Czech J. Food Sci., 2014, 32(4):326-336 | DOI: 10.17221/472/2013-CJFS

Enzymatically hydrolysed molasses and sodium citrate as new potentials for the improvement of canthaxanthin batch synthesis by Dietzia natronolimnaea HS-1: A statistical media optimisationOriginal Paper

Seyed Mohammad Taghi Gharibzahedi, Seyed Hadi Razavi, Mohammad Mousavi
Center of Excellence for Application of Modern Technologies for Producing Functional Foods and Drinks and Bioprocess Engineering Laboratory (BPEL), Department of Food Science, Engineering & Technology, Faculty of Agricultural Engineering and Technology, University of Tehran, Karaj, Iran

Response surface methodology-central composite rotatable design (RSM-CCRD) was applied to explore the optimum media formulation for maximising canthaxanthin (CTX) biosynthesis by Dietzia natronolimnaea HS-1. The effects of three variables of enzymatically hydrolysed molasses (EHM) (16.6-33.4 g/l), sodium (Na)-citrate (21.64-28.36mM), and yeast extract (6.32-9.68 g/l) concentrations on the production of CTX, total carotenoid (TCT), and biomass dry weight (BDW) were appraised. The results showed that the quadratic effects of EHM, yeast extract, and Na-citrate contents in terms of second-order polynomial regression equations (R2 = 0.968-0.986), respectively, had the most significant effects on the produced TCT and CTX. The predicted maximum accumulation of BDW (8.88 g/l), TCT (7.24 mg/l), and CTX (6.40 mg/l) under the optimum concentrations of the media variables (26.16 g/l EHM, 8.29 g/l yeast extract, and 25.86mM Na-citrate) was very close to the experimental values determined in batch experiments. The high BDW content suggested EHM and Na-citrate as very promising feedstocks for CTX bioproduction by the bacterium studied.

Keywords: enzymatic hydrolysis; sugarbeet molasses hydrolysate; carotenoid pigment; batch submerged fermentation; response surface modelling

Published: August 31, 2014  Show citation

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Gharibzahedi SMT, Razavi SH, Mousavi M. Enzymatically hydrolysed molasses and sodium citrate as new potentials for the improvement of canthaxanthin batch synthesis by Dietzia natronolimnaea HS-1: A statistical media optimisation. Czech J. Food Sci. 2014;32(4):326-336. doi: 10.17221/472/2013-CJFS.
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    References

