Czech J. Food Sci., 2015, 33(1):77-82 | DOI: 10.17221/498/2013-CJFS

Determination of fluoride in Antarctic krill (Euphausia superba) using ion chromatography and its pretreatments selectionFood Analysis, Food Quality and Nutrition

Yan-Ling ZHAO^1,2, Lan-Lan ZHU¹, Yong SUN¹, De-Qing ZHOU¹

¹ Department of Food Engineering and Nutrition, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong Province, P.R. China

² College of Food Science and Technology, Shanghai Ocean University, Shanghai, P.R. China

A rapid, sensitive and reliable method to quantify fluoride in Antarctic krill has been established. Four different pretreatment methods were used for the extraction of fluoride: double-deionised water extraction, sulphuric acid distillation, hydrochloric acid extraction, and pH adjustment with buffer after hydrochloric acid extraction. Four methods of comparative analysis revealed that sulphuric acid distillation was suitable preparation for ion chromatography determination of fluoride in Antarctic krill (fluoride content 288.7 ± 10.2 mg/kg). The method was partially validated in linearity, accuracy, and precision. The linear range was from 0.1 to 10.0 mg/l with the regression coefficient of 0.99998. The accuracy expressed as the recoveries of standard addition ranged from 95.3% to 101.3%, the relative standard deviation (n = 8) was 1.8-1.9%. With this method, the 3σ limit of detection was 0.06 mg/l of fluoride in Antarctic krill. Our results indicate that the method (limit of quantification 0.2 mg/l) could be well applied for the determination of fluoride in Antarctic krill.

Keywords: fluoride in krill; fluoride quantification; extraction methods; preparations

Published: February 28, 2015  Show citation