Czech J. Food Sci., 2015, 33(2):165-173 | DOI: 10.17221/326/2014-CJFS

Quality control of chondroitin sulphate used in dietary supplementsFood Analysis, Food Quality and Nutrition

Eliška VÁCLAVÍKOVÁ, František KVASNIČKA
Department of Food Preservation, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Prague, Czech Republic

Chondroitin sulphate (CS), a complex polysaccharide isolated from animal cartilage, is used as an ingredient in dietary supplements to support joint health and/or to treat osteoarthritis. Several low-quality CS raw materials were identified in marketed products of dietary supplements. For the evaluation of the quality of CS, we present here a capillary isotachophoretic method (cITP). As a leading electrolyte for the anionic analysis of CS, a mixture of 5 mM HCl + 10 mM glycine + 0.01% of 2-hydroxyethylcellulose of pH 2.8 was used. A solution of 10 mM citric acid served as the terminating electrolyte. On a series of CS raw material samples we proved that the cITP is a suitable method for this purpose.

Keywords: capillary isotachophoresis; electrophoresis

Published: April 30, 2015  Show citation

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VÁCLAVÍKOVÁ E, KVASNIČKA F. Quality control of chondroitin sulphate used in dietary supplements. Czech J. Food Sci. 2015;33(2):165-173. doi: 10.17221/326/2014-CJFS.
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    References

    1. Choi D.W., Kim M.J., Kim H.S., Chang S.H., Jung G.S., Shin K.Y., Chang S.Y. (2003): A size-exclusion HPLC method for determination of chondroitin sulfate in pharmaceutical preparations. Journal of Pharmaceutical and Biomedical Analysis, 31: 1229-1236. Go to original source... Go to PubMed...
    2. Grøndhal F., Tveit H., Akslen-Hoel L.K., Prydz K. (2011): Easy HPLC-based separation and quantitation of chondroitin sulphate and hyaluronan disaccharides after chondroitinase ABC treatment. Carbohydrate Research, 346: 50-57. Go to original source... Go to PubMed...
    3. Grossman P.D., Colburn J.C. (eds) (1992): Capillary Electrophoresis: Theory and Practice. San Diego, Academic Press, Inc.
    4. Grossman P.D., Soane D.S. (1991): Experimental and theoretical studies of DANN separations by capillary electrophoresis in entangled polymer solutions. Biopolymers, 31: 1221-1228. Go to original source... Go to PubMed...
    5. Jin P., Ma J., Wu X., Zou D., Sun C., Hu X. (2009): Simultaneous determination of chondroitin sulfate sodium, allantoin and pyridoxine hydrochloride in pharmaceutical eye drops by an ion-pair high-performance liquid chromatography. Journal of Pharmaceutical and Biomedical Analysis, 50: 293-297. Go to original source... Go to PubMed...
    6. Malavaki C.J., Asimakoupolou A.P., Lamari F.N., Theocharis A.J., Tzanakakis G.N., Karamanos N.K. (2008): Capillary electrophoresis for the quality control of chondroitin sulfates in raw materials and formulations. Analytical Biochemistry, 374: 213-220. Go to original source... Go to PubMed...
    7. Okamota H., Nakajima T., Ito Y., Shimada K., Yamato S. (2004): Development of a novel method for determination of chondroitin sulfate using an in-capillary enzyme reactions. Journal of Chromatography A, 1035: 137-144. Go to original source... Go to PubMed...
    8. Sim J.S., Jun G., Toida T., Cho S.Y., Choi D.W., Chang S.Y., Lindhardt R.J., Kim Y.S. (2005): Quantitative analysis of chondroitin sulfate in raw materials, ophtalmic solutions, soft capsules and liquid preparations. Journal of Chromatography B, 818: 133-139. Go to original source... Go to PubMed...
    9. Tommeraas K., Wahlund P.O. (2009): Poly-acid properties of biosynthetic hyaluronan studied by titration. Carbohydrate Polymers, 77: 194-200. Go to original source...
    10. Václavíková E., Kvasnička F. (2013): Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements. Czech Journal of Food Sciences, 31: 55-65. Go to original source...
    11. Volpi N. (2007): Analytical aspects of pharmaceutical grade chondroitin sulfates. Journal of Pharmaceutical Sciences, 96: 3168-3180. Go to original source... Go to PubMed...
    12. Volpi N. (2009): Quality of different chondroitin sulfate preparations in relations to their therapeutic activity. Journal of Pharmacy and Pharmacology, 61: 1271-1280. Go to original source... Go to PubMed...
    13. Volpi N., Maccari F. (2008): Quantitative and qualitative evaluations of chondroitin sulfate in dietary supplements. Food Analytical Methods, 1: 195-204. Go to original source...
    14. Wang H., Loganathan D., Lindhardt R.J. (1991): Determination of the pKa of glucuronic acid and the carboxy groups of heparin by 13C-nuclear-magnetic-resonance spectroscopy. Biochemistry Journal, 278: 689-695. Go to original source... Go to PubMed...
    15. Yamamoto S., Ohta D., Morikawa Y., Bunseki Kagaku (1982): Determination of sodium chondroitin sulfate in cosmetic products by capillary tube isotachophoresis. Bunseki Kagaku (Jap. Anal.), 31: 251-254. Go to original source...

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