Czech J. Food Sci., 2010, 28(4):326-332 | DOI: 10.17221/153/2010-CJFS

Resistance of Listeria monocytogenes biofilms to disinfectants 326

Sabina PURKRTOVÁ, Hana TUROŇOVÁ, Tereza PILCHOVÁ, Kateřina DEMNEROVÁ, Jarmila PAZLAROVÁ
Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic

We studied the optimal conditions for the biofilm development by Listeria monocytogenes on a model system represented by microtiter plates, and also for determined some effective disinfectant agents. Listeria monocytogenes ATCC 13932 and an industrial isolate of Listeria monocytogenes Lm-24 were compared as to their abilities to form biofilms. The starting concentration of the cells leading to the most reproducible results was 0.5 McFarland. The temperatures tested ranged between 8°C to 37°C, the optimal values to form biofilm in buffered peptone water (BPW) with 0.05% glucose were 25°C and 30°C. Under comparable conditions the persistent strain L. monocytogenes Lm-24 constituted more massive biofilm than did the reference strain. The following disinfectants were applied: Savo, Merades Alco, benzalalkonium chloride. A persistent industry in isolate Listeria monocytogenes Lm-24 was used as the model organism for these tests. Benzalalkonium chloride treatment was found to be the most efficient way to damage the biofilm. One minute treatment with 500 mg/l was lethal for the biofilm cells, and that with 125 mg/l for planctonic cells. Savo suppresed the viability of the biofilm cells only by about 20% on average while being lethal for planctonic cells. Merades Alco exhibited only a weak effect on both the biofilm and planctonic cells.

Keywords: Listeria monocytogenes; biofilm; disinfectants

Published: August 31, 2010  Show citation

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PURKRTOVÁ S, TUROŇOVÁ H, PILCHOVÁ T, DEMNEROVÁ K, PAZLAROVÁ J. Resistance of Listeria monocytogenes biofilms to disinfectants 326. Czech J. Food Sci. 2010;28(4):326-332. doi: 10.17221/153/2010-CJFS.
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