Czech J. Food Sci., 2012, 30(1):83-88 | DOI: 10.17221/7/2011-CJFS

Outdoor environment as a source of Listeria monocytogenes in food chain

Tereza Gelbíčová1,3, Renáta Karpíšková1,2
1 National Institute of Public Health Prague, Brno, Czech Republic
2 University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic
3 Faculty of Science, Masaryk University Brno, Brno, Czech Republic

We monitored the presence of Listeria monocytogenes in environmental sources and to evaluate the phenotypic and molecular characteristics of the isolates recovered. L. monocytogenes was isolated in 12 (11.2%) of the 107 samples from the wild, farm environment, and vegetation. Most isolates (83.3%) were of serotype 1/2a and the remainder (2) were of serotype 4b. All 12 isolates were susceptible to the whole range of antimicrobials tested. These12 strains were carriers of the virulence genes prfA, hlyA, actA, plcA, plcB, inlA, inlB, inlC, and inlJ. The detection of the inlA gene in 4 (33.3%) of 12 strains using the PCR-RFLP suggests the potential of some of these strains to penetrate into epithelial cells of the intestinal barrier. Macrorestriction analysis also confirmed clonal identity of some environmental isolates with food and human isolates. These results indicate that the external environment is a source of potentially pathogenic strains of L. monocytogenes.

Keywords: virulence; listeria; antimicrobial resistance; restriction fragment length polymorphism; pulsed-field gel electrophoresis (PFGE)

