Czech J. Food Sci., 2015, 33(2):148-155 | DOI: 10.17221/365/2014-CJFS

Characterisation of hop varieties grown in Romania based on their contents of bitter acids by HPLC in combination with chemometrics approachFood Analysis, Food Quality and Nutrition

Liana-Claudia SALANȚĂ1, Maria TOFANĂ1, Sonia SOCACI1, Elena MUDURA1, Anca FĂRCAȘ1, Carmen POP1, Anamaria POP1, Antonia ODAGIU2
1 Department of Food Science, Faculty of Food Science and Technology and
2 Department of Environmental Protection and Rural Development, Faculty of Agriculture, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania

Reverse-phase high performance liquid chromatography technique was used for studying the evolution of bitter acids from three varieties of hop growing in Romania during the development of hop cones and the pelletisation process in order to provide the information on the bitter acids profile of each cultivar. Chemometrics methods were applied for highlighting the statistical correlations existing between the genotype (variety), chemotype (composition), and phenotype (phenophase of cone development) with respect to the classes of biologically active compounds investigated (bitter acids). The bitter acid content of each hop cultivar was not only significantly dependent on the phenophases of the cones, but was also influenced by the harvest year. The variations in the α/β ratio as well as cohumulone and colupulone contents were low in both experimental years and the cohumulone/∑α did fraction not exceed 30% in any of the three varieties.

Keywords: α-acids; β-acids; Romanian hop cultivars; Humulus lupulus; principal component analysis

Published: April 30, 2015  Show citation

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SALANȚĂ L, TOFANĂ M, SOCACI S, MUDURA E, FĂRCAȘ A, POP C, et al.. Characterisation of hop varieties grown in Romania based on their contents of bitter acids by HPLC in combination with chemometrics approach. Czech J. Food Sci. 2015;33(2):148-155. doi: 10.17221/365/2014-CJFS.
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References

  1. Anonymous (2006): Analytica EBC (Analityca European Brewery Convention). Methods 7.2, 7.4, 7.7. 6 th Ed. Nürnberg, Verlag Hans Carl Getränke-Fachverlag.
  2. Beatson R.A., Ansell K.A., Graham L.T. (2003): Breeding, development, and characteristics of the hop (Humulus lupulus) cultivar 'Nelson Sauvin'. New Zealand Journal of Crop and Horticultural Science, 31: 303-309. Go to original source...
  3. Ceslová L., Holèapek M., Fidler M., Drčtièková J., Miroslav L. (2009): Characterization of prenylflavonoids and hop bitter acids in various classes of Czech beers and hop extracts using high-performance liquid chromatographymass spectrometry. Journal of Chromatography A, 1216: 7249-7257. Go to original source... Go to PubMed...
  4. De Cooman L., Everaert E., De Keukeleire D. (1998): Quantitative analysis of hop acids, essential oils and flavonoids as a clue to the identification of hop varieties. Phytochemical Analysis, 9: 145-150. Go to original source...
  5. Gad H.A., El-Ahmady S.H., Abou-Shoer M.I., Al-Azizi M.M. (2013): Application of chemometrics in authentication of herbal medicines: a review. Phytochemical Analysis, 24: 1-24. Go to original source... Go to PubMed...
  6. Gerhäuser C. (2005): Beer constituents as potential cancer chemopreventive agents. European Journal of Cancer, 41: 1941-1954. Go to original source... Go to PubMed...
  7. Hoek A.C., Hermans-Lokkerbol A.C.J., Verpoorte R. (2001): An improved NMR method for the quantification of α-acids in hops and hop products. Phytochemical Analysis, 12: 53-57. Go to original source...
  8. Jaskula B., Goiris K., De Rouck G., Aerts G., De Cooman L. (2007): Enhanced quantitative extraction and HPLC determination of hop and beer bitter acids. Journal of the Institute of Brewing, 113: 381-390. Go to original source...
  9. Jelínek L., ©neberger M., Karabín M., Dostálek P. (2010): Comparison of Czech hop cultivars based on their contents of secondary metabolites. Czech Journal of Food Sciences, 28: 309-316. Go to original source...
  10. Khatib A., Wilson E.G., Kim H.K., Supardi M., Choi Y.H., Verpoorte R. (2007): NMR assignment of iso-α-acids from isomerised extracts of Humulus lupulus L. cones. Phytochemical Analysis, 18: 371-377. Go to original source... Go to PubMed...
  11. Krofta K. (2003): Comparison of quality parameters of Czech and foreign hop varieties. Plant, Soil and Environment, 49: 261-268. Go to original source...
  12. Lorenzana A., Hermoso de Mendoza A., Seco M.V., Casquero P.A. (2010): Population development of Phorodon humuli and predators (Orius spp.) within hop cones: influence of aphid density on hop quality. Crop Protection, 29: 832-837. Go to original source...
  13. Mitter W., Steiner S.H., Hopfen G.M., Mainburg B.H. (2009): Annual fluctuations in hop quality - options for adjustment in the brewhouse. Brauwelt International, 2009/I: 36-37.
  14. Negri G., Santi D., Tabach R. (2010). Bitter acids from hydroethanolic extracts of Humulus lupulus L., Cannabaceae, used as anxiolytic. Revista Brasileira de Farmacognosia Brazilian Journal of Pharmacognosy, 20: 850-859. Go to original source...
  15. Patzak J., Nesvadba V., Henychová A., Krofta K. (2010): Assessment of the genetic diversity of wild hops (Humulus lupulus L.) in Europe using chemical and molecular analyses. Biochemical Systematics and Ecology, 38: 136-145. Go to original source...
  16. Salanță L.C., Tofană M., Socaci S., Mudura E., Fărcaș A. (2013): The internal validation of HPLC and conductometric method for the determination of hop bitter acids. Journal of Agroalimentary Processes and Technologies, 19: 362-366.
  17. Sahlstrom A., Rostad S. (2011): HPLC determination of α- and β-acids in hops. Concordia College Journal of Analytical Chemistry, 2: 78-83.
  18. Shellie R.A., Poynter S.D.H., Gathercole J.L., Whittock S.P., Koutoulis A. (2009): Varietal characterization of hop (Humulus lupulus L.) by GC-MS analysis of hop cone extracts. Journal of Separation Science, 32: 3720-3725. Go to original source... Go to PubMed...
  19. Sreèec S., Zechner-Krpan V., Petraviæ-Tominac V., Èerenak A., Liber Z., ©atoviæ Z. (2010): Phenotypic and alpha-acid content diversity of wild hop populations in Croatia. Plant, Soil and Environment, 56: 37-42. Go to original source...
  20. Tofană M., Mora A., Socaci S., Muste S., Mudura E., Michiu D., Salanță L., Fărcaș A. (2012): The content in alpha bitter acids of hop cultivars cultivated in Romania during 2008-2011. Hop and Medicinal Plants, 20: 23-28. Go to original source...
  21. Tofană M. (2006): Substanțe amare și de aromă din hamei. Cluj-Napoca, Ed. Alma Mater: 23-24.
  22. Van Cleemput M., Cattoor K., De Bosscher K., Haegeman G., De Keukeleire D., Heyerick A. (2009): Hop (Humulus lupulus)-derived bitter acids as multipotent bioactive compounds. Journal of Natural Products, 72: 1220-1230. Go to original source... Go to PubMed...
  23. Zhang X., Liang X., Xiao H., Xu Q. (2004): Direct characterization of bitter acids in a crude hop extract by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry. Journal of the American Society for Mass Spectrometry, 15: 180-187. Go to original source... Go to PubMed...

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