Czech J. Food Sci., 2013, 31(2):156-165 | DOI: 10.17221/288/2011-CJFS

Characterisation of polyphenol oxidase from Melissa officinalis L. subsp. officinalis (lemon balm)Original Paper

Serap DOĞAN1, Yasemin AYYILDIZ1, Mehmet DOĞAN2, Ümran ALAN1, Mehmet Emin DİKEN1
1 Department of Biology
2 Department of Chemistry, Faculty of Science and Literature, University of Balikesir, Çağiº-Balikesir, Turkey

Polyphenol oxidase (PPO) from Melissa officinalis L. subsp. officinalis (lemon balm) was partially purified by ammonium sulphate precipitation and dialysis; and then it was characterised in detail in terms of pH and temperature optima, thermal stability, kinetic parameters, and inhibition properties. Based on experimental results, it was found out that (i) the optimum pH and temperature values of PPO were 6.5, 4.0, and 8.5 and 40, 50, and 60°C for catechol, 4-methylcatechol and pyrogallol substrates, respectively; (ii) the best substrate was pyrogallol due to the highest Vmax/Km value, followed by catechol and 4-methylcatechol; (iii) enzyme activity decreased due to heat denaturation of the enzyme with increasing temperature and inactivation time for all substrates; (vi) gallic acid and l-glutamic acid did not inhibit PPO; and (v) the most effective inhibitor was glutathione. Furthermore, the phenolic and protein contents of lemon balm extract were also determined according to the Folin-Ciocalteu and Bradford methods, respectively.

Keywords: lemon balm; protein; enzyme kinetics; inhibition

Published: April 30, 2013  Show citation

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DOĞAN S, AYYILDIZ Y, DOĞAN M, ALAN Ü, DİKEN ME. Characterisation of polyphenol oxidase from Melissa officinalis L. subsp. officinalis (lemon balm). Czech J. Food Sci. 2013;31(2):156-165. doi: 10.17221/288/2011-CJFS.
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    References

