Czech J. Food Sci., 2011, 29(10):S93-S101 | DOI: 10.17221/307/2011-CJFS

Utilization of DNA microarrays for detection and identification of selected Fusarium species from the Czech Republic

Lucie Pavlátová1, David Novotný2, Jan Hodek1, Jana Chrpová3, Jaroslava Ovesná1
1 Department of Molecular Biology,
2 Department of Mycology and

Fusarium is a serious phytopathogenic fungal genus with producting of many kinds of highly toxic secondary metabolites - mycotoxins. The consumption of Fusarium contaminated food and feed can cause dangerous mycotoxicoses both in humans and animals, therefore the detection of a wider range of Fusarium species in the samples of crops is very important. The aim of our work was to test the reliability of detection and identification of three Fusarium species in infected wheat grains by DNA microarrays versus classical mycological methods and by specific PCR. The in-house DNA microarrays for the detection and identification of the selected Fusarium species by using oligonucleotides probes were prepared. For hybridisation on DNA microarrays fluorescent labelled PCR products were used of part of the translation elongation factor 1 alpha. The conditions of hybridisation were optimised on fungal template DNA. The method of DNA microarrays was verified on artificially infected samples of wheat and tested on unknown infected wheat samples with simultaneous analysis by classical mycological methods and by specific PCR.

Keywords: DNA microarrays; fluorescent labelling; Fusarium; oligonucleotide probe; PCR

Published: December 31, 2011  Show citation

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Pavlátová L, Novotný D, Hodek J, Chrpová J, Ovesná J. Utilization of DNA microarrays for detection and identification of selected Fusarium species from the Czech Republic. Czech J. Food Sci. 2011;29(Special Issue):S93-101. doi: 10.17221/307/2011-CJFS.
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