Czech J. Food Sci., 2015, 33(1):27-31 | DOI: 10.17221/267/2014-CJFS

Species differentiation of thermotolerant Campylobacters based on distinctive banding patterns obtained by multiplex PCRFood Microbiology and Safety

Lucie VONDRÁKOVÁ, Sabina PURKRTOVÁ, Jarmila PAZLAROVÁ, Kateřina DEMNEROVÁ
Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Prague, Czech Republic

The differentiation of thermotolerant Campylobacter spp. on the species level (C. jejuni, C. coli, C. lari, and C. upsalien-sis) was provided. Identification is based on different banding patterns obtained for individual species during simple multiplex PCR where regions within the 23S rRNA gene are amplified using newly designed specific forward primers.

Keywords: Campylobacteriosis; alimentary infection; 23S rRNA

Published: February 28, 2015  Show citation

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VONDRÁKOVÁ L, PURKRTOVÁ S, PAZLAROVÁ J, DEMNEROVÁ K. Species differentiation of thermotolerant Campylobacters based on distinctive banding patterns obtained by multiplex PCR. Czech J. Food Sci. 2015;33(1):27-31. doi: 10.17221/267/2014-CJFS.
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