Czech J. Food Sci., 2013, 31(2):180-188 | DOI: 10.17221/125/2012-CJFS

Differentiation of Lactobacillus species by ARDRAOriginal Paper

Kateřina Kšicová^1,†, Marta Dušková², Renáta Karpíšková^2,3

¹ Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic, 2Department of Milk Hygiene and Technology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic, 3Veterinary Research Institute, Brno, Czech Republic

The Lactobacillus species by 16S Amplified Ribosomal DNA Restriction Analysis (16S-ARDRA) was identified. Lactobacilli are bacteria often found in foodstuffs of both animal and vegetable origins. On one hand, they play an important role in the food spoilage and, on the other hand, they are used as starter cultures in food fermentation processes. The species-specific identification by traditional biochemical methods is time consuming and not always fully effective. Therefore, more efficient techniques are searched for. We focused on rapid identification of Lactobacillus isolates from different habitats. Forty-nine collection strains and isolates belonging to the genus Lactobacillus were discriminated. ARDRA was carried out with two restriction endonucleases. For the comparison of similarity, the Jaccard coefficient and clustering by the unweighted pair group method with arithmetic averages (UPGMA) were used. The percentages of similarity between profiles varied from 22% to 100% (AluI) and from 27% to 100% (MspI). This method proved applicable to the differentiation of 10 species.

Keywords: restriction analysis of the 16S rDNA gene; AluI; MspI; in silico fragmentation; species identification

Published: April 30, 2013  Show citation