Czech J. Food Sci., 2015, 33(3):283-291 | DOI: 10.17221/462/2014-CJFS

Physico-chemical and structural properties of four rice bran protein fractions based on the multiple solvent extraction methodFood Technology and Economy, Engineering and Physical Properties

Changyuan WANG1,2, Feng XU2, Dan LI2, Min ZHANG1,3
1 College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, P.R. China
2 College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, P.R. China
3 College of Food Science, Beijing Technology and Business University, Beijing, P.R. China

The physicochemical and structural properties of the concentrated rice bran protein (CRBP) and rice bran protein fractions - RBPF (albumin, globulin, prolamin, and glutelin) were investigated on the basis of multiple solvent extraction method. The protein fractions mainly consisted of essential amino acids except for prolamin. The amino acid composition of concentrated protein was superior to soybean protein isolation, such as valine, methionine, leucine, phenylalanine, and histidine, with similar characteristics of solubility, emulsification, and foaming. Based on the difference in amino acid composition, all these five proteins showed relatively high surface hydrophobicity more than 369.3 of prolamin. CRBP and RBPF were composed of different molecular subunits in SDS-PAGE profile. The circular dichroism spectra (CDS) in synergy showed that the primary structures of RBPF were β-protein folding and random coils with various denaturation temperatures in the range of 78.69°C for glutelin and 92.88°C for globulin. The fluorescence spectrometry assays showed that the blue shift occurred at 348 nm for globulin, while the red shift occurred for the concentrated protein, albumin, and globulin at 356.2, 348.6, and 347.0 nm, respectively. Therefore, the research presents the basic characterisation for further application of natural rice bran protein in the food industry.

Keywords: rice bran protein fractions; functional properties; multiple solvent extract

Published: June 30, 2015  Show citation

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WANG C, Feng X, Dan L, ZHANG M. Physico-chemical and structural properties of four rice bran protein fractions based on the multiple solvent extraction method. Czech J. Food Sci. 2015;33(3):283-291. doi: 10.17221/462/2014-CJFS.
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    References