    1. Allahverdiyev A., Duran N., Ozguven M., Koltas S. (1994): Intiviral activity of the volatile oils of Melissa officinalis L. against Herpes simplex virus type-2. Phytomedicine, 11: 657-661. Go to original source... Go to PubMed...
    2. Alyward F., Haisman D.R. (1969): Oxidation system in fruits and vegetables - their relation to the quality of pressured products. Advances in Food Research, 17: 1-76. Go to original source... Go to PubMed...
    3. Arslan O., Temur A., Tozlu I. (1997): Polyphenol oxidase from Allium sp. Journal of Agricultural and Food Chemistry, 45: 2861-2863. Go to original source...
    4. Arslan O., Temur A., Tozlu I. (1998): Polyphenol oxidase from Malatya apricot. Journal of Agricultural and Food Chemistry, 46: 1239-1241. Go to original source...
    5. Aydemİr T. (2010): Selected kinetic properties of polyphenoloxidase extracted from Rosmarinus officinalis. International Journal of Food Properties, 13: 475-485. Go to original source...
    6. Bahtiyarca B.R., Cosge B. (2006): The essential oil of lemon balm (Melissa officinalis L.) its components and using fields. Journal of Faculty of Agricultural, 21: 116-121.
    7. Benjamin N.D., Montgomery M.W. (1973): Polyphenol oxidase of Royal Ann cherries: Purification and characterization. Journal of Food Science, 38: 799-806. Go to original source...
    8. Billaud C., Lecornu D., Nicolas J. (1996): Substrates and carboxylic acid inhibitors of partially purified polyphenoloxidase from Gum arabic. Journal of Agricultural and Food Chemistry, 44: 1668-1675. Go to original source...
    9. Bradford M. (1976): A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254. Go to original source...
    10. Dicko M.H., Hilhorst R., Gruppen H., Traore A.S., Laane C., van Berkel W.J.H., Voragen A.G.J. (2002): Comparison of content in phenolic compounds, polyphenol oxidase, and peroxidase in grains of fifty sorghum varieties from Burkina Faso. Journal of Agricultural and Food Chemistry, 50: 3780-3788. Go to original source... Go to PubMed...
    11. Dikbas N., Bagci E., Kotan R., Cakmakci R., Ozer H., Mete E., Erdogan G. (2010): Comparative antibacterial activities and chemical composition of some plants' oils against Salmonella enteritidis. Research on Crops, 11: 118-124.
    12. Djeridane A., Yousfi M., Nadjemi B., Boutassouna D., Stocker P., Vidal N. (2006): Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry, 97: 654-660. Go to original source...
    13. Dogan S. (2003): Determination of protein contents of different Origanum species collected from both different growth stages and localities. Advances in Food Sciences, 25: 150-153
    14. Dogan S., Dogan M. (2004): Determination of kinetic properties of polyphenoloxidase from Thymus (Thymus longicaulis subsp. chaubardii var. chaubardii). Food Chemistry, 88: 69-77. Go to original source...
    15. Dogan S., Salman Ü. (2007): Partial characterization of lettuce (Lactuca sativa L.) polyphenol oxidase. Europe Food Research Technology, 226: 93-103. Go to original source...
    16. Dogan S., Arslan O., Ozen F. (2005): Polyphenol oxidase activity of oregano in different stages. Food Chemistry, 91: 341-345. Go to original source...
    17. Dogan M., Arslan O., Dogan S. (2002): Substrat specifity, heat inactivation and inhibition of polyphenol oxidase from different aubergine cultivars. International Journal of Food Science and Technology, 37: 415-423. Go to original source...
    18. Dogan S., Dogan M., Arslan O. (2003a): Characterization of polyphenol oxidase from Thymus (Thymus longicaulis var. subisophyllus). Advances in Food Science, 25: 56-64.
    19. Dogan S., Dogan M., Arslan O. (2003b): Determination of some kinetic properties of polyphenol oxidase from Thymus (Thymus zygioides Griseb. var. lycaonicus (Celak) Ronninger). Advances in Food Science, 25: 130-136.
    20. Dogan S., Turan P., Dogan M., Arslan O., Alkan M. (2005a): Characterization of Ocimum basilicum L. polyphenol oxidase. Journal of Agricultural and Food Chemistry, 53: 10224-10230. Go to original source... Go to PubMed...
    21. Dogan S., Turan Y., Erturk H., Arslan O. (2005b): Characterization and purification of polyphenoloxidase from artichoke (Cynara scolymus L.). Journal of Agricultural and Food Chemistry, 53: 776-785. Go to original source... Go to PubMed...
    22. Dogan S., Turan P., Dogan M. (2006): Some kinetic properties of polyphenol oxidase from Thymbra spicata L. var. spicata. Process Biochemistry, 41: 2379-2385. Go to original source...
