Czech J. Food Sci., 2014, 32(4):337-341 | DOI: 10.17221/421/2013-CJFS

Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy productsOriginal Paper

Alžbeta Medveďová, Adriana Studeničová, Ľubomír Valík, Zuzana Horváthová
Department of Nutrition and Food Assessment, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovak Republic

We investigated the prevalence of enterotoxigenic S. aureus in the Slovakian dairy products and compared the reliability of different routine methods of identification. Out of 64 isolates, 44% were confirmed as S. aureus. There was only a little correlation in the confirmation by API Staph, VIT-Staphylococcus, and PCR detection. The PCR results revealed that 32% of S. aureus isolates possessed the selected gene for SEs enterotoxins (SEA-SEE), with sea as the most predominant gene. Neither seb nor sed genes were found throughout the collection. Additionally, the growth analysis of the isolates was performed in milk at 15°C. The growth parameters were very close to one another and were also compared with the data described in the global databases. These results provided evidence that the growth rate and lag phase duration can be determined with a high degree of reproducibility without regardless of the strain origin.

Keywords: coagulaso-positive staphylococci; staphylococcal enterotoxins; PCR; growth dynamic

Published: August 31, 2014  Show citation

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Medveďová A, Studeničová A, Valík Ľ, Horváthová Z. Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy products. Czech J. Food Sci. 2014;32(4):337-341. doi: 10.17221/421/2013-CJFS.
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