extract in the human colon tumor cell lines Caco-2 and HT-29. Journal of Berry Research, 1: 25-33. Go to original source...
    17. Seifried H.E., Seifried R.M., Clarke J.J., Junghans T.B., San R.H.C. (2006): A compilation of two decades of mutagenicity test results with the Ames Salmonella typhimurium and L5178Y mouse lymphoma cell mutation assays. Chemical Research in Toxicology, 19: 627-644. Go to original source... Go to PubMed...
    18. Sekizawa H., Ikuta K., Mizuta K., Takechia S., Suzutani T. (2013): Relationship between polyphenol content and anti-influenza viral effects of berries. Journal of the Science of Food and Agriculture, 93: 2239-2241. Go to original source... Go to PubMed...
    19. Szwajgier D., Borowiec K. (2012): Screening for cholinesterase inhibitors in selected fruits and vegetables. Electronic Journal of Polish Agricultural Universities, 15, #06.
    20. Törrönen R., Kolehmainen M., Sarkkinen E., Poutanen K., Mykkänen H., Niskanen L. (2013): Berries reduce postprandial insulin responses to wheat and rye breads in healthy women. Journal of Nutrition, 143: 430-436. Go to original source... Go to PubMed...
    21. Valentová K., Ulrichová J., Cvak L., ©imánek V. (2007): Cytoprotective effect of a bilberry extract against oxidative damage of rat hepatocytes. Food Chemistry, 101: 912-917. Go to original source...
    22. Wilkening S., Bader A. (2003): Influence of culture time on the expression of drug-metabolising enzymes in primary human hepatocytes and hepatoma cell line HepG2. Journal of Biochemical and Molecular Toxicology, 17: 207-213. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content