Czech J. Food Sci., 2016, 34(1):47-51 | DOI: 10.17221/254/2015-CJFS
Relationship between somatic cell count and milk casein level obtained by two different methodsFood Technology and Economy, Engineering and Physical Properties
- 1 Department of Anatomy and Physiology, Faculty of Veterinary Medicine
- 2 and Department of Animal Breeding and Nutrition, Faculty of Animal Husbandry Technology, Lithuanian University of Health Sciences, Kaunas, Lithuania
The purpose of the present work was to investigate the impact of somatic cell count (SCC) on the content of milk casein and to compare the sensitivity of different methods for the determination of the casein level in milk. Two different methods were employed in order to determine the casein level: routine method - infrared spectrophotometry and reference method - Kjeldahl. Data array of 26, 169 milk samples of the livestock control was used to analyse milk casein variation by SCC limits. Mean casein values (investigated by the routine method) varied from 2.47% to 2.8% (2.65 ± 0.09) and casein number (in % on crude protein basis) - CN% varied from 79.5% to 81.1%. The present analysis showed that the relation between SCC and casein content obtained by the routine method correlated significantly (Pearson correlation rp = 0.644, P < 0.05, R2 = 0.414, Spearman correlation rs = 0.786, P < 0.05). Evaluated results obtained by the least squares method, and linear approximation of variation indicators presented a noticeable decline in casein percentage - 0.27%, when SCC increased by 80 × 103/ml, but only when the studies were done by the reference method.
Keywords: raw milk; proteins; cow
Published: February 29, 2016 Show citation
References
- Akers R.M. (2002): Lactation and the Mammary Gland. Iowa, Iowa State Press: 100-200.
- Auldist M.J., Hubble I.B. (1998): Effects of mastitis on raw milk and dairy products. Australian Journal of Dairy Technology, 53: 28-36.
- Ballou L.U., Pasquini M., Bremel R.D., Everson T., Dean Somme R.D. (1995): Factors affecting herd milk composition and milk plasmin at four levels of somatic cell counts. Journal of Dairy Science, 78: 2186-2195.
Go to original source...
Go to PubMed...
- Barbano D.M. (2000): Practical Guide for Control of Cheese Yield. International Dairy Federation, Brussels: 19-27.
- Barbano D.M., Dellavalle M.E. (1987): Rapid method for determination of milk casein content by infrared analysis. Journal of Dairy Science, 70: 1524-1528.
Go to original source...
Go to PubMed...
- Barbano D.M., Rasmussen R.R., Lynch J.M. (1991): Influence of milk somatic cell count and milk age on cheese yield. Journal of Dairy Science, 74: 369-388.
Go to original source...
- Barlowska J., Litwinczuk Z., Wolanciuk A., Brodziak A. (2009): Relationship of somatic cell count to daily yield and technological usefulness of milk from different breeds of cows. Polish Journal of Veterinary Science, 12: 75-79.
- Bastian E.D., Brown R.J. (1996): Plasmin in milk and dairy products: an update. International Dairy Journal, 6: 435-457.
Go to original source...
- Button P.D., Roginski H., Deeth H.C., Craven H.M. (2011): Improved shelf life estimation of UHT milk by prediction of proteolysis. Journal of Food Quality, 34: 229-235.
Go to original source...
- Coulon J.B., Hurtaud C., Remond B., Verite R. (1998): Factors contributing to variation in the proportion of casein to cows' milk true protein: a review of recent INRA experiments. Journal of Dairy Research, 65: 375-387.
Go to original source...
Go to PubMed...
- Coulon J.B., Gasqui P., Barnouin J., Ollier A., Pradel P., Pomiès D. (2002): Effect of mastitis and related-germ on milk yield and composition during naturally-occurring udder infections in dairy cows. Animal Research, 51: 383-393.
Go to original source...
- Cunha R.P.L., Molina L.R., Carvalho A.U., Facury Filho E.J., Ferreira P.M., Gentilini M.B. (2008): Subclinical mastitis and relationship between somatic cell count with number of lactations, production and chemical composition of milk. Brazilian Journal of Veterinary and Animal Science, 60: 19-24.
Go to original source...
- Hallén E. (2008): Coagulation Properties of Milk Association with Milk Protein Composition and Genetic Polymorphism. [PhD Thesis.]. Uppsala, Swedish University of Agricultural Sciences.
- Hanuš O., Yong T., Kučera J., Genčurová V., Hanušová K., Kopec T., Kopecký J., Jedelská R. (2010): Analýza výsledků kalibrací pro stanovení kaseinu nepřímou metodou infračevené spektroskopie. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 58: 123-136.
Go to original source...
- Le Bars D., Gripon J.C. (1993): Hydrolysis of α-s1-casein by bovine plasmin. Lait, 73: 337-344.
Go to original source...
- Le Roux Y., Colin O., Laurent F. (1995): Proteolysis in samples of quarter milk with varying somatic cell counts. 1. Comparison of some indicators of endogenous proteolysis in milk. Journal of Dairy Science, 78: 1289-1297.
Go to original source...
Go to PubMed...
- Le Roux Y., Laurent F., Moussaou F. (2003): Polymorphonuclear proteolytic activity and milk composition change. Veterinary Research, 34: 629-645.
Go to original source...
Go to PubMed...
- Litwińczuk Z., Król J., Brodziak A., Barłowska J. (2011): Changes of protein content and its fractions in bovine milk from different breeds subject to somatic cell count. Journal of Dairy Science, 94: 684-691.
Go to original source...
Go to PubMed...
- McSweeney P.L.H., Fox P.F. (1995): Proteolysis of bovine caseins by cathepsin D: preliminary observations and comparison with chymosin. International Dairy Journal, 5: 321-336.
Go to original source...
- Tripaldi C., Terramoccia S., Bartocci S., Angelucci M., Danese V. (2003): The effects of the somatic cell count on yield, composition and coagulating properties of mediterranean buffalo milk. Asian-Australasian Journal of Animal Sciences, 16: 738-742.
Go to original source...
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