Czech J. Food Sci., 2015, 33(4):313-319 | DOI: 10.17221/115/2015-CJFS

Fermentation of soymilk by yoghurt and bifidobacteria strainsFood Microbiology and Safety

©árka HORÁČKOVÁ1, Andrea MÜHLHANSOVÁ1, Marcela SLUKOVÁ2, Věra SCHULZOVÁ3, Milada PLOCKOVÁ1
1 Department of Dairy, Fat and Cosmetic Science,
2 Department of Carbohydrates and Cereals and

Soy and milk based products fermented by yoghurt culture YC-381 alone or in combination with two probiotic cultures (Bifidobacterium animalis subsp. lactis BB 12 and Bifidobacterium bifidum CCDM 94) were prepared. Bacteria growth, the amounts of lactic acid, acetic acid, and acetaldehyde were compared after 16 h of fermentation at 37°C. The changes of isoflavones concentrations were also monitored in soy products. The growth of Streptococcus thermophilus and bifidobacteria was similar in both media, but Lactobacillus delbrueckii subsp. bulgaricus showed a better growth in milk. Titratable acidity and the concentrations of acids were consistently higher in cow milk than in soymilk at the end of fermentation. Bifidobacteria, compared to the yoghurt culture, were only able to acidify the media to the half values. Comparing the bifidobacteria strains, Bifidobacterium animalis subsp. lactis BB 12 exhibited a better ability to acidify milk. The strain Bifidobacterium bifidum CCDM 94 was able to release 6.90 mg/100 ml of isoflavone aglycones in soymilk.

Keywords: acetaldehyde; acetic acid; isoflavones; lactic acid

Published: August 31, 2015  Show citation

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HORÁČKOVÁ ©, MÜHLHANSOVÁ A, SLUKOVÁ M, SCHULZOVÁ V, PLOCKOVÁ M. Fermentation of soymilk by yoghurt and bifidobacteria strains. Czech J. Food Sci. 2015;33(4):313-319. doi: 10.17221/115/2015-CJFS.
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    References

