Czech J. Food Sci., 2016, 34(2):180-188 | DOI: 10.17221/442/2015-CJFS

Effect of microwave modification on mechanical properties and structural characteristics of soy protein isolate and zein blended filmFood Technology and Economy, Engineering and Physical Properties

Na Wang1, Yu-Zhe Gao1, Peng Wang1, Shu Yang2, Tie-Min Xie1, Zhi-Gang Xiao1
1 College of Grain Science and Technology, Shenyang Normal University, Shenyang, Liaoning, P.R. China
2 College of Food, Shenyang Agricultural University, Shenyang, Liaoning, P.R. China

Soy protein isolate (SPI) and zein were blended at different ratios, and native and microwave-modified films were prepared at pH 12 in an ethanol/water (20 : 80) mixture. The effect of the microwave modification on the mechanical properties and structural characteristics of the SPI and zein blended films was investigated. The results show that the microwave-modified blended film of 3 : 1 SPI-zein demonstrated the best mechanical properties: the highest breaking strength (2900 g) and the highest fracturing distance (16.08 mm). The morphology of the microwave-modified film was more homogeneous and had fewer pinholes than the zein-only film. In addition, the glass transition temperature of the microwave-modified film rose by nearly half compared to the zein-only film, while the melting temperature increased by almost 2.5%. The secondary structure analysis indicates that a moderate amount of microwave treatment will promote a decrease in α-helix, β-turn, and random coil content and an increase in β-sheet content. This study provides an edible material with better flexibility for food packaging.

Keywords: protein film; protein modification; breaking strength; fracturing distance; structural characteristics

Published: April 30, 2016  Show citation

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Wang N, Gao Y, Wang P, Yang S, Xie T, Xiao Z. Effect of microwave modification on mechanical properties and structural characteristics of soy protein isolate and zein blended film. Czech J. Food Sci. 2016;34(2):180-188. doi: 10.17221/442/2015-CJFS.
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    References

