Czech J. Food Sci., 2009, 27(11):SII66-SII74 | DOI: 10.17221/206/2009-CJFS

Characterisation of antibodies for the immunochemical detection of Enterobacter sakazakii

Igor Hochel1, Jiří Škvor2
1 Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic
2 Department of Medical Biophysics, 1 st Medical Faculty, Charles University in Prague, Prague, Czech Republic

An indirect competitive enzyme immunoassay of Enterobacter sakazakii has been developed. The rabbit polyclonal antibodies to heat-labile or heat-stable antigen of the type strain E. sakazakii CNCTC 5739T were prepared for these purposes. The detection limits of enzyme immunoassays were within the range 0.6-14.4 × 105 cells/ml. Antibodies raised to heat-labile antigen were serotype-specific. Although they contain non-specific IgG fractions binding periplasmatic and cytosol proteins, the interactions of these immunoglobulins are not manifested under conditions of ELISA developed.

Keywords: Enterobacter sakazakii; heat-labile antigen; IgG; ELISA; cross-reactivity

Published: December 31, 2009  Show citation

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Hochel I, Škvor J. Characterisation of antibodies for the immunochemical detection of Enterobacter sakazakii. Czech J. Food Sci. 2009;27(Special Issue 2):II66. doi: 10.17221/206/2009-CJFS.
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