Czech J. Food Sci., 2013, 31(3):283-291 | DOI: 10.17221/302/2012-CJFS

pH and thermal stability of anthocyanin-based optimised extracts of Romanian red onion cultivarsOriginal Paper

Simona OANCEA, Olga DRĂGHICI
Department of Agricultural Sciences, Food Industry and Environmental Protection, Lucian Blaga University of Sibiu, Sibiu, Romania

Total phenolics and anthocyanins in ten samples of Romanian red onion cultivars, extraction optimisation and pH/thermal stability were investigated. Extraction with 80% aqueous ethanol leads to an increased anthocyanin yield. The level of phenolics and anthocyanins in bulbs is highly variable and was found higher in the Red of Turda cultivar. The highest value of 99.66 mg/100 g FM was obtained for red onion dry skins. Strong acidic conditions favoured the better stability of anthocyanin extracts during 10-day storage. The first order rate constant value of anthocyanin degradation during storage calculated at different pH increased almost 17 times when pH increased from 1.0 to 9.0. Results of thermal analysis performed by differential scanning calorimetry showed that temperatures over 45°C already induced anthocyanin degradation in extracts. These results may become useful for establishing the appropriate conditions of processing and storage of anthocyanin-based foods. The obtained data on total phenolics and anthocyanins in Allium cepa L. may become relevant for future estimation studies of their daily consumption and for completing the national food composition databases.

Keywords: Allium cepa L.; phenolics; anthocyanins; pH differential; Differential Scanning Calorimetry

Published: June 30, 2013  Show citation

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OANCEA S, DRĂGHICI O. pH and thermal stability of anthocyanin-based optimised extracts of Romanian red onion cultivars. Czech J. Food Sci. 2013;31(3):283-291. doi: 10.17221/302/2012-CJFS.
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    References

