Czech J. Food Sci., 2014, 32(2):132-136 | DOI: 10.17221/291/2013-CJFS

Direct identification of bifidobacteria from probiotic supplementsOriginal Paper

Věra Buneąová1, Eva Vlková1, Vojtěch Rada1, Petra Hovorková1, ©árka Musilová1, Vladimír Kme»2
1 Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
2 Institute of Animal Physiology of Slovak Academy of Sciences, Koąice, Slovak Republic

The DNA of 14 probiotic supplements was isolated directly from various products without previous cultivation. The bifidobacteria composition declared by the manufacturer was determined by species-specific PCR. Such species found were in accordance with those listed on the products. This approach can be performed in less than 5 h and is applicable to other genera of probiotic bacteria. Bifidobacteria were also detected by culture-dependent analysis and MALDI-TOF MS. These methods drew the same results; however, they are more expensive and time consuming. So, we propose direct identification of bifidobacteria for routine quality control inspections of species composition in probiotic supplements.

Keywords: Bifidobacterium; species-specific identification

Published: April 30, 2014  Show citation

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Buneąová V, Vlková E, Rada V, Hovorková P, Musilová ©, Kme» V. Direct identification of bifidobacteria from probiotic supplements. Czech J. Food Sci. 2014;32(2):132-136. doi: 10.17221/291/2013-CJFS.
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