Czech J. Food Sci., 2011, 29(1):31-34 | DOI: 10.17221/151/2010-CJFS

Development of a scientific study for accessing the criteria under Commission Regulation (EC) 2073/2005 on traditional Slovak sheep cheese "bryndza"

Lenka Cabanová1, Oµga ©kuntová1, Daniela Matisová1, Monika Pipová2
1 State veterinary and Food Institute, Dolny Kubin, Slovakia
2 Department of Food Hygiene and Food Technology, University of Veterinary Medicine, Koąice, Slovak Republic

A scientific shelf-life study for Listeria monocytogenes in the typical Slovak cheese "bryndza" was performed in accordance with the requirements of the Commission Regulation (EC) 2073/2005. Based on the previous positive findings of L. monocytogenes in the final products, the producer decided to perform laboratory tests, the results of which would allow him a different evaluation of these positive results. Both the physico-chemical (pH, aw) and microbiological examinations of "bryndza" cheese stored at 5.8-6.2°C were performed every two days till the end of the product shelf-life (7 days). Microbiological analyses were performed after artificial contamination of the final product with a mixture of three L. monocytogenes strains. The growth potential of L. monocytogenes was calculated as the difference in the counts of this bacterium between the last day and the first day of the test. The Slovak traditional "bryndza" cheese has been found not to support the growth of L. monocytogenes. Thus, the counts of L. monocytogenes must not exceed 50 CFU/g at the beginning and 20 CFU/g at the end of the product shelf-life in order to ensure its safety for the consumer.

Keywords: Listeria monocytogenes; traditional cheese; Slovakia

Published: February 28, 2011  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Cabanová L, ©kuntová O, Matisová D, Pipová M. Development of a scientific study for accessing the criteria under Commission Regulation (EC) 2073/2005 on traditional Slovak sheep cheese "bryndza". Czech J. Food Sci. 2011;29(1):31-34. doi: 10.17221/151/2010-CJFS.
Download citation

References

  1. Aygun O., Pehlivanlar S. (2006): Listeria spp. in the raw milk and dairy products in Antakya, Turkey. Food Control, 17: 676-679. Go to original source...
  2. Blaľková M., Karamonová L., Fukal L., Rauch P. (2005): Listeria monocytogenes - nebezpečný patogen a jeho detekce v potravinách. Chemické listy, 99: 467-473.
  3. Boyer R.R., Matak K., Sumner S.S., Meadows B., Williams R.C., Eifert J.D., Birbari W. (2009): Survival of Listeria monocytogenes, Listeria innocua and Lactic acid bacteria in chill brines. Journal of Food Science, 74: 219-223. Go to original source... Go to PubMed...
  4. Buazzi M.M., Johnson M.E., Marth E.H.(1992): Survival of Listeria monocytogenes during the manufacture and ripening of Swiss cheese. Journal of Dairy Science, 75: 380-386. Go to original source... Go to PubMed...
  5. Casadeo M.A., Esteves de Matos R., Harrison S.T., Gaye J.E. (1998): Heat resistance of Listeria monocytogenes in dairy products as affected by the growth medium. Journal of Applied Microbiology, 84: 234-239. Go to original source... Go to PubMed...
  6. Cataldo G., Conte M.P., Chiarini F., Seganti L., Ammendolia M.G., Superti F., Longhi C. (2007): Acid adaptation and survival of Listeria monocytogenes in Italian-style soft cheese. Journal of Applied Microbiology, 103: 185-193. Go to original source... Go to PubMed...
  7. Cottin J., Picard-Bonnaud F., Carbonelle B. (1990): Study of Listeria monocytogenes survival during the preparation and the conservation of two kinds of dairy product. Acta Microbiologica Hungarica, 37: 119-122. Go to PubMed...
  8. Doyle E., Mayyotta A.S., Wang T., Wiseman D.W., Scott V.N. (2001): Heat resistance of Listeria monocytogenes. Journal of Food Protection, 64: 410-429. Go to original source... Go to PubMed...
  9. El-Gazzar F.E., Marth E.H. (1991): Survival of Listeria monocytogenes in a food colorant derived from red beets. Journal of Dairy Science, 74: 81-86. Go to original source... Go to PubMed...
  10. Gahan C.G., O´Driscoll B., Hill C. (1996): Acid adaptation of Listeria monocytogenes can enhance survival in acidic foods and during milk fermentation. Applied Environmental Microbiology, 62: 3128-3132. Go to original source... Go to PubMed...
  11. Guidance document on Listeria monocytogenes shelf-life studies for ready-to-eat foods, under Regulation (EC) No. 2073/2005 of 15 November 2005 on microbiological criteria for foodstuff (2008). Available at http://ec.europa.eu/food/food/biosafety/salmonella/docs/guidoc_listeria_monocytogenes_en.pdf
  12. Hitchs S.J., Lund B.M. (2008): The survival of Listeria monocytogenes in cottage cheese. Journal of Applied Microbiology, 70: 308-341. Go to original source... Go to PubMed...
  13. Hokl J., ©těpánek M. (1962): Hygiena potravin II. Mléko a mléčné výrobky. SZN, Praha.
  14. Lundén J., Tolvanen R., Korkeala H. (2004): Human listeriosis outbreaks linked to dairy products in Europe. Journal of Dairy Science, 87: 6-12. Go to original source...
  15. Mataragas M., Stergiou V., Nychas G.J.E. (2008): Modelling survival of Listeria monocytogenes in the traditional greek soft cheese Katiki. Journal of Food Protection, 71: 1835-1845. Go to original source... Go to PubMed...
  16. Oyarzábal O.A., Nogueira M.C.L., Gombas D.E. (2003): Survival of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella in juice concentrates. Journal of Food Protection, 66: 1595-1598. Go to original source... Go to PubMed...
  17. Phan-Thanh L., Mahouin F., Aligé S. (2000): Acid responses of Listeria monocytogenes. International Journal of Food Microbiology, 55: 21-126. Go to original source... Go to PubMed...
  18. Rogga K.J., Samelis J., Kakouri A., Katatsiari M.C., Savvaidis I.N., Kontominas M.G. (2005): Survival of Listeria monocytogenes in Galotyri, a traditional Greek soft acid-curd cheese, stored aerobically at 4°C and 12°C. International Dairy Journal, 15: 59-67. Go to original source...

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.