    1. Avci F.G., Huccetogullari D., Azbar N. (2014): The effects of cell recycling on the production of 1,3-propanediol by Klebsiella pneumonia. Bioprocess and Biosystems Engineering, 37: 513-519. Go to original source... Go to PubMed...
    2. Bhosale P. (2004): Environmental and cultural stimulants in the production of carotenoids from microorganisms. Applied Microbiology and Biotechnology, 63: 351-361. Go to original source... Go to PubMed...
    3. Bhosale P., Gadre R.V. (2002): Manipulation of temperature and illumination conditions for enhanced β-carotene production by mutant 32 of Rhodotorula glutinis. Letters in Applied Microbiology, 34: 349-353. Go to original source... Go to PubMed...
    4. Bhosale P.B., Gadre R.V. (2001): Production of β-carotene by a mutant of Rhodotorula glutinis. Applied Microbiology and Biotechnology, 55: 423-427. Go to original source... Go to PubMed...
    5. Bouallagui H., Touhami Y., Hanafi N., Ghariani A., Hamdi M. (2013): Performances comparison between three technologies for continuous ethanol production from molasses. Biomass and Bioenergy, 48: 25-32. Go to original source...
    6. Boulton C.A., Ratledge C. (1981): Correlation of lipid accumulation in yeast with possession of ATP:citrate lyase. Journal of General Microbiology, 127: 169-176. Go to original source...
    7. Evans C.T., Scragg A.H., Ratledge C. (1983): Regulation of citrate efflux from mitochondria of oleaginous and non oleaginous yeast by adenine nucleotides. European Journal of Biochemistry, 132: 609-615. Go to original source... Go to PubMed...
    8. Flores-Cotera L.B., Martin R., Sanchez S. (2001): Citrate, a possible precursor of astaxanthin in Phaffia rhodozyma: influence of varying levels of ammonium, phosphate and citrate in a chemically defined medium. Applied Microbiology and Biotechnology, 55: 341-347. Go to original source... Go to PubMed...
    9. Gaudreau H., Champagne C.P., Goulet J., Conway J. (1997): Lactic fermentation of media containing high concentrations of yeast extracts. Journal of Food Science, 62: 1072-1075. Go to original source...
    10. Gharibzahedi S.M.T., Razavi S.H., Mousavi S.M., Moayedi V. (2012a): High efficiency canthaxanthin production by a novel mutant isolated from Dietzia natronolimnaea HS-1 using central composite design analysis. Industrial Crops and Products, 40: 345-354. Go to original source...
    11. Gharibzahedi S.M.T., Razavi S.H., Mousavi S.M. (2012b): Developing an emulsion model system containing canthaxanthin biosynthesized by Dietzia natronolimnaea HS-1. International Journal of Biological Macromolecules, 51: 618-626. Go to original source... Go to PubMed...
    12. Gharibzahedi S.M.T., Razavi S.H., Mousavi S.M. (2013a): Comparison of antioxidant and free radical scavenging activities of biocolourant synthesized by Dietzia natronolimnaea HS-1 cells grown in batch, fed-batch and continuous cultures. Industrial Crops and Products, 49: 10-16. Go to original source...
    13. Gharibzahedi S.M.T., Razavi S.H., Mousavi S.M. (2013b): Microbial canthaxanthin: Perspectives on biochemistry and biotechnological production. Engineering in Life Sciences, 13: 408-417. Go to original source...
    14. Gharibzahedi S.M.T., Razavi S.H., Mousavi S.M. (2013c): Psyllium husk gum: An attractive carbohydrate biopolymer for the production of stable canthaxanthin emulsions. Carbohydrate Polymers, 92: 2002-2011. Go to original source... Go to PubMed...
    15. Gharibzahedi S.M.T., Razavi S.H., Mousavi S.M. (2014): Potential applications and emerging trends of species of the genus Dietzia: a review. Annals of Microbiology, 64: 421-429. Go to original source...
    16. Ghasemlou M., Khodaiyan F., Gharibzahedi S.M.T. (2012): Enhanced production of Iranian kefir grain biomass by optimization and empirical modeling of fermentation conditions using response surface methodology. Food and Bioprocess Technology, 5: 3230-3235. Go to original source...
    17. Goksungur Y., Mantzouridou F., Roukas T. (2002): Optimization of the production of β-carotene from molasses by Blakeslea trispora: a statistical approach. Journal of Chemical Technology and Biotechnology, 77: 933-943. Go to original source...
    18. Goksungur Y., Mantzouridou F., Roukas T., Kotzekidou P. (2004): Production of β-carotene from beet molasses by Blakeslea trispora in stirred-tank and bubble column reactors. Applied Biochemistry and Biotechnology, 112: 37-54. Go to original source... Go to PubMed...