    1. Adebiyi A.O., Hasegawa Y., Ogawa T., Muramoto K. (2009): Isolation and characterization of protein fractions from deoiled rice bran. European Food Research and Technology, 288: 391-401. Go to original source...
    2. Agboola S., Darren N.G., Mills D. (2005): Characterisation and functional properties of Australian rice protein isolates. Journal of Cereal Science, 41: 283-290. Go to original source...
    3. Bera M.B., Mukherjee R.K. (1989): Solubility, emulsifying, and foaming properties of rice bran protein concentrates. Journal of Food Science, 54: 142-145. Go to original source...
    4. Chove B.E., Grandison A.S., Lewis M.J. (2007): Some functional properties of fractionated soy protein isolates obtained by microfiltration. Food Hydrocolloids, 21: 1379-1388. Go to original source...
    5. Damodaran S. (1997): Food proteins. a overview. In: Damodaran S., Paraf A. (eds): Food Proteins and their Applications. 1st Ed. New York, Marcel Dekker: 1-21. Go to original source...
    6. Fabian C.B., Huynh L.H., Ju Y.H. (2010): Precipitation of rice bran protein using carrageenan and alginate. LWT-Food Science and Technology, 43: 375-379. Go to original source...
    7. Garica-Carreno F.L., Dimes L.E., Haard N.F. (1993): Substrate-gel electrophoresis for composition and molecular weight of proteinases or proteinaceous proteinase inhibitors. Analytical Biochemistry, 214: 65-69. Go to original source... Go to PubMed...
    8. German B., Damodatan S., Kinsella J.E. (1982): Thermal dissociation and association behavior of soy proteins. Journal of Agricultural and Food Chemistry, 30: 807-811. Go to original source...
    9. Gnanasambandam R., Heitiarachchy N.S. (1995): Protein concentrates from unstabilized and stabilized rice bran: preparation and properties. Journal of Food Science, 60: 1066-1069. Go to original source...
    10. Hamada J.S. (1997): Characterization of protein fractions of rice bran to devise methods of protein solubilization. Cereal Chemistry, 74: 662-668. Go to original source...
    11. Haskard C.A., Li-Chan E.C.Y. (1998): Hydophobicity of bovine serum albumin and albumin determined using uncharged (PRODAN) and anionic (ANS-) fluorescent probes. Journal of Agricultural and Food Chemistry, 46: 2671-2677. Go to original source...
    12. Jiang S.J., Zhao X.H. (2010): Transglutaminase-induced cross-linking and glucosamine conjugation in soybean protein isolates and its impacts on some functional properties of the products. European Food Research and Technology, 231: 679-689. Go to original source...
    13. Kingsley K., Addob K., Xiong Y.L. (2009): Emulsifying and foaming properties of transglutaminase-treated wheat gluten hydrolysate as influenced by pH, temperature and salt. Food Hydrocolloids, 23: 72-81. Go to original source...
    14. Kinsella J.E. (1982): Functional properties of proteins: Possible relationships between structure and function in foams. Food Chemistry, 7: 273-288. Go to original source...
    15. Laemmli U.K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685. Go to original source... Go to PubMed...
    16. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. (1951): Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193: 265-275. Go to original source...
    17. Mao X., Hua Y. (2012): Composition, structure and functional properties of protein concentrates and isolates produced from Walnut (Juglans regia L.). International Journal of Molecular Science, 13: 1561-1581. Go to original source... Go to PubMed...
    18. Mohamed A., Peterson S.C., Hojilla-Evangelista M.P., Sessa D.J., Rayas-Duarte P., Biresaw G. (2004): Effect of heat treatment and pH on the physicochemical properties of lupin protein. Journal of the American Oil Chemists Society, 81: 1153-1157. Go to original source...
    19. Molina Ortiz S.E., Wagner J.R. (2002): Hydrolysates of native and modified soy protein isolates: structural characteristics, solubility and foaming properties. Food Research International, 35: 511-518. Go to original source...
    20. Moure A., Sineiro J., Dominguez H., Parajo J.C. (2006): Functionality of oilseed protein products: a review. Food Research International, 39: 945-963. Go to original source...
    21. Ogunwolu S.O., Henshaw F.O., Mock H.P., Santros A., Awonorin S.O. (2009): Functional properties of protein concentrates and isolates produced from cashew (Anacardium occidentale L.) nut. Food Chemistry, 115: 852-858. Go to original source...
    22. Osborne T.B. (1907): The Proteins of the Wheat Kernel. Washington, Carnegie Institution of Washington, 84: 5-119. Go to original source...
    23. Pour-Ei A. (1981): Protein functionality: classification, definition and methodology. In: Cherry J.-P: Protein Functionality in Foods. ACS Symposium Series. Washington: American Chemical Society: 1-19. Go to original source...
    24. Shih F.F., Champagne E.T., Daigle K., Zarins Z. (1999): Use of enzymes in the processing of protein products from rice bran and rice flour. Nahrung, 43: 14-18. Go to original source...
    25. Singh N., Kaur M., Sandhu K.S. (2005): Physicochemical and functional properties of freeze-dried and oven dried corn gluten meals. Drying Technology, 23: 975-988. Go to original source...
    26. Snow S.D., Brooks J.R. (1989): Fractionation of rice glutelin polypeptides using gel filtration chromatography. Journal of Food Science, 54: 730-733. Go to original source...
    27. Sze-Tao K.W.C., Sathe S.K. (2000): Functional properties and in vitro digestibility of almond (Prunus dulcis L.) protein isolate. Food Chemistry, 69: 153-160. Go to original source...
    28. Tang C.H., Sun X. (2010): Physicochemical and structural properties of 8S and/or 11S globulins from mungbean [Vigna radiate (L.) Wilczek] with various polypeptide constituents. Journal of Agricultural and Food Chemistry, 258: 6395-6402. Go to original source... Go to PubMed...
    29. Tang S., Hettiarachchy N.S., Shellhammer T.H. (2002): Protein extraction from heat-stabilized defatted rice bran. 1. Physical processing and enzyme treatments. Journal of Agricultural and Food Chemistry, 50: 7444-7448. Go to original source... Go to PubMed...
    30. Wang M., Hettiarachchy N.S., Qi M., Burks W., Seibenmorgen T. (1999): Preparation and functional properties of rice bran protein isolate. Journal of Agricultural and Food Chemistry, 47: 411-416. Go to original source... Go to PubMed...
    31. Wu W., Zhang C., Kong X., Hua Y. (2009): Oxidative modification of soy protein by peroxyl radicals. Food Chemistry, 116: 295-301. Go to original source...
    32. Zhang H.J., Zhang H., Wang L., Guo X.N. (2012): Preparation and functional properties of rice bran proteins from heat-stabilized defatted rice bran. Food Research International, 47: 359-363. Go to original source...

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