Czech J. Food Sci., 2010, 28(5):392-406 | DOI: 10.17221/217/2009-CJFS

Effect of milk chocolate supplementation with lyophilised Lactobacillus cells on its attributes

Dorota Żyżelewicz1, Ewa Nebesny1, Ilona Motyl2, Zdzisława Libudzisz2
1 Chair of Starch Processing and Confectionery, Institute of Chemical Food Technology and
2 Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Technical University of Lodz, Lodz, Poland

Manufacturing of novel foodstuffs supplemented with live probiotic bacteria has recently been intensively investigated. The supplementation of confectionery with probiotics is troublesome since some unit technological processes are conducted at high temperatures and the products are usually stored at ambient temperature. Our group has developed a method of the production of milk chocolate, sweetened with either sucrose or isomalt and aspartame, containing 32, 36, or 40 g/100 g fat, and supplemented with live cells of probiotic bacterial strains: Lactobacillus casei and paracasei. This new milk chocolate displayed the same sensory properties as the reference, probiotic-free chocolate. The number of live bacterial cells was maintained at the functional level of 106 / 108 cfu/g after keeping for 12 months irrespective of the temperature. The highest number of live probiotic bacteria survived in the chocolate kept at 4°C. Thus the product can be regarded as functional food.

Keywords: milk chocolate; properties of chocolate; lyophilisate; LAB

Published: October 31, 2010  Show citation

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Żyżelewicz D, Nebesny E, Motyl I, Libudzisz Z. Effect of milk chocolate supplementation with lyophilised Lactobacillus cells on its attributes. Czech J. Food Sci. 2010;28(5):392-406. doi: 10.17221/217/2009-CJFS.
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References