    1. Biswas A., Selling G.W., Woods K.K., Evans K. (2009): Surface modification of zein films. Industrial Crops and Products, 30: 168-171. Go to original source...
    2. Chen L., Remondetto G.E., Subirade M. (2006): Food protein-based materials as nutraceutical delivery systems. Trends in Food Science & Technology, 17: 272-283. Go to original source...
    3. Chen Y.H., Yang J.T., Chau K.H. (1974): Determination of Helix and β form of proteins in aquous solution by circular dichroism. Biochemistry, 13: 3350-3359. Go to original source... Go to PubMed...
    4. Chen Y., Ye R., Li X., Wang J. (2013): Preparation and characterization of extruded thermoplastic zein-poly(propylene carbonate) film. Industrial Crops and Products, 49: 81-87. Go to original source...
    5. Chinnadayyala S.S.R., Santhosh M., Goswami P. (2012): Microwave based reversible unfolding and refolding of alcohol oxidase protein probed by fluorescence and circular dichroism spectroscopy. Journal of Biophysical Chemistry, 3: 317. Go to original source...
    6. Copty A.B., Neve-Oz Y., Barak I., Golosovsky M., Davidov D. (2006): Evidence for a specific microwave radiation effect on the green fluorescent protein. Biophysical Journal, 91: 1413-1423. Go to original source... Go to PubMed...
    7. Cunningham P., Ogale A., Dawson P., Acton J. (2000): Tensile properties of soy protein isolate films produced by a thermal compaction technique. Journal of Food Science, 65: 668-671. Go to original source...
    8. Gao Z., Ding P., Zhang L., Shi J., Yuan S., Wei J., Chen D. (2007): Study of a Pingyangmycin delivery system: Zein/ Zein-SAIB in situ gels. International Journal of Pharmaceutics, 328: 57-64. Go to original source... Go to PubMed...
    9. Ghanbarzadeh B., Oromiehie A., Musavi M. (2006): Effect of plasticizing sugars on rheological and thermal properties of zein resins and mechanical properties of zein films. Food Research International, 39: 882-890. Go to original source...
    10. Gu L., Wang M., Zhou J. (2013): Effects of protein interactions on properties and microstructure of zein-gliadin composite films. Journal of Food Engineering, 119: 288-298. Go to original source...
    11. Guo H., Heinämäki J., Yliruusi J. (2008): Stable aqueous film coating dispersion of zein. Journal of Colloid and Interface Science, 322: 478-484. Go to original source... Go to PubMed...
    12. Hua Y.F., De Ni P., Gu W.Y., Shen B.Y. (1996): Mechanism of physical modification of insoluble soy protein concentrate. Journal of the American Oil Chemists' Society, 73: 1067-1070. Go to original source...
    13. Johnson W.C. (1990): Protein secondary structure and circular dichroism: a practical guide. Proteins: Structure, Function, and Bioinformatics, 7: 205-214. Go to original source... Go to PubMed...
    14. Kara F., Chang C., Michael N. (2015): Incorporation of phenolic compounds, rutin and epicatechin, into soy protein isolate films: Mechanical, barrier and cross-linking properties. Food Chemistry, 172: 18-23. Go to original source... Go to PubMed...
    15. Kim S., Sessa D., Lawton J. (2004): Characterization of zein modified with a mild cross-linking agent. Industrial Crops and Products, 20: 291-300. Go to original source...
    16. Kokoszka S., Debeaufort F., Hambleton A., Lenart A., Voilley A. (2010): Protein and glycerol contents affect physico-chemical properties of soy protein isolate-based edible films. Innovative Food Science & Emerging Technologies, 11: 503-510. Go to original source...
    17. Kumar P., Sandeep K.P., Alavi S., Truong V.D., Gorga R.E. (2010): Preparation and characterization of bionanocomposite films based on soy protein isolate and montmorillonite using melt extrusion. Journal of Food Engineering, 100: 480-489. Go to original source...
    18. Kurouski D., Luo H., Sereda V., Robb F.T., Lednev I.K. (2012): Rapid degradation kinetics of amyloid fibrils under mild conditions by an archaeal chaperonin. Biochemical and Biophysical Research Communications, 422: 97-102. Go to original source... Go to PubMed...
    19. Li J., Zu Y.-G., Luo M., Gu C.-B., Zhao C.-J., Efferth T., Fu Y.-J. (2013): Aqueous enzymatic process assisted by microwave extraction of oil from yellow horn (Xanthoceras sorbifolia Bunge) seed kernels and its quality evaluation. Food Chemistry, 138: 2152-2158. Go to original source... Go to PubMed...
    20. Rhim J.-W., Mohanty K.A., Singh S.P., Ng P.K. (2006): Preparation and properties of biodegradable multilayer films based on soy protein isolate and poly (lactide). Industrial & Engineering Chemistry Research, 45: 3059-3066. Go to original source...
    21. Santosa F.B., Padua G.W. (2000): Thermal behavior of zein sheets plasticized with oleic acid. Cereal Chemistry, 77: 459-462. Go to original source...
    22. Schmidt V., Giacomelli C., Soldi V. (2005): Thermal stability of films formed by soy protein isolate-sodium dodecyl sulfate. Polymer Degradation and Stability, 87: 25-31. Go to original source...
    23. Shi J.-H., Wang J., Zhu Y.-Y., Chen J. (2014): Characterization of interaction between isoliquiritigenin and bovine serum albumin: Spectroscopic and molecular docking methods. Journal of Luminescence, 145: 643-650. Go to original source...
    24. Shi K., Huang Y., Yu H., Lee T.C., Huang Q. (2011): Reducing the brittleness of zein films through chemical modification. Journal of Agricultural and Food Chemistry, 59: 56-61. Go to original source... Go to PubMed...
    25. Shukla R., Cheryan M. (2001): Zein: the industrial protein from corn. Industrial Crops and Products, 13: 171-192. Go to original source...
    26. Sreerama N., Venyaminov S.Y., Woody R.W. (2000): Estimation of protein secondary structure from circular dichroism spectra: inclusion of denatured proteins with native proteins in the analysis. Analytical Biochemistry, 287: 243-251. Go to original source... Go to PubMed...
    27. Surewicz W.K., Mantsch H.H., Chapman D. (1993): Determination of protein secondary structure by Fourier transform infrared spectroscopy: a critical assessment. Biochemistry, 32: 389-394. Go to original source... Go to PubMed...
    28. Swain S., Biswal S., Nanda P., Nayak P.L. (2004): Biodegradable soy-based plastics: opportunities and challenges. Journal of Polymers and the Environment, 12: 35-42. Go to original source...
    29. Trinetta V., Cutter C., Floros J. (2011): Effects of ingredient composition on optical and mechanical properties of pullulan film for food-packaging applications. LWT-Food Science and Technology, 44: 2296-2301. Go to original source...
    30. Voutsinas L.P., Cheung E., Nakai S. (1983): Relationships of hydrophobicity to emulsifying properties of heat denatured proteins. Journal of Food Science, 48: 26-32. Go to original source...
    31. Wang Z., Sun X.-X., Lian Z.-X., Wang X.-X., Zhou J., Ma Z.-S. (2013): The effects of ultrasonic/microwave assisted treatment on the properties of soy protein isolate/microcrystalline wheat-bran cellulose film. Journal of Food Engineering, 114: 183-191. Go to original source...
    32. Whitmore L., Wallace B.A. (2008): Protein secondary structure analyses from circular dichroism spectroscopy: methods and reference databases. Biopolymers, 89: 392-400. Go to original source... Go to PubMed...
    33. Yang J.T., Wu C.-S., Martinez H.M. (1986): Calculation of protein conformation from circular dichroism. Methods in Enzymology, 130: 208-269. Go to original source... Go to PubMed...
    34. Zhong Q., Jin M., Xiao D., Tian H., Zhang W. (2008): Application of supercritical anti-solvent technologies for the synthesis of delivery systems of bioactive food components. Food Biophysics, 3: 186-190. Go to original source...
    35. Zubov V.P., Chikhacheva I.P., Nikolaeva E.I., Kapustin D.V., Yagudaeva E.Y., Kubrakova I.V. (2009): Microwave-assisted synthesis of composite sorbents on the basis of silica modified by polyvinyl alcohol. Russian Journal of General Chemistry, 79: 191-194. Go to original source...

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