Published: February 29, 2012  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Gelbíčová T, Karpíšková R. Outdoor environment as a source of Listeria monocytogenes in food chain. Czech J. Food Sci. 2012;30(1):83-88. doi: 10.17221/7/2011-CJFS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Aurora R., Prakash A., Prakash S., Rawool D.B., Barbuddhe S.B. (2008): Comparison of PI-PLC based assays and PCR along with in vivo pathogenicity tests for rapid detection of pathogenic Listeria monocytogenes. Food Control, 19: 641-647. Go to original source...
    2. Bierne H., Sabet C., Personnic N., Cossart P. (2007): Internalins: a complex family of leucine-rich repeat-containing proteins in Listeria monocytogenes. Microbes and Infection, 9: 1156-1166. Go to original source... Go to PubMed...
    3. Chen J., Luo X., Jiang L., Jin P., Wei W., Liu D., Fang W. (2009a): Molecular characteristics and virulence potential of Listeria monocytogenes isolates from Chinese food systems. Food Microbiology, 26: 103-111. Go to original source... Go to PubMed...
    4. Chen J., Jiang L., Chen X., Lou X., Chen Y., Yu Y., Tian G., Liu D., Fang W. (2009b): Listeria monocytogenes serovar 4a is a possible evolutionary intermediate between L. monocytogenes serovars 1/2a and 4b and L. innocua. Journal of Microbiology and Biotechnology, 19: 238-249. Go to PubMed...
    5. Colburn K.G., Kaysner CH.A., Carlos Abeyta J.R., Wekell M.M. (1990): Listeria species in a California coast estuarine environment. Applied and Environmental Microbiology, 56: 2007-2011. Go to original source... Go to PubMed...
    6. CLSI (2006); Performance standards for antimicrobial susceptibilty testing; sixteenth informational supplement. NCCLS document M100-S16. Clinical and Laboratory Standards Institute, Wayne: 44-55.
    7. Comité de l'antibiogramme de la Société Française de Microbiologie (2005): Concentrations et diamètres critiques pour les diverses classes d'antibiotiques. Société Française de Microbiologie:11-12.
    8. D'Agostino M., Wagner M., Vazquez-Boland J.A., Kuchta T., Karpiskova R., Hoorfar J., Novella S., Scortti M., Ellison J., Murray A., Fernandes I., Kuhn M., Pazlarova J., Heuvelink A., Cook N.A. (2004): A validated PCR-based method to detect Listeria monocytogenes using raw milk as a food model-towards an international standard. Journal of Food Protection, 67: 1646-1655. Go to original source... Go to PubMed...
    9. Doumith M., Cazalet Ch., Simoes N., Frangeul L., Jacquet Ch., Kunst F., Martin P., Cossart C., Glaser P., Buchrieser C. (2004a): New aspects regarding evolution and virulence of Listeria monocytogenes revealed by comparative genomics and DNA arrays. Infection and Immunity, 72: 1072-1083. Go to original source... Go to PubMed...
    10. Doumith M., Buchrieser C., Glaser P., Jacquet C., Martin P. (2004b): Differentiation of the major Listeria monocytogenes serovars by multiplex PCR. Journal of Clinical Microbiology, 42: 3819-3822. Go to original source... Go to PubMed...
    11. Esteban J.I., Oporto B., Aduriz G., Juste R.A., Hurtado A. (2009): Faecal shedding and strain diversity of Listeria monocytogenes in healthy ruminants and swine in Northern Spain. BMC Veterinary Research, 5: 2. Go to original source... Go to PubMed...
    12. EN ISO 11290-1:1996/Amd.1:2004: Microbiology of food and animal feeding stuffs-Horizonatl method for the detection and enumeration of Listeria monocytogenes - Part 1: Detection Method.
    13. Farber J.M., Peterkin P.I. (1991): Listeria monocytogenes, a food-borne pathogen. Microbiological Reviews, 55: 476-511. Go to original source... Go to PubMed...
    14. Freitag N.E., Port G.C., Miner M.D. (2009): Listeria monocytogenes - from saprophyte to intracellular pathogen. Nature Reviews Microbiology, 7: 623-628. Go to original source... Go to PubMed...
    15. Fugett E.B., Schoonmaker-Bopp D., Dumas N.B., Corby J., Wiedman M. (2007): Pulsed-field gel electrophoresis (PFGE) analysis of temporally matched Listeria monocytogenes isolates from human clinical cases, foods, ruminants farms, and urban and natural environments reveals source-associated as well as widely distributed PFGE types. Journal of Clinical Microbiology, 45: 865-873. Go to original source... Go to PubMed...
    16. Gelbíčová T., Šťástková Z., Pospíšilová M., Karpíšková R. (2008): Charakteristika izolátů Listeria monocytogenes z mléčných výrobků. Veterinářství, 58: 324-326.
    17. Gray M.J., Freitag N.E., Boor K.J. (2006): How the bacterial pathogen Listeria monocytogenes mediates the switch from environmental Dr. Jekyll to pathogenic Mr. Hyde. Infection and Immunity, 74: 2505-2512. Go to original source... Go to PubMed...
    18. Ivanek R., Gröhn Y.T., Wiedmann M. (2006): Listeria monocytogenes in multiple habitats and host populations: review of available data for mathematical modeling. Foodborne Pathogens and Disease, 3: 319-336. Go to original source... Go to PubMed...
    19. Jaradat Z.W., Schutze G.E., Bhunia A.K. (2002): Genetic homogeneity among Listeria monocytogenes strains from infected patients and meat products from two geographic locations determined by phenotyping, ribotyping and PCR analysis of virulence genes. International Journal of Food Microbiology, 76: 1-10. Go to original source... Go to PubMed...
    20. Kalorey D.R., Kurkure N.V., Warke S.R., Rawool D.B., Malík S.V.S., Barbuddhe S.B. (2006): Isolation of pathogenic Listeria monocytogenes in faeces of wild animals in captivity. Comparative Immunology, Microbiology and Infectious Diseases, 29: 295-300. Go to original source... Go to PubMed...
    21. Karpíšková R., Gelbíčová T. (2008): Charakteristika a prevalence klonů Listeria monocytogenes izolovaných od pacientů v letech 2001-2008 v České republice. Epidemiologie, mikrobiologie, imunologie, 57: 137-140.
    22. Karpíšková R., Gelbíčová T. (2009): The role of typing methods in epidemiological investigations of listeriosis. In: 3 rd Congress of European Microbiologists FEMS. Sweden: 232.
    23. Kaur S., Malík S.V.S., Bhilegaonkar K.N., Vaidya V.M., Barbuddhe S.B. (2010): Use of phospholipase-C assay, in vivo pathogenicity assays and PCR in assessing the virulence of Listeria spp. Veterinary Journal, 184: 366-370. Go to original source... Go to PubMed...
    24. Liu D., Lawrence M.L., Austin F.W., Ainsworth A.J. (2007): A multiplex PCR for species - and virulence - specific determination of Listeria monocytogenes. Journal of Microbiological Methods, 71: 133-140. Go to original source... Go to PubMed...
    25. Lyautey E., Lapen D.R., Wilkes G., McCleary K., Pagotto F., Tyler K., Hartmann A., Piveteau P., Rieu A., Robertson W.J., Medeiros D.T., Edge T.A., Gannon V., Topp E. (2007): Distribution and characteristics of Listeria monocytogenes isolates from surface waters of the south nation river watershed, Ontario, Canada. Applied and Environmental Microbiology, 73: 5401-5410. Go to original source... Go to PubMed...
    26. MacGowan A.P., Bowker K., McLauchlin J., Bennett P.M., Reeves D.S. (1994): The occurrence and seasonal changes in the isolation of Listeria spp. in shop bought food stuffs, human faeces, sewage and soil from urban sources. International Journal of Food Microbiology, 21: 325-334. Go to original source... Go to PubMed...
    27. Mammina C., Aleo A., Romani C., Pellissier N., Nicoletti P., Pecile P., Nastasi A., Pontello M.M. (2009): Characterization of Listeria monocytogenes isolates from human listeriosis cases in Italy. Journal of Clinical Microbiology, 47: 2925-2930. Go to original source... Go to PubMed...
    28. Pulse-net Europe (2002): Standardized protocol for molecular subtyping of Listeria monocytogenes by pulsed-field gel electrophoresis (PFGE): 10. Available at http://www.pulsenet-europe.org
    29. Rodas-Suárez O.R., Flores-Pedroche J.F., Betancourt-Rule J.M., Quiñones-Ramírez E.I., VázquezSalinas C. (2006): Occurrence and antibiotic sensitivity of Listeria monocytogenes strains isolated from oysters, fish, and estuarine water. Applied and Environmental Microbiology, 72: 7410-7412. Go to original source... Go to PubMed...
    30. Rousseaux S., Olier M., Lemaître J.P., Piveteau P., Guzzo J. (2004): Use of PCR-restriction fragment length polymorphism of inlA for rapid screening of Listeria monocytogenes strains deficient in the ability to invade Caco-2 cells. Applied and Environmental Microbiology, 70: 2180-2185. Go to original source... Go to PubMed...
    31. Vázquez-Boland J.A., Kuhn M., Berche P., Chakraborty T., Domínguez-Bernal G., Goebel W., GonzálezZorn B., Wehland J., Kreft J. (2001): Listeria pathogenesis and molecular virulence determinants. Clinical Microbiological Reviews, 14: 584-640. Go to original source... Go to PubMed...
    32. Weis J., Seeliger H.P.R. (1975): Incidence of Listeria monocytogenes in nature. Applied Microbiology, 30: 29-32. Go to original source... Go to PubMed...
    33. Welshimer H.J., Donket-Voet J. (1971): Listeria monocytogenes in nature. Applied Microbiology, 21: 516-519. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content