Czech J. Food Sci., 2013, 31(6):627-632 | DOI: 10.17221/494/2011-CJFS

Use of chemometric techniques to design a microbiological method for sulfonamide detection in milkOriginal Paper

Orlando G. Nagel1, Maria Pilar Molina2, Rafael L. Althaus1
1 Department of Basic Science, Faculty of Veterinary Sciences, National University of Litoral, Esperanza, Argentina
2 Department of Animal Science, Polytechnic University of Valencia, Valencia, Spain

We proposed an experimental design of a microbial bioassay of dichotomous response (positive or negative) using Bacillus subtilis BGA for the detection of sulfonamide residues. In the first stage, the bioassay response time was reduced to 6 h by increasing the spore concentration of B. subtilis. Then, the effects of spore, indicator, trimethoprim (TMP) concentration, and volume of the culture medium were examined with a Plackett Burman design (24-1). Finally, the effect of TMP concentration on the method detection capabilities and specificity was analysed using a logistic model with interaction. The detection capabilities of sulfonamides in milk are close to the MRLs when using 500 mg/l of TMP in the culture medium of the bioassay. It is concluded that the experimental design techniques and a logistic regression model can be used to design successfully a dichotomous response bioassay.

Keywords: B. subtilis; microbial inhibition method; bioassay; experimental design; logistic regression model

Published: December 31, 2013  Show citation

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Nagel OG, Molina MP, Althaus RL. Use of chemometric techniques to design a microbiological method for sulfonamide detection in milk. Czech J. Food Sci. 2013;31(6):627-632. doi: 10.17221/494/2011-CJFS.
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