Czech J. Food Sci., 2015, 33(1):52-57 | DOI: 10.17221/198/2014-CJFS

Evaluation of patulin in commercial baby foods by solid phase extraction and liquid chromatography PDA detectionFood Analysis, Food Quality and Nutrition

Arslan KARAKOSE1, Senem SANLI2, Nurullah SANLI2, Ibrahim BULDUK2
1 Turkish Standards Institution, Çorum Regional Directorate, Çorum, Turkey
2 Department of Chemistry, Faculty of Science and Arts, Uºak University, Uºak, Turkey

A simple, reliable, and rapid RP-LC method has been developed for the determination of patulin in commercial baby foods from Turkish markets. Solid phase extraction and liquid chromatography with photodiode array detection technique were used for the analysis of patulin in samples. Linearity was obtained in different concentration ranges between 0.001 and 0.050 µg/l. The recovery rates for patulin ranged from 92.85% to 100.45% with a coefficient of variation less than 3.0%. LOD and LOQ of the method were found to be 9.66 × 10-6 and 2.93 × 10-5 µg/ml, respectively. The proposed method has been extensively validated in accordance with ICH guidelines. It can be used by routine analysis laboratories within a short time of analysis. All of the detected samples containing patulin were below the level suggested by the World Health Organization.

Keywords: homogenised puree; HPLC; patulin; Turkish markers; SPE

Published: February 28, 2015  Show citation

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KARAKOSE A, SANLI S, SANLI N, BULDUK I. Evaluation of patulin in commercial baby foods by solid phase extraction and liquid chromatography PDA detection. Czech J. Food Sci. 2015;33(1):52-57. doi: 10.17221/198/2014-CJFS.
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    References

    1. Alfonso F.C., Martin G.E, Randolph H.D. (1980): Highpressure liquid chromatographic determination of 5-hydroxymethyl-2-furaldehyde in caramel solution. Journal of Association of Official Analytical Chemists, 63: 1310. Go to original source...
    2. Bonerba E., Conte R., Ceci E., Tantillo G., (2010): Assessment of dietary intake of patulin from baby foods. Journal of Food Science, 75: T123-T125. Go to original source... Go to PubMed...
    3. Brause A.R., Trucksess M.W., Thomas F.S., Page S.W., (1996): Determination of patulin in apple juice by liquid chromatography: collaborative study. Journal of AOAC International, 79: 451-455. Go to original source... Go to PubMed...
    4. Commission Regulation (EC) No. 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Union, L 364/5-23.
    5. European Standard Report 15890 EN (2010): Foodstuffs - Determination of patulin in fruit juice and fruit based purée for infants and young children - HPLC method with liquid/liquid partition cleanup and solid phase extraction and UV detection.
    6. Fuchs S., Sontag G., Stidl R., Ehrlich V., Kundi M., Knasmuller S. (2008): Detoxification of patulin and ochratoxin A, two abundant mycotoxins by lactic acid bacteria. Food Chemical Toxicology, 46: 1398-1407. Go to original source... Go to PubMed...
    7. Gokmen V., Acar J. (1996): Rapid reversed phase liquid chromatographic determination of patulin in apple juices. Journal of Chromatography A, 730: 53. Go to original source... Go to PubMed...
    8. Gokmen V., Acar J. (1999): Simultaneous determination of 5-hydroxymethylfurfural and patulin in apple juice by reversed-phase liquid chromatography. Journal of Chromatography A, 847: 69-74. Go to original source... Go to PubMed...
    9. Hopkins J. (1993): The toxicological hazards of patulin. Food Chemical Toxicology, 31: 455-456. Go to original source... Go to PubMed...
    10. IARC (1993): IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. Vol. 56: Some Naturally Cccurring Substances: Food Items and Constituents, Heterocyclic Aromatic Amines and Mycotoxins. Vol. 56. Lyon: 489-521.
    11. International Conference on Harmonization (ICH) (2005): Q2(R1) Validation of Analytical Procedure: Text and Methodology. Geneva. 1-13.
    12. Koach J.A.G., White K.D., Trucksess M.W., Thomas F.S. (2000): Capillary gas chromatography/mass spectrometry with chemical ionization and negative ion detection for confirmation of identity of patulin in apple juice. Journal of AOAC International, 83: 104-112. Go to original source... Go to PubMed...
    13. Lee H.S., Roussef R.L, Nagy S. (1986): HPLC determination of furfural and 5-hydroxymethylfurfural in citrus juices. Journal of Food Science, 51: 1075. Go to original source...
    14. Llovera M., Balcells M., Torres M., Canela R. (2005): Parallel synthesis: a new approach for developing analytical internal standards. application to the analysis of patulin by gas chromatography-mass spectrometry. Journal of Agricultural and Food Chemistry, 53: 6643-6648. Go to original source... Go to PubMed...
    15. Pittet A. (2001): Natural occurrence of mycotoxins in foods and feeds: a decade in review. In: Mycotoxins and Phycotoxins in Perspective at the Turn of the Millennium. Proceedings of the Xth International IUPAC Symposium on Mycotoxins and Phycotoxins, 21-25 May, 2000, Guaruja, Brazil, 6: 153-172.
    16. Riley C.M., Rosanske T.W. (1996): Development and Validation of Analytical Methods. New York, Elsevier: 5-69.
    17. Rizzi A., Katz E., Eksteen R., Schoenmakers P.J., Miller, N. (1998): Handbook of HPLC. Chromatographicc Science Series, Volume 78, Chapter 1. New York, Marcel Dekker.
    18. Shepard G.S., Leggott N.L. (2000): Chromatographic determination of the mycotoxin patulin in fruit and fruit juices. Journal of Chromatography A, 882: 17-22. Go to original source... Go to PubMed...
    19. Swartz M.E., Krull I.S. (1997): Analytical Development and Validation. New York, Marcel Dekker: 8-69.
    20. World Health Organization (WHO) (1995): 44th Report of the joint FAO/WHO expert committee on food additives. In Technical Report Series No. 859. Geneva. JECFA: 36-41.

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