Czech J. Food Sci., 2012, 30(4):364-368 | DOI: 10.17221/108/2011-CJFS

Aqueous extraction of limonin from Citrus reticulate Blanco

Can Liu1, Jing Liu1, Yonghai Rong1, Nvyong Liang2, Long Rong1
1 Key Laboratory of the Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, P.R.China
2 Jiangmen Haolun Corporation, Jiangmen, P.R. China

The replacement of organic solutions in the extraction of limonin from citrus seeds with an alkaline solution was investigated. This method was based on the reversible conversion of limonin to limonoate A-ring lactone via ring-opening of D-ring lactone at different pH values. The extraction conditions, optimised using Taguchi experimental design, were as follows: pH 11, temperature 70°C, alkaline solution/seeds ratio 20:1 (v/w), ultrasonic power 800 W for 30 minutes. A yield of 7.5 mg/g (limonin/citrus seeds) of 98% pure limonin was obtained.

Keywords: optimisation; citrus seeds; conversion; Taguchi approach; ultrasonic extraction

Published: August 31, 2012  Show citation

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Liu C, Liu J, Rong Y, Liang N, Rong L. Aqueous extraction of limonin from Citrus reticulate Blanco. Czech J. Food Sci. 2012;30(4):364-368. doi: 10.17221/108/2011-CJFS.
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