Czech J. Food Sci., 2010, 28(6):516-519 | DOI: 10.17221/1838-CJFS
Immunohistochemical detection of wheat protein in model samples
- 1 Department of Vegetable Food and Agriculture Production, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic
- 2 Veterinary Research Institute, Brno, Czech Republic
The study focused on the optimisation of immunohistochemical examination for gluten content detection in model samples (pork meat with wheat semi-smooth flour, pork meat with wheat protein edible vital). The best results were achieved with immunohistochemical method based on ABC (avidin-biotin complex) method utilising polyclonal antibodies diluted 1:1000. The results demonstrate that for pure wheat protein detection, the utilisation of immunohistochemical detection, which can detect as little as 0.1% of the added wheat protein, is more advantageous, while the commonly used ELISA method reliably proves this additive approximately from 0.4% upwards.
Keywords: food allergens; gluten; celiac disease; identification
Published: December 31, 2010 Show citation
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