Czech J. Food Sci., 2016, 34(1):9-15 | DOI: 10.17221/307/2015-CJFS

Microbiological method using Bacillus megaterium with fusidic acid for detection of macrolides in milkFood Microbiology and Safety

Melisa Tumini1, Orlando G. Nagel1, Maria Pilar Molina2, Rafael L. Althaus1
1 Faculty of Veterinary Science, National University of the Littoral, Esperanza, Argentina
2 Institute for Animal Science and Technology, Universitat Politècnica de València, Valencia, Spain

The microbiological method to attain a sensitive detection of macrolides using Bacillus megaterium in agar medium with fusidic acid was designed. To this aim, Mueller-Hinton medium fortified with glucose at pH 8.0, a combination of redox indicators (brilliant black and toluidine blue) and different concentrations of fusidic acid were tested. The addition of fusidic acid in the culture medium improves the sensitivity of this bacteria test and decreases the detection limits of bioassay. The addition of 200 µg/lof fusidic acid detects 35 µg/lof erythromycin, 58 µg/lof tylosin, and 57 µg/lof tilmicosin in milk. This microbiological bioassay could be used as an alternative method of commercial screening test for detecting macrolides in milk, in order to maintain food safety.

Keywords: microbiological test; antibiotic; erytromycin; tylosin; tilmicosin

Published: February 29, 2016  Show citation

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Tumini M, Nagel OG, Molina MP, Althaus RL. Microbiological method using Bacillus megaterium with fusidic acid for detection of macrolides in milk. Czech J. Food Sci. 2016;34(1):9-15. doi: 10.17221/307/2015-CJFS.
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