Czech J. Food Sci., 2016, 34(1):32-38 | DOI: 10.17221/316/2015-CJFS

The occurrence of ochratoxin A in white and parboiled riceFood Chemistry and Safety

Jakub Toman1, František Malíř1, Vladimír Ostrý2, Yann Grosse3, Vladimír Dvořák1, Tomáš Roubal4, Lucie Neuchlová1
1 Department of Biology, Faculty of Science, University of Hradec Králové, Hradec Králové, Czech Republic
2 Center of Health, Nutrition and Food, National Institute of Public Health, Brno, Czech Republic
3 International Agency for Research on Cancer, Lyon, France
4 Regional Branch Hradec Králové, Institute of Public Health in Ústí nad Labem, Hradec Králové, Czech Republic

The levels of ochratoxin A (OTA), one of the five agricultural and toxicological most important mycotoxins, have been determined by HPLC with fluorescence detection (HPLC-FLD) or enzyme-linked immunosorbent assay (ELISA) in 60 samples of white and parboiled rice, purchased on the Czech food market. In all 60 samples of rice tested by HPLC-FLD. OTA levels were below the limit of quantification (LOQ), i.e. < 0.2 ng/g. The same samples were also tested by ELISA (LOQ = 0.05 ng/g), and 58 samples (96.7%) were found to be positive. OTA levels in white and parboiled rice ranged from 0.05 to 0.17 ng/g; the arithmetic mean for all samples was 0.15 ng/g, the median 0.15 ng/g, and the 90% percentile 0.17 ng/g. These findings confirm the necessity of monitoring OTA in different foodstuffs and food, due to the precautionary principle and the uncertainty in the genotoxicity status of OTA.

Keywords: mycotoxins; white rice; foodstuffs; determination; monitoring; HPLC; ELISA

Published: February 29, 2016  Show citation

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Toman J, Malíř F, Ostrý V, Grosse Y, Dvořák V, Roubal T, Neuchlová L. The occurrence of ochratoxin A in white and parboiled rice. Czech J. Food Sci. 2016;34(1):32-38. doi: 10.17221/316/2015-CJFS.
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References

