Czech J. Food Sci., 2014, 32(5):485-492 | DOI: 10.17221/415/2013-CJFS

The impact of moist corn grain preservation on the ethanol yield by simultaneous saccharification and fermentation, and on volatile by-productsOriginal Paper

Jacek NOWAK1, Katarzyna SZAMBELAN1, W³odzimierz NOWAK2
1 Institute of Food Technology of Plant Origin, Faculty of Food Science and Nutrition and
2 Department of Animal Nutrition and Feed Management, Faculty of Animal Breeding and Biology, Poznañ University of Life Sciences, Poznañ, Poland

We assessed the composition of volatile by-products in raw spirits obtained from moist corn fermentation. The average moisture value of the researched samples was 35.4%. A comparative research was conducted applying simultaneous saccharification and fermentation (SSF) process with Saccharomyces cerevisiae. The analysis characterised corn grains after three and six months of storage in aerobic and anaerobic conditions. The yield of ethanol fermentation was 42.43 and 39.12 l/100 kg dry matter after three and six months of storage, respectively. The storage of moist grain resulted in the reduction of higher alcohols content in the raw spirits. It was observed that the esters concentration decreased after three, but increased after six months of raw material storage. A significant increase occurred in the quantity of aldehydes detected only after three months of the corn storage. The results show that the application of SSF technology to moist corn, allows the production of bioethanol with quality comparable to that obtained with dried grain.

Keywords: bioethanol; chemical preservation; SSF; volatile compounds

Published: October 31, 2014  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
NOWAK J, SZAMBELAN K, NOWAK W. The impact of moist corn grain preservation on the ethanol yield by simultaneous saccharification and fermentation, and on volatile by-products. Czech J. Food Sci. 2014;32(5):485-492. doi: 10.17221/415/2013-CJFS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Aksu T., Baytok E., Bolat D. (2004): Effects of a bacterial silage inoculant on corn silage fermentation and nutrient digestibility. Small Ruminant Research, 55: 249-252. Go to original source...
    2. Belyea R.L., Rausch K.D., Tumbleson M.E. (2004): Composition of corn and distillers dried grains with solubles from dry grind ethanol processing. Bioresource Technology, 94: 293-298. Go to original source... Go to PubMed...
    3. Bia³as W., Szymanowska D., Grajek W. (2010): Fuel ethanol production from granular corn starch using Saccharomyces cerevisiae in a long term repeated SSF process with full stillage recycling. Bioresource Technology, 101: 3126-3131. Go to original source... Go to PubMed...
    4. Devantier R., Pedersen S., Olsson L. (2005): Characterisation of very high gravity ethanol fermentation of corm mash. Effect of glucoamylase dosage, presaccharification and yeast strain. Applied Microbiology and Biotechnology, 68: 622-629. Go to original source... Go to PubMed...
    5. GOJ T. (1990): Carbonyl compounds in the raw and rectified spirits of different origin of raw materials and their effect on the sensory properties. Part 1. Carbonyl compounds in raw spirits. Fermentation of Fruits and Vegetable Industry, 8-9: 6-9.
    6. Gumienna M., Lasik M., Czarnecki Z., Szambelan K. (2009): Applicability of unconventional energy raw material in ethanol production. ACTA Scientiarum Polonorum Technologia Alimentaria, 8 (4): 17-24.
    7. Gumienna M., Lasik M., Szambelan K., Czarnecki Z. (2011): Reduction of water consumption in bioethanol production from triticale by recycling the stillage liquid phase. ACTA Scientiarum Polonorum Technologia Alimentaria, 10 (4): 467-474.
    8. Hamelinck C.N., Van Hooijdonk G., Faaij A.P.C. (2005): Ethanol from lignocellulosic biomass: Techno-economic performance in short-, middle-, and long-term. Biomass and Bioenergy, 28: 384-410. Go to original source...
    9. Holm J., Bjõrck I., Drews A. (1986): A rapid method for the analysis of starch. Starch-Stärke, 38: 224-226. Go to original source...
    10. K³osowski G., Mikulski D. (2010): The effect of raw material contamination with mycotoxins on the composition of alcoholic fermentation volatile by-products in raw spirits. Bioresource Technology, 101: 9723-9727. Go to original source... Go to PubMed...
    11. Kupczyk A. (2007): Present state and perspectives of transport biofuel utilisation on the EC background. Energetic and Ecology. Available at www.e-energetyka.pl
    12. Kwiatkowski J.R., McAloon A.J., Taylor F., Johnston D.B. (2006): Modelling the process and costs of fuel ethanol production by the corn dry-grind process. Industrial Crops and Products, 23: 288-296. Go to original source...
    13. Lipski Z. (2002): Corn: Grain Production and CCM. Publisher of Institute of Cultivation, Fertilization and Soil, Pu³awy.
    14. Marin S., Magan N., Abellana M., Canela R., Ramos A.J., Sanchis V. (2000): Selective effect of propionates and water activity on maize mycoflora and impact on fumonisin B1 accumulation. Journal of Stored Products Research, 36: 203-214. Go to original source...
    15. Miecznikowski A.H., Zieliñska K.J. (2002): Effects of selected process steps on the quality of raw spirit. In: Stecka K.M. (ed.): New Trends in Distillery Technology. IBPRS, Warsaw: 109-116.
    16. Miller G. (1959): Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31: 426-428. Go to original source...
    17. Nikoliæ S., Mojoviæ L., Rakin M., Pejin J., DjukiæVukoviæ A., Bulatoviæ M. (2012): Simultaneous enzymatic saccharification and fermentation (SSF) in bioethanol production from corn meal by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus. Journal of Chemical Science and Technology, 1: 23-28.
    18. Nowak J., Szambelan K., Miettinen H., Nowak W., Czarnecki Z. (2008): Effect of the corn grain storage method on saccharification and ethanol fermentation yield. ACTA Scientiarum Polonorum Technologia Alimentaria, 7 (1): 19-27.
    19. Roy S., Gudi R.D., Venkatesh K.V., Shah S.S. (2001): Optimal control strategies for simultaneous saccharification and fermentation of starch. Process Biochemistry, 36: 713-722. Go to original source...
    20. Schmidt R.H., Davidson S.M., Bates R.P. (1983): Acetaldehyde determination in fermented food by direct 2,4-dinitrophenylohydrazone by high-performance liquid chromatography. Journal of Food Science, 48: 1556-1557. Go to original source...
    21. Semencenko V., Mojovic L., Petrovic S., Ocic O. (2011): Recent trends in bioethanol production. Chemical Industry, 65: 103-114. Go to original source...
    22. S³omiñska L., Wi¶niewska D., Grze¶kowiak A. (2003): Liquefaction of starch by thermostabile alpha-amylase. ACTA Scientiarum Polonorum Technologia Alimentaria, 2 (2): 17-26.
    23. Stanisz M., Sapiñska E., Pielech-Przybylska K. (2009): Characterization of contaminants present in raw spirits. Scientific Books of Technical University of £ód¼, Food Chemistry and Biotechnology, No. 1058, Book 73: 105-121.
    24. Szambelan K., Nowak J., Jeleñ H. (2005): The composition of Jerusalem artichoke (Helianthus tuberosus L.) spirits obtained from fermentation with bacteria and yeasts. Engineering in Life Sciences, 5: 68-71. Go to original source...
    25. Wang P., Singh V., Xue H., Johnston D.B., Rausch K.D., Tumbleson M.E. (2007): Comparison of raw starch hydrolyzing enzyme with conventional liquefaction and saccharification enzymes in dry-grind corn processing. Cereal Chemistry, 84: 10-14. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content