    1. Ang E.C., Boatright W.L. (2003): Olfactory perception of major odorants found in the headspace of aqueous soy protein isolate slurries. Journal of Food Science, 68: 388-393. Go to original source...
    2. Blagden T.D., Gilliland S.E. (2005): Reduction of levels of volatile components associated with the beany flavor in soymilk by Lactobacilli and Streptococci. Food Microbiology and Safety, 70: 186-189. Go to original source...
    3. Champagne C.P., Tompkins T.A., Buckley N.D., GreenJohnson J.N. (2010): Effect of fermentation by pure and mixed cultures of Streptococcus thermophiles and Lactobacillus helveticus on isoflavone and B-vitamin content of a fermented soy beverage. Food Microbiology, 27: 968-972. Go to original source... Go to PubMed...
    4. Chen T.R., Su R.Q., Wei Q.K. (2010): Hydrolysis of isoflavone phytoestrogens in soymilk fermented by Lactobacillus and Bifidobacterium cocultures. Journal of Food Biochemistry, 34: 1-12. Go to original source...
    5. Chumchuere S., Robinson R.K. (1999): Selection of starter cultures for the fermentation of soya milk. Food Microbiology, 16: 129-137. Go to original source...
    6. Desai A., Small D., McGill A.E.J., Shah N.P. (2002): Metabolism of raffinose and stachyose in reconstituted skim milk and of n-hexanal and pentanal in soymilk by bifidobacteria. Bioscience and Microflora, 21: 245-250. Go to original source...
    7. Ding W.K., Shah N.P. (2010): Enhancing the biotransformation of isoflavones in soymilk supplemented with lactose using probiotic bacteria during extended fermentation. Journal of Food Science, 75: 140-149. Go to original source... Go to PubMed...
    8. Donkor O.N., Henriksson A., Vasiljevic T., Shah N.P. (2007): α-Galactosidase and proteolytic activities of selected probiotic and dairy cultures in fermented soymilk. Food Chemistry, 104: 10-20. Go to original source...
    9. Farnworth E.R., Mainville I., Desjardins M.P., Gardner N., Fliss I., Champagne C. (2007): Growth of probiotic bacteria and bifidobacteria in soy yoghurt formulation. International Journal of Food Microbiology, 116: 174-181. Go to original source... Go to PubMed...
    10. Ishibashi N., Shimamura S. (1993): Bifidobacteria: research and development in Japan. Food Technology, 6: 126-136.
    11. Izumi T., Piskula M.K., Osawa S., Obata A., Tobe K., Saito M., Kataoka S., Kubota Y., Kikuchi M. (2000): Soy isoflavone aglycones are absorbed faster and in higher amounts than their glucosides in humans. Journal of Nutrition, 130: 1695-1699. Go to original source... Go to PubMed...
    12. Lankaputhra W.E.V., Shah N.P., Britz M.L. (1996): Survival of bifidobacteria during refrigerated storage in the presence of acid and hydrogen peroxide. Milchwissenschaft, 51: 65-70.
    13. Margolles A., Sánchez B. (2012): Selection of a Bifidobacterium animalis subsp. lactis strain with a decreased ability to produce acetic acid. Applied and Environmental Microbiology, 78: 3338-3342. Go to original source... Go to PubMed...
    14. Murti T.W., Roger S., Bouillianne C., Landon M., Desmazeaud M. (1992): Growth of Bifidobacterium sp. CNRZ 1494 in soybean milk and cow milk: effects on aroma compounds. Sciences des Aliments, 12: 429-439.
    15. Nosova T., Jousimies-Somer H., Jokelainen K., Heine R., Salaspuro M. (2000): Acetaldehyde production and metabolism by human indigenous and probiotic Lactobacillus and Bifidobacterium strains. Alcohol & Alcoholism, 35: 561-568. Go to original source... Go to PubMed...
    16. Ozer B., Atasoy F. (2002): Effect of addition of aminoacids, treatment with β-galactosidase and use of heat-shocked cultures on the acetaldehyde level in yoghurt. International Journal of Dairy Technology, 55: 166-170. Go to original source...
    17. Pinthong R., Macrae R., Rothwell J. (1980): The development of a soya-based yoghurt. I. Acid production by lactic acid bacteria. Journal of Food Technology 15: 647-652. Go to original source...
    18. Pyo Y.H., Lee T.C., Lee Y.C. (2005): Enrichment of bioactive isoflavones in soymilk fermented with β-glucosidaseproducing lactic acid bacteria. Food Research International, 38: 551-559. Go to original source...
    19. Rekha C.R., Vijayalakshmi G. (2010): Bioconversion of isoflavone glycosides to aglycones, mineral bioavailability and vitamin B complex in fermented soymilk by probiotic bacteria and yeast. Journal of Applied Microbiology, 109: 1198-1208. Go to original source... Go to PubMed...
    20. Scalabrini P., Rossi M., Spettoli P., Matteuzzi D. (1998): Characterization of Bifidobacterium strains for use in soymilk fermentation. International Journal of Food Microbiology, 39: 213-219. Go to original source... Go to PubMed...
    21. Setchell K.D.R., Brown N.M., Desai P., Zimmer-Nechemias L., Wolfe B.E., Brashear W.T., Kirschner A.S., Cassidy A., Heubi J.E. (2001): Bioavailability of pure isoflavones in healthy humans and analysis of commercial soy isoflavone supplements. Journal of Nutrition, 131: 1362-1375. Go to original source... Go to PubMed...
    22. Shah P. (2007): Functional cultures and health benefits. International Dairy Journal, 17: 1262-1277. Go to original source...
    23. Tsangalis D., Ashton J.F., McGill A.E.J., Shah N.P. (2003): Biotransformation of isoflavones by bifidobacteria in fermented soymilk supplemented with d-glucose and l-cysteine. Journal of Food Science, 68: 623-631. Go to original source...
    24. Tsangalis D., Shah N.P. (2004): Metabolism of oligosaccharides and aldehydes and production of organic acids in soymilk by probiotic bifidobacteria. International Journal of Food Science and Technology, 39: 541-554. Go to original source...
    25. Xanthopoulos V., Petridis D., Tyanetakis N. (2001): Characterization and classification of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus strains isolated from traditional Greek yoghurt. Food Microbiology and Safety, 66: 747-752. Go to original source...

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