  1. Aydin A., Aksu H., Gunsen U. (2011): Mycotoxin levels and incidence of mould in Turkish rice. Environmental Monitoring and Assessment, 178: 271-280. Go to original source... Go to PubMed...
  2. Almeidaa M.I., Almeidaa N.G., Carvalhoa K.L., Gongalvesa G.A.A., Silvaa C.N., Santosa E.A., Garciaa J.C., Vargas E.A. (2012): Co-occurrence of aflatoxins B1, B2, G1 and G2, ochratoxin A, zearalenone, deoxynivalenol, and citreoviridin in rice in Brazil. Food Additives and Contaminants: Part A, 4: 694-703. Go to original source... Go to PubMed...
  3. Bansal J., Pantazopoulos P., Tam J., Cavlovi P., Kwongk Turcotte A.-M., Lau B.P.-Y., Scott P.M. (2011): Surveys of rice sold in Canada for aflatoxins, ochratoxin A and fumonisins. Food Additives and Contaminants: Part A, 28: 767-774. Go to original source... Go to PubMed...
  4. Buyukunal S. K., Kahraman T., Ciftcioglu G.R. (2010): Occurrence of AF, AFB 1, OTA in rice commercialized in eastern Turkey. Polish Journal of Environmental Studies, 19: 907-912.
  5. Coelho C.S., Badiale-Furlong E., Almeida T.L. (1999): Migraçăo de micotoxinas durante o processo de parboilizaçăo do arroz. Brazilian Journal Food Technology, 2: 43-50.
  6. ČSÚ (2012): Analýza spotřeby potravin v roce 2010. Prague.
  7. Dohnal V., Dvorak V., Malir F., Ostry V., Roubal T. (2013): A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic. Food and Chemical Toxicology, 62: 427-431. Go to original source... Go to PubMed...
  8. Dors G.C., Pinto L.A.A., Badiale-Furlong E. (2009): Migration of mycotoxins into rice starchy endosperm during the parboiling process. Food Science and Technology, 42: 433-437. Go to original source...
  9. Dors G.C., Bierhals V.S., Badiale-Furlong E. (2011): Parboiled rice: chemical composition and the occurrence of mycotoxins. Food Science and Technology, 31: 172-177. Go to original source...
  10. EN ISO/IEC 17025 (2005): Conformity assessment - General reguirements for the competence of testing and calibration laboratories. Geneva, ISO Copyright Office.
  11. European Union (2006): Commission Regulation (EC) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs (Text with EEA relevance) (2006): Official Journal of European Union, L 364: 5-24.
  12. FAOSTAT (Statistics at Food and Agriculture Organization of the United Nations) (2015): World maize, wheat, rice, soybeans, potatoes and cotton production. Available at http://faostat3.fao.org/browse/Q/QC/E (accessed June 22, 2015).
  13. Feizy J., Beheshti H.R., Fakoor Janati S.S., Fahim N.K. (2011): Survey of ochratoxin A in rice from Iran using affinity column cleanup and HPLC with fluorescence detection. Food Additives and Contaminants: Part B, 4: 67-70. Go to original source... Go to PubMed...
  14. Frelund E., Thimb A.M., Gilund A., Brostedt S., Nyberg M., Olsen M. (2009): Moulds and mycotoxins in rice from the Swedish retail market. Food Additives and Contaminants: Part A, 26: 527-533. Go to original source... Go to PubMed...
  15. Gekle M., Sauvant C., Schwerdt G. (2005): Ochratoxin A at nanomolar concentrations: A signal modulator in renal cells. Molecular Nutrition Food Research, 49: 118-130. Go to original source... Go to PubMed...
  16. Ghalia R., Hmaissia-Khlifaa K., Ghorbel H., Maaroufi K., Hedilia A. (2008): Incidence of aflatoxins, ochratoxin A and zearalenone in tunisian foods. Food Control, 9, 921-924. Go to original source...
  17. Gomes L.B., Ward T.J., Badiale-Furlong E., Del Ponte E.M. (2015): Species composition, toxigenic potential and pathogenicity of Fusarium graminearum species complex isolated from southern Brasilian rice. Plant Pathology, 64: 980-987. Go to original source...
  18. IARC (International Agency for Research on Cancer) (1993): IARC Monographs on the Evaluation of Carcinogenic Risks to Humans: Some Naturally Occurring Substances: Food Items and Constituents, Heterocyclic Aromatic Amines and Mycotoxins. Vol. 56. Lyon, IARC.
  19. Igarashi N., Nakamura M., Watai M. (2008): Application of the AOAC official method of analysis for determination of ochratoxin A in brown rice-interlaboratory study. Mycotoxins, 58: 97-105. Go to original source...