    23. Dogan S., Turan P., Dogan M., Alkan M., Arslan O. (2007): Inhibition kinetics of polyphenoloxidase by glutamic acid. Europe Food Research Technology, 225: 67-73. Go to original source...
    24. Dogan S., Dogan M., Arslan O. (2009): Prevention of Enzymatic Browning and its Inhibition. Food Processing: Methods, Techniques and Trends. Novapublishers, New York.
    25. Durigan F., Mattiuz B.H., James T. (2006): Cultivar affects browning susceptibilty of fresh cut star fruit slices. Science Agriculture, 63: 1-4. Go to original source...
    26. Erat M., Sakiroglu H., Kufrevioglu I.O. (2006): Purification and characterization of polyphenol oxidase from Ferula sp. Food Chemistry, 95: 503-508. Go to original source...
    27. Guanata Y.Z., Sapis J.C., Moutonet M. (1987): Substrates and aromatic carboxlic and inhibitors of grape polyphenoloxidases. Phytochemistry, 26: 1573-1575. Go to original source...
    28. Gundogmaz G., Dogan S., Arslan O. (2003): Some kinetic properties of polyphenol oxidase obtained from various Salvia species (Salvia viridis L., Salvia virgata Jacq. and Salvia tomentosa Miller). Food Science and Technology International, 9: 309-315. Go to original source...
    29. Javovitz-Klapp A., Richard F., Nicolas J. (1989): Polyphenoloxidase from apple, partial purification and some properties. Phytochemistry., 28: 2903-2907. Go to original source...
    30. Kahn V., Andrawis A. (1985): Inhibition of mushroom tyrosinase by tropolone. Phytochemistry, 24: 905-908. Go to original source...
    31. Kaur C., Kapoor H.C. (2002): Antioxidant activity and total phenolic content of some Asian vegetables. International Journal of Food Science and Technology, 37: 153-161. Go to original source...
    32. Lee C.Y., Smith N.L., Pennesi A.P. (1983): Polyphenol oxidase from DeChaunac grapes. Journal of the Science of Food and Agricultural, 34: 987-991. Go to original source...
    33. Lee C.Y., Withaker J.R. (1995): Enzymatic Browning and its Prevention. American Chemical Society, Washington.
    34. Macrae A.R., Duggleby R.G. (1968): Substrates and inhibitors of potato tuber phenolose. Phytochemistry, 7: 855-861. Go to original source...
    35. Mayer A.M., Harel E. (1979): Polyphenol oxidase in plants. Phytochemistry, 18: 193-215. Go to original source...
    36. Mazzafera P., Robinson S.P. (2000): Characterization of polyphenol oxidase in coffee. Phytochemistry, 55: 285-296. Go to original source... Go to PubMed...
    37. Moradkhani H., Sargsyan E., Bibak H., Naseri B., Sadat-Hosseini M., Fayazi-Barjin A., Meftahizade H. (2010): Melissa officinalis L., a valuable medicine plant. A review. Journal of Medicinal Plants Research, 4: 2753-2759.
    38. Oktay M., Kufrevioglu I., Kocacaliskan I., Sakiroglu H. (1995): Polyphenoloxidase from Amasya apple. Journal of Food Science, 60: 495-499. Go to original source...
    39. Park E.Y., Luh B.S. (1985): Polyphenol oxidase of kiwi fruit. Journal of Food Science, 50: 678-684. Go to original source...
    40. Paul B., Gowda L.R. (2000): Purification and characterization of polyphenol oxidase from the seeds of field bean (Dalichos lablab). Journal of Agricultural and Food Chemistry, 48: 3839-3846. Go to original source... Go to PubMed...
    41. Rivas N.J., Withaker J.R. (1973): Purification and some properties of two polyphenol oxidases from Bartlett pears. Plant Physiology, 52: 501-507. Go to original source... Go to PubMed...
    42. Robert C., Rouch C., Cadet F. (1997): Inhibition of palmito (Acanthoenix rubra) polyphenoloxidase by carboxylic acids. Food Chemistry, 59: 355-360. Go to original source...
    43. Sanchez-Ferrer A., Laveda F., Garcia-Carmano F. (1993): Cresolase activity of potato tuber partially purified in a two-phase partition system. Journal of Agricultural and Food Chemistry, 41: 1225-1228. Go to original source...
    44. Singleton V.L., Rossi J.A. (1965): Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology, 16: 144-145. Go to original source...
    45. Unal M.U. (2007): Properties of polyphenol oxidase from Anamur banana (Musa cavendishii). Food Chemistry, 100: 909-913. Go to original source...
    46. Voet D., Voet J.G. (2003): Biochemistry. Wiley, USA.
    47. Wesche-Ebeling P., Montgomery M.W. (1990): Strawberry polyphenoloxidase: Extraction and partial characterization. Journal of Food Science, 55: 1320-1325. Go to original source...
    48. Ziyan E., Pekyardimci ª. (2003): Characterization of polyphenol oxidase from Jerusalem artichoke (Helianthus tuberosus). Turkish Journal of Chemistry, 27: 217-225.

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