    19. Janssen M., Winther M., Tramper J. (2000): Efficiency of light utilization of Chlamydomonas reinhardtii under medium light/dark cycles. Journal of Biotechnology, 78: 123-137. Go to original source... Go to PubMed...
    20. Khodaiyan F., Razavi S.H., Emam-Djomeh Z., Mousavi S.M.A., Hejazi M.A. (2007): Effect of culture conditions on canthaxanthin production by Dietzia natronolimnaea HS-1. Journal of Microbiology and Biotechnology, 17: 195-201.
    21. Khodaiyan F., Razavi S.H., Mousavi S.M. (2008): Optimization of canthaxanthin production by Dietzia natronolimnaea HS-1 from cheese whey using statistical experimental methods. Biochemical Engineering Journal, 40: 415-422. Go to original source...
    22. Kim S.J., Kim G.J., Park D.H., Ryu Y.W. (2003): High-level production of astaxanthin by fed-batch culture of mutant strain Phaffia rhodozyma AJ-6-1. Journal of Microbiology and Biotechnology, 13: 175-181.
    23. Kim J.H., Kang S.W., Kim S.W., Chang H.I. (2005): Highlevel production of astaxanthin by Xanthophyllomyces dendrorhous mutant JH1 using statistical experimental designs. Bioscience, Biotechnology, and Biochemistry, 69: 1743-1748. Go to original source... Go to PubMed...
    24. Kim J.H., Choi S.K., Park Y.S., Yun C.W., cho W.D., Chee K.M., Chang H.I. (2006): Effect of culture conditions on astaxanthin formation in red yeast Xanthophyllomyces dendrorhous mutant JH1. Journal of Microbiology and Biotechnology, 16: 438-442.
    25. Li H.B., Fan K.W., Chen F. (2006): Isolation and purification of canthaxanthin from the microalga Chlorella zofingiensis by high speed counter-current chromatography. Journal of Separation Science, 29: 699-703. Go to original source... Go to PubMed...
    26. Mahro B., Timm M. (2007): Potential of biowaste from the food industry as a biomass resource. Engineering in Life Sciences, 7: 457-468. Go to original source...
    27. Masetto A., Flores-Cotera L.B., Diaz C., Langley E., Sanchez S. (2001): Application of a complete factorial design for the production of zeaxanthin by Flavobacterium sp. Journal of Bioscience and Bioengineering, 92: 55-58. Go to original source...
    28. Miller G.L. (1959): Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31: 426-428. Go to original source...
    29. Myers R.H., Montgomery R.C. (2002): Response Surface Methodology, Process and Product Optimization using Design Experiment. John Wiley & Sons, New York.
    30. Nasri Nasrabadi M.R., Razavi S.H. (2010a): Enhancement of canthaxanthin production from Dietzia natronolimnaea HS-1 in a fed-batch process using trace elements and statistical methods. Brazilian Journal of Chemical Engineering, 27: 517-529. Go to original source...
    31. Nasri Nasrabadi M.R., Razavi S.H. (2010b): Use of response surface methodology in a fed-batch process for optimization of tricarboxylic acid cycle intermediates to achieve high levels of canthaxanthin from Dietzia natronolimnaea HS-1. Journal of Bioscience and Bioengineering, 109: 361-368. Go to original source... Go to PubMed...
    32. Park K.M., Song M.W., Lee J.H. (2008): Production of carotenoids by β-Ionone resistant mutant of Xanthophyllomyces dendrorhous using various carbon sources. Biotechnology and Bioprocess Engineering, 13: 197-203. Go to original source...
    33. Razavi S.H., Blanchard F., Marc I. (2006): UV-HPLC/ APCI MS method for separation and identification of the carotenoids produced by Sporobolomyces ruberrimus H110. Iranian Journal of Chemistry & Chemical Engineering, 25: 1-10.
    34. Reynders M.B., Rawlings D.E., Harrison S.T.L. (1997): Demonstration of the Crabtree effect in Phaffia rhodozyma during continuous and fed-batch cultivation. Biotechnology Letters, 19: 549-552. Go to original source...
    35. Vachali P., Bhosale P., Bernstein P.S. (2012): Microbial carotenoids. In: Barredo J.L. (ed.): Microbial Carotenoids from Fungi: Methods and Protocols. Springer. New York: 41-59. Go to original source...
    36. Veiga-Crespo P., Blasco L., Rosa-Dos-Santos F., Poza M., Villa T.G. (2005): Influence of culture conditions of Gordonia jacobaea MV-26 on canthaxanthin production. International Microbiology, 8: 55-58.

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