  20. ISO 13528 (2005): Statistical methods for use in proficiency testing by interlaboratory comparisons. Geneva, International Organisation for Standardization: 1-66.
  21. Iqbal S.Z., Asi M.R., Zuber M., Akhtar J., Saif M.J. (2013): Natural occurrence of aflatoxins and ochratoxin A in commercial chilli and chilli sauce samples. Food Control, 30: 621-625. Go to original source...
  22. JECFA (2008): Ochratoxin A. In: Safety evaluation of certain food additives and contaminants. Sixty-eighth meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), Geneva, Switzerland. WHO Food Additives Series, 59: 169-180.
  23. Juan C., Zinedine A., Idrissi L., Manes J. (2008): Ochratoxin A in rice on the Moroccan retail market. International Journal of Food Microbiology, 1-2: 83-85. Go to original source... Go to PubMed...
  24. Lai X.-W., Liu R., Ruan C.-Q., Zhang H., Liu C.-L. (2014a): Occurrence of aflatoxins and ochratoxin A in rice samples from six provinces in China. Food Control, 50: 401-404. Go to original source...
  25. Lai X.-W., Liu R., Sun D.-L., Ruan C.-Q., Zhang H., Liu C.-L. (2014b): Rapid analysis of aflatoxins B1, B2, and ochratoxin A in rice samples using dispersive liquid- liquid microextraction combined with HPLC. Journal of Separation Science, 37: 92-98. Go to original source... Go to PubMed...
  26. Lee H.J., Ok H.E., Sim J.H., Min S.G., Chun H.S. (2015): Estimate of zearalenone exposure through the intake of white and brown rice in the Korean population. Toxicological and Environmental Chemistry, 97: 1071-1085. Go to original source...
  27. Makun H.A., Dutton M.F., Njobeh P.B., Mwanza M., Kabiru A.Y. (2011): Natural multi-occurrence of mycotoxins in rice from Niger State, Nigeria. Mycotoxin Research, 27: 97-104. Go to original source... Go to PubMed...
  28. Malir F., Ostry V., Grosse Y., Roubal T., Skarkova J., Ruprich J. (2006): Monitoring the mycotoxins in food and their biomarkers in the Czech Republic. Molecular Nutrition Food Research, 50: 513-518. Go to original source... Go to PubMed...
  29. Malir F., Ostry V., Pfohl-Leszkowicz A., Roubal T. (2012): Ochratoxin A exposure biomarkers in the Czech Republic and comparison with foreign countries. Biomarkers, 17: 577-589. Go to original source... Go to PubMed...
  30. Malir F., Ostry V., Novotna E. (2013a): Toxicity of the mycotoxin ochratoxin A in the light of recent data. Toxin Review, 32: 19-33. Go to original source...
  31. Malir F., Ostry V., Pfohl-Leszkowicz A., Novotna E. (2013b): Ochratoxin A: developmental and reproductive toxicity - an overview. Birth Defects Research Part B: Developmental and Reproductive Toxicology, 98: 493-502. Go to original source... Go to PubMed...
  32. Mally A. (2012): Ochratoxin A and mitotic disruption: mode of action analysis of renal tumor formation by ochratoxin A. Toxicological Sciences, 127: 315-330. Go to original source... Go to PubMed...
  33. Margo A., Carvalho M.O., Bastos M.S.M., Carolino M., Adler C.S., Timlick B., Mexia A. (2006): Mycoflora of stored rice in Portugal. In: 9 th International Working Conference on Stored Product Protection, Oct 15-18, 2006, Campinas, Săo Paulo, Brazil: 128-134.
  34. Meija D. (2003): Post-harvest Operations Compendium. Rome, AGSI/FAO.
  35. Mosayebi M., Mirzaee H. (2014): Determination of mycotoxin contamination and heavy metals in edible rice imported to Golestan Province. Iranian Journal of Health and Enviroment, 4: 503-514.
  36. Nazari F., Sulyok M., Kobarfard F., Yazdanpanah H., Krska R. (2015): Evaluation of emerging Fusarium mycotoxins beauvericin, enniatins, Fusaproliferin and moniliformin in domestic rice in Iran. Iranian Journal of Pharmaceutical Research, 14: 505-512.
  37. Nguyen M.T., Tozlovanu M., Tran T.L., Pfohl-Leszkowicz A. (2007): Occurrence of aflatoxin B1, citrinin and ochratoxin A in rice in five provinces of the central region of Vietnam. Food Chemistry, 105: 42-47. Go to original source...
  38. Official method 2000.03. (2002): Ochratoxin A in barley. AOAC International.
  39. Ortiz J., Van Camp J., Mestdagh F., Donoso S., De Meulenaer B. (2013): Mycotoxin co-occurrence in rice, oat flakes and wheat noodles used as staple foods in Ecuador. Food Additives and Contaminants: Part A, 30: 2165-2176. Go to original source... Go to PubMed...
