Czech J. Food Sci., 2014, 32(5):449-455 | DOI: 10.17221/16/2014-CJFS

A novel sorbent for solid-phase extraction coupling to high performance liquid chromatography for the determination of olaquindox in fish feedOriginal Paper

Dongyan ZHAO1,2, Qiu Yi SHI1, Xiong HE1, Jing ZHANG1
1 Department of Biological and Food, Zhejiang Pharmaceutical College, Ningbo, P.R. China
2 Department of Food Science and Engineering, Shandong Agricultural University, Taian, P.R. China

A new and hydrophilic molecularly imprinted polymer (MIP) selective for olaquindox was prepared by bulk polymerisation using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the crosslinker. The synthesised polymer was characterised by Fourier-transform infrared and static adsorption experiments. Results showed that the MIP had good recognition and selective ability for olaquindox. A novel method based on molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography was developed for the separation and determination of trace olaquindox in feed samples. Under selected experimental conditions, the detection limit (signal-to-noise ratio = 3) was 42.2 ng/g, and the relative standard deviation (RSD) for five replicate extractions of 50 µg/l olaquindox was 4.9%. The method provided high recoveries ranging from 89.8% to 93.1% at three spiked levels with < 5% RSDs. This method was successfully applied for the analysis of olaquindox in feed.

Keywords: HPCL; molecular imprinting; SPE; olaquindox

Published: October 31, 2014  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
ZHAO D, SHI QY, Xiong H, ZHANG J. A novel sorbent for solid-phase extraction coupling to high performance liquid chromatography for the determination of olaquindox in fish feed. Czech J. Food Sci. 2014;32(5):449-455. doi: 10.17221/16/2014-CJFS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Andersson L.I. (2000): Molecular imprinting for drug bioanalysis: A review on the application of imprinted polymers to solid-phase extraction and binding assay. Journal of Chromatography B, 739: 163-173. Go to original source...
    2. Carta A., Corona P., Loriga M. (2005): Quinoxaline 1,4-dioxide: A versatile scaffold endowed with manifold activities. Current Medicinal Chemistry, 12: 2259-2272. Go to original source... Go to PubMed...
    3. Ding M.X., Wang Y.L., Zhu H.L., Yuan Z.H. (2006): Effects of cyadox and olaquindox on intestinal mucosal immunity and on fecal shedding of Escherichia coli in piglets. Journal of Animal Science, 84: 2367-2373. Go to original source... Go to PubMed...
    4. Francesco P., Carmelo G., Francesca I., Rita M., Umile G.S., Nevio P. (2005): Molecularly imprinted solid phase extraction for detection of Sudan I in food matrices. Food Chemistry, 93: 349-353. Go to original source...
    5. Gizzi G., Vincent U., Holst C.V., Jong J.D., Genouel C. (2007): Validation of an analytical method for the determination of carbadox and olaquindox in feedstuff by liquid chromatography coupled to UV and/or diode array detection. Food Additives and Contaminants, 24: 1226-1235. Go to original source... Go to PubMed...
    6. Hao L.H., Chen Q., Xiao X.L. (2006): Molecular mechanism of mutagenesis induced by olaquindox using a shuttle vector pSP189/mammalian cell system. Mutation Research/ Fundamental and Molecular Mechanisms of Mutagenesis, 599: 21-25. Go to original source... Go to PubMed...
    7. Haupt K., Dzgoev A., Mosbach K. (1998): Assay system for the herbicide 2, 4-dichlorophenoxyacetic acid using a molecularly imprinted polymer as an artificial recognition element. Analytical Chemistry, 70: 628-631. Go to original source... Go to PubMed...
    8. Hutchinson M.J., Young P.B., Kennedy D.G. (2005): Confirmatory method for the analysis of carbadox and olaquindox in porcine feeding stuffs using LC-electrospray MS-MS. Food Additives and Contaminants, 22: 113-119. Go to original source... Go to PubMed...
    9. Lin S.Y., Jeng S.L. (2001): High-performance liquid chromatographic determination of carbadox, olaquindox, furazolidone, nitrofurazone, and nitrovin in feed. Journal of Food Protection, 64: 1231-1234. Go to original source... Go to PubMed...
    10. Nasr J.J., Shalan Sh., Belal F. (2013): Determination of carbadox and olaquindox residues in chicken muscles, chicken liver, bovine meat, liver and milk by MLC with UV detection: Application to Baby Formulae. Chromatographia, 76: 523-528. Go to original source...
    11. Neumann N.J., Blotz A., Wasinska-Kempk G., Rosenbruch M., Lehmann P., Ahr H.J. (2005): Evaluation of phototoxic and photoallergic potentials of 13 compounds by different in vitro and in vivo methods. Journal of Photochemistry and Photobiology B: Biology, 79: 25-34. Go to original source... Go to PubMed...
    12. Poucke C.V., Keyser K.D., Baltusnikiene A., McEvoy J.D.G., Peteghema C.V. (2003): Liquid chromatographictandem mass spectrometric detection of banned antibacterial growth promoters in animal feed. Analytica Chimica Acta, 483: 99-109. Go to original source...
    13. Roets E., Quintens L., Kibaya R., Hoogmartens J. (2001): Quantitative determination of olaquindox in animal feed. Journal of Planar Chromatography - Modern TLC, 14: 347-349.
    14. Sellergren B. (1994): Direct drug determination by selective sample enrichment on an imprinted polymer. Analytical Chemistry, 66: 1578-158. Go to original source...
    15. Snyder L.R., Kirkland J.J., Glajch J.L. (1997): Quantitation (including trace analysis). In: Practical HPLC Method Development. John Wiley & Sons, Inc; New York: 643-684. Go to original source...
    16. Song J., Qiao X., Chen H., Zhao D., Zhang Y., Xu Z. (2011): Molecularly imprinted solid-phase extraction combined with high-performance liquid chromatography for analysis of trace olaquindox residues in chick feeds. Journal of the Science of Food and Agriculture, 91: 2378-2385. Go to original source... Go to PubMed...
    17. Wang K., Geng Y. (2002): Pathological study on the acute olaquindox poisoning in carp. Acta Veterinaria et Zootechnica Sinica, 6: 565-569.
    18. Wang K., Geng Y. (2003): Studies on haematological changes in common carps by subacute toxicity test of olaquindox. Acta Hydrobiologica Sinica, 1: 23-26.
    19. Wu Y.J., Wang Y.L., Huang L.L., Tao Y.F., Yuan Z.H., Chen D.M. (2006): Simultaneous determination of five quinoxaline-1,4-dioxides in animal feeds using ultrasonic solvent extraction and high performance liquid chromatography. Analytica Chimica Acta, 569: 97-102. Go to original source...
    20. Xu Z.X., Song J.M., Li L.Q., Qiao X.G., Chen H.H. (2012): Development of an on-line molecularly imprinted chemiluminescence sensor for determination of trace olaquindox in chick feeds. Journal of the Science of Food and Agriculture, 92: 2696-2702. Go to original source... Go to PubMed...
    21. Zhang H.Y., Wei Y.W., Zhou J.H., Xu Z.X., Tian S., He J.X. (2013): Preparation and application of a molecular imprinting matrix solid phase dispersion extraction for the determination of olaquindox in chicken by high performance liquid chromatography. Food Analytical Methods, 6: 915-921. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content