    1. Cacace J.E., Mazza G. (2003): Mass transfer process during extraction of phenolic compounds from milled berries. Journal of Food Engineering, 59: 379-389. Go to original source...
    2. Corrales M., Lindauer R., Butz P., Tauscher B. (2008): Effect of heat/pressure on cyanidin-3-glucoside ethanol model solutions. Journal of Physics: Conference Series, 121: 142003. Go to original source...
    3. Dai J., Mumper R.J. (2010): Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules, 15: 7313-7352. Go to original source... Go to PubMed...
    4. Daravingas G., Cain R.F. (1968): Thermal degradation of black raspberry anthocyanin pigments in model systems. Journal of Food Science, 33: 138-142. Go to original source...
    5. Delgado-Vargas F., Paredes-López O. (2003): Natural Colourants for Food and Nutraceutical Uses. CRC Press LLC, Boca Raton. Go to original source...
    6. El-Kady S., Ammar K. (1977): Stability of Colou plum and strawberry juices during storage. Journal of Agricultural Research, 3: 99-111.
    7. Gabbott P. (ed.) (2007): Principles and applications of thermal analysis. Wiley-Blackwell, Ames: 2-5. Go to original source...
    8. Giusti M.M., R. Wrolstad E. (2001): Anthocyanins. Characterization and measurement with UV-visible spectroscopy. In: Wrolstad R.E., Schwartz S.J. (eds): Current Protocols in Food Analytical Chemistry. John Wiley & Sons, New York: F1.2.1-2.13. Go to original source...
    9. Harbone J.B., R. Grayer J. (1988): The anthocyanins. In: Harbone J.B. (ed.): The Flavonoids: Advances in Research since 1980. Chapman and Hall, London: 1-20. Go to original source...
    10. Harbourne N., Christophe J., Morgan J.D., Lyng G.J. (2008): Determination of the degradation kinetics of anthocyanins in a model juice system using isothermal and non-isothermal methods. Food Chemistry, 111: 204-208. Go to original source...
    11. Havlikova L., Mikova K. (1985): Heat stability of anthocyanins. Zeitschrift für Lebensmitteluntersuchung und -Forschung, 181: 427-432. Go to original source...
    12. Hubbermann E.M., Heins A., Stöckmann H., Schwarz K. (2006): Influence of acids, salt, sugars and hydrocolloids on the colour stability of anthocyanin rich black currant and elderberry concentrates. European Food Research and Technology, 223: 83-90. Go to original source...
    13. Jiménez N., Bohuon P., Lima J., Dornier M., Vaillant F., Pérez A.M. (2010): Kinetics of anthocyanin degradation and browning in reconstituted blackberry juice treated at high temperatures (100-180 degrees C). Journal of Agricultural and Food Chemistry, 58: 2314-2322. Go to original source... Go to PubMed...
    14. Kim S.J., Kim G.H. (2006): Quantification of quercetin in different parts of onion and its DPPH radical scavenging and antibacterial activity. Food Science and Biotechnology, 15: 39-43.
    15. Lachman J., Orsák M., Pivec V. (1999): Flavonoid antioxidants and ascorbic acid in onion (Allium cepa L.). Horticultural Science (Prague), 26: 125-134.
    16. Lachman J., Pronĕk D., Hejtmánková A., Dudjak J., Pivec V., Faitová K. (2003): Total polyphenol and main flavonoid antioxidants in different onion (Allium cepa L.) varieties. Horticultural Science (Prague), 30: 142-147. Go to original source...
    17. Lin J.-Y., Tang C.-Y. (2007): Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chemistry, 101: 140-147. Go to original source...
    18. Ly T.N., Hazama C., Shimoyamada M., Ando H., Kato K., Yamauchi R. (2005): Antioxidative compounds from the outer scales of onion. Journal of Agricultural and Food Chemistry, 53: 8183-8189. Go to original source... Go to PubMed...
    19. Marinova D., Ribarova F., Atanassova M. (2005): Total phenolics and total flavonoids in Bulgarian fruits and vegetables. Journal of the University of Chemical Technology and Metallurgy, 40: 255-260.
    20. Mazza G., Miniati E. (1993) in: Anthocyanins in Fruits, Vegetables, and Grains. CRC Press, Boca Raton: 1-28.
    21. Nuutila A.M., Puupponen-Pimiä R., Aarni M., OksmanCaldentey K.-M. (2003): Comparison of antioxidant activities of onion and garlic extracts by inhibition of lipid peroxidation and radical scavenging activity. Food Chemistry, 81: 485-493. Go to original source...
    22. Patil B.S., Pike L.M., Hamilton B.K. (1995): Changes in quercetin concentration in onion (Allium cepa L.) owing to location, growth stage and soil type. New Phytologist, 130: 349-355. Go to original source...
    23. Rivas-Gonzalo J.C. (2003): Analysis of anthocyanins. In: Santos-Buelga C., Williamson G. (eds): Methods in Polyphenol Analysis. The Royal Society of Chemistry, Cambridge.
    24. Rodrigues A.S., Pérez-Gregorio M.R., García-Falcón M.S., Simal-Gándara J., Almeida D.P.F. (2011): Effect of meteorological conditions on antioxidant flavonoids in Portuguese cultivars of white and red onions. Food Chemistry, 124: 303-308. Go to original source...
    25. Singleton V.L., Rossi J.A. Jr. (1965): Colourymetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16: 144-158. Go to original source...
    26. Slimestad R., Fossen T., Vågen I.M. (2007): Onions: a source of unique dietary flavonoids. Journal of Agricultural and Food Chemistry, 55: 10067-10080. Go to original source... Go to PubMed...
    27. Timberlake C.F., Henry B.S. (1988): Anthocyanins as natural food colorants. Progress in Clinical Biological Research, 280: 107-121.
    28. Vankar P.S., Shanker R. (2009): Dyeing of cotton, wool and silk with extract of Allium cepa. Pigment & Resin Technology, 38: 242-247. Go to original source...
    29. Wang W.-D., Xu S.-Y. (2007): Degradation kinetics of anthocyanins in blackberry juice and concentrate. Journal of Food Engineering, 82: 271-275. Go to original source...
    30. Wu X.L., Beecher G.R., Holden J.M., Haytowitz D.B., Gebhardt S.E., Piror R.L. (2006): Concentrations of anthocyanins in common foods in the United States and estimation of normal consumption. Journal of Agricultural and Food Chemistry, 54: 4069-4075. Go to original source... Go to PubMed...

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