  40. Ostry V., Malir F., Ruprich J. (2013): Producers and important dietary sources of ochratoxin A and citrinin. Toxins, 5: 1574-1586. Go to original source... Go to PubMed...
  41. Owens G. (2001): Cereals Processing Technology. Cambrigde, Woodhead Publishing Ltd. and CRC Press LLC. Go to original source...
  42. Ozden S., Akdeniz A.S., Alpertunga B. (2012): Occurrence of ochratoxin A in cereal-derived food products commonly consumed in Turkey. Food Control, 25: 69-74. Go to original source...
  43. Pena A., Cerejo F., Lino C., Silveria I. (2005): Determination of ochratoxin A in Portuguese rice samples by high performance liquid chromatography with fluorescence detection. Analytical and Bioanalytical Chemistry, 382: 1288-1293. Go to original source... Go to PubMed...
  44. Pfohl-Leszkowicz A., Tozlovanu M., Manderville R., Peraica M., Castegnaro M., Stefanovic V. (2007): New molecular and field evidences for the implication of mycotoxins but not aristolochic acid in human nephropathy and urinary tract tumor. Molecular Nutrition Food Research, 51: 131-146. Go to original source... Go to PubMed...
  45. Pfohl-Leszkowicz A., Manderville RA. (2012): An update on direct genotoxicity as a molecular mechanism of ochratoxin A carcinogenicity. Chemical Research in Toxicology, 25: 252-262. Go to original source... Go to PubMed...
  46. Rahimi E. (2014): The occurrence of ochratoxin A in rice in six provinces of Iran. Toxicology and Industrial Health, 30: 1-4.
  47. Rahmani A., Jinap S., Soleimany F. (2010): Validation of the procedure for the simultaneous determination of aflatoxins ochratoxin A and zearalenone in cereals using HPLC-FLD. Food Additives and Contaminants: Part A, 27: 1683-1693. Go to original source... Go to PubMed...
  48. R-Biopharm (2014): RIDASCREEN® Ochratoxin A 30/15: Enzyme Immunoassay for the Quantitative Analysis of Ochratoxin A. Darmstadt, R-Biopharm AG: 1-18.
  49. Rofiat A.-S., Fanelli F., Atanda O., Sulyok M., Cozzi G., Bavaro S., Krska R., Logrieco A.F., Ezekiel Ch.N. (2015): Fungal and bacterial metabolites associated with natural contamination of locally processed rice (Oryza sativa L.) in Nigeria. Food Additives and Contaminants: Part A, 32: 950-959. Go to original source... Go to PubMed...
  50. Sales A.C., Yoshizawa T. (2005): Updated profile of aflatoxin and Aspergillus section Flavi contamination in rice and its byproducts from the Philippines. Food Additives and Contaminants, 22: 429-436. Go to original source... Go to PubMed...
  51. Skarkova J., Ostry V., Malir F., Roubal T. (2013): Determination of ochratoxin A in food by high-performance of chromatography. Analytical Letters, 46: 1495-1504. Go to original source...
  52. Suarez-Bonnet E., Carvajal M., Mendez-Ramirez I., CastilloUrueta P., Cortes-Eslava J., Gomez-Arroyo S., Melero-Vara J.M. (2013): Aflatoxin (B1, B2, G1, and G2) contamination in rice of Mexico and Spain from local sources or imported. Journal of Food Science, 78: 1822-1829. Go to original source... Go to PubMed...
  53. Tanaka K., Sag, Y., Zheng Y. Z., Nakagawa H., Kushiro M. (2007): Mycotoxins in rice. International Journal of Food Microbiology, 119: 59-66. Go to original source... Go to PubMed...
  54. Venkataramana M., Rashmi R., Uppalati S.R., Chandranayaka S., Balakrishna K., Radhika M., Gupta V.K., Batra H.V. (2015): Development of sandwich dot-ELISA for specific detection of ochratoxin A and its application on to contaminated cereal grains originating from India. Frontiers in Microbiology, 6: 1-11. Go to original source... Go to PubMed...
  55. Xianwen L., Ruicen L., Chunqiang R., He Z., Chenglan L. (2015): Occurrence of aflatoxins and ochratoxin A in rice samples from six provinces in China. Food Control, 50: 401-404. Go to original source...
  56. Zhihong L., Kunlun H., Yunbo L. (2015): Ochratoxin A and ochratoxin-producing fungi on cereal grain in China: a review. Food Additives and Contaminants: Part A, 32: 461-470. Go to original source... Go to PubMed...

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