  1. Alamprese C., Foschino R., Rossi M., Pompei C., Savani L. (2002): Survival of Lactobacillus johnsonii La1 and influence of its addition in retail-manufactured ice cream produced with different sugar and fat concentrations. International Dairy Journal, 12: 201-208. Go to original source...
  2. Aragon-Alegro L.C., Alegro J.H.A., Cardarelli H.R., Chiu M.C., Saad S.M.I. (2007): Potentially probiotic and symbiotic chocolate mousse. LWT-Food Science and Technology, 40: 669-675. Go to original source...
  3. Blanchette L., Roy D., Bélanger G., Gauthier S.F. (1996): Production of cottage cheese using dressing fermented by bifidobacteria. Journal of Dairy Science, 79: 8-15. Go to original source...
  4. Chevalley J. (1991): An adaptation of the Casson equation for the rheology of chocolate. Journal of Texture Studies, 22: 219-229. Go to original source...
  5. Cukrowska B., Ceregra A., Rosiak I., Libudzisz Z., Motyl I., Klewicka E. (2007): Effect of oral dosage of probiotic bacteria Lactobacillus on composition of gut ecosystem, immune system and course of the food allergy in infants. Report of Grant KBN PB 777/ P05/2004/26. (In Polish)
  6. Daigle A., Roy D., Bélanger G., Vuillemard J.C. (1999): Production of probiotic cheese (Cheddar-like cheese) using enriched cream fermented by Bifidobacterium infantis. Journal of Dairy Science, 82: 1081-1091. Go to original source... Go to PubMed...
  7. Dinakar P., Mistry V.V. (1994): Growth and viability of Bifidobacterium bifidum in Cheddar cheese. Journal of Dairy Science, 77: 2854-2864. Go to original source... Go to PubMed...
  8. Dokuritsu Gyosei Hojni Suisan Sogo Kenky & Yakult Honsha (2004): Preparing feed for fish and shellfish, comprises mixing seaweed with a carbohydrate type solution enzyme and fermenting using Lactobacillus e.g. Lactobacillus plantarum, Lactobacillus casei or Lactobacillus rhamnosus. Japanese Patent JP2004298080.
  9. Finke A. (1965): Handbuch der Kakaoerzeugnisse. Springer, Berlin, Heidelberg, New York: 357. Go to original source...
  10. Gobbetti M., Corsetti A., Smacchi E., Zocchetti A., De Angelis M. (1998): Production of Cvescerza Cheese by incorporation of bifidobacteria. Journal of Dairy Science, 81: 37-47. Go to original source...
  11. Gomes Da Cruz A., Alonso Buriti F.C., Batista De Souza C.H., Fonseca Faria J.A., Isay Saad S.M. (2009): Probiotic cheese: health benefits, technological and stability aspects. Trends in Food Science & Technology, 20: 344-354. Go to original source...
  12. Heenan C.N., Adams M.C., Hosken R.W., Fleet G.H. (2004): Survival and sensory acceptability of probiotic microorganisms in a nonfermented frozen vegetarian dessert. LWT-Food Science and Technology, 37: 461-466. Go to original source...
  13. Leblanc J.G., Garro M.S., Savoy De Giori G., Font De Valdez G. (2004): A novel functional soy-based food fermented by lactic acid bacteria: effect of heat treatment. Journal of Food Science, 69: M246-M250. Go to original source...
  14. Matilla-Sandholm T., Myllärinen P., Crittenden R., Mogensen G., Fondén R., Saarela M. (2002): Technological challenges for future probiotic foods. International Dairy Journal, 12: 173-182. Go to original source...
  15. Mazigh D. (1994): Microbiology of chocolate. In: Beckett S.T. (ed.): Industrial Chocolate Manufacture and Use. 2nd Ed. Chapman and Hall, New York: 314. Go to original source...
  16. McFarlane I. (1994): Instrumentation. In: Beckett S.T. (ed.): Industrial Chocolate Manufacture and Use. 2nd Ed. Chapman and Hall, New York: 305. Go to original source...
  17. Modzelewska-Kapitula M., Klebukowska L., Kornacki K. (2007): Influence of inulin and potentially probiotic Lactobacillus plantarum strain on microbiological quality and sensory properties of soft cheese. Polish Journal of Food & Nutrition Sciences, 57: 143-146.
  18. Nebesny E., Żyżelewicz D. (2005): Effect of lecithin concentration on properties of sucrose-free chocolate masses sweetened with isomalt. European Food Research and Technology, 220: 131-135. Go to original source...
  19. Nebesny E., Żyżelewicz D. (2006): Properties of chocolates enriched with viable lactic acid bacteria. Deutsche Lebensmittel-Rundschau, 102: 27-32.
  20. Nebesny E., Pierzgalski T., Żyżelewicz D. (1998). The effect of polyglycerol polyricinoleate on the rheologic properties of dark chocolate masses. Food, Technology, Quality, 17: 39-43.
  21. Nebesny E., Żyżelewicz D., Pierzgalski T. (2002): Effect of the time of action of emulsifiers on rheological properties of natural chocolate mass. Zeszyty Naukowe Politechniki Łódzkiej "Chemia Spożywcza i Biotechnologia", 893: 91-101.
  22. Nebesny E., Żyżelewicz D., Motyl I., Libudzisz Z. (2005): Properties of sucrose-free chocolates enriched with viable lactic acid bacteria. European Food Research and Technology, 220: 358-362. Go to original source...
  23. Ong L., Shah N.P. (2009): Probiotic Cheddar cheese: influence of ripening temperatures on survival of probiotic microorganisms, cheese composition and organic acid profiles. LWT- Food Science and Technology, 42: 1260-1268. Go to original source...
  24. Ranganathan N. (2004): New compositions comprising least one probiotic bacteria which reduces creatinine and blood urea nitrogen levels, useful for augmenting kidney function, treating renal failure, or maintaining gastrointestinal health. United States Patent US20040197352.
  25. Report of a Joint Fao/who Expert Consultation (2001): Health and nutritional properties of probiotics in food including milk with live lactic acid bacteria. Cordoba, Argentina.
  26. Report a Joint FAO/WHO Working Group (2002): Guidelines for the Evaluation of Probiotics in Food. London, Ontario.
  27. Ryukyu Bioresource Kaihatsu K.K. (2004): Fermented substance having ACE inhibitory effect useful as foodstuff raw material in health foods and drinks, is obtained by fermenting mugwort. Japanese Patent JP2004267100.
  28. Saarela M., Virkajärvi I., Nohynek L., Vaari A., Mättö J. (2006): Fibres as carries for Lactobacillus rhamnosus during freeze-drying and storage in apple juice and chocolate-coated breakfast cereals. International Journal of Food Microbiology, 112: 171-178. Go to original source... Go to PubMed...
  29. Sakamoto Karyo K.K. (2004): Vegetable juice drink contains specified amount of fermented vegetable juice, which is obtained by fermenting vegetable juice with Lactobacillus e.g. Lactobacillus helveticus. Japanese Patent JP2004254528.
  30. Salem M.M.E., Fathi F.A., Awad R.A. (2005). Production of probiotic ice cream. Polish Journal of Food and Nutrition Sciences, 14/55: 267-271.
  31. Schmitt H. (1994): Fractionated and modified lecithin products - new application possibilities. Food Marketing & Technology, 8: 21-22, 24-25.
  32. Sokmen A., Gunes G. (2006): Influence of some bulk sweeteners on rheological properties of chocolate. LWT -Food Science and Technology, 39: 1053-1058. Go to original source...
  33. Stanton C., Ross R.P., Fitzgerald G.F., Van Sinderen D. (2005): Fermented functional foods based on probiotics and their biogenic metabolites. Current Opinion in Biotechnology, 16: 198-203. Go to original source... Go to PubMed...
  34. Steiner E.H. (1958): A new rheological relationship to express the flow properties of melted chocolate. International Chocolade Revue, 13: 290.
  35. Sugiyama M., Nomura K., Oku H., Dogaito H. (2006): Fermented drink is obtained by adding sake lees, distilled spirit residue or its extract to fermentation raw material containing milk, fruit juice and/or vegetable juice, and fermenting using plant lactic acid bacteria. Japanese Patent JP2006042796.
  36. Woods L.C. (1976): Emulsifiers in the confectionery industrie. Gordian, 76(2): 53-57.
  37. Yoon K.Y., Woodams E.E., Hang Y.D. (2006). Production of probiotic cabbage juice by lactic acid bacteria. Bioresource Technology, 97: 1427-1430. Go to original source... Go to PubMed...

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