Czech J. Food Sci., 2009, 27(11):SII50-SII55 | DOI: 10.17221/673-CJFS

Production of biogenic amines by Enterococci

Kateřina Kučerová, Hana Svobodová, ©těpán Tůma, Iva Ondráčková, Milada Plocková
Department of Dairy and Fat Technology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic

Enterococci were presented in all tested samples of raw cow milk (six samples) at the level 103-105 CFU/ml, fresh cheeses (five samples) at the level 102-106 CFU/g and semi-hard cheeses (five samples) at the level 103-105 CFU/g. All 33 isolated Enterococcus strains were screened for decarboxylase activity by usage differential growth medium and 20 of them possessed tyrosine decarboxylase activity. A collection of eight strains with the strongest decarboxylase activity were identified by species specific PCR as E. faecium (Z3, Z4, Br4 and 6/4D strains) and E. faecalis (®4, 3/3C and 4/1A strains). Enterococcus spp. Z1 strain was not identified at the species level by used methods, but the genus was confirmed by PCR method. Their tyrosin decarboxylase activity was confirmed by TLC and detection of tdc gene. Z1, Z3 and Z4 strains showed also histidine decarboxylase activity on the differential growth medium with histidine, but this activity was evaluated by TLC as a false positive reaction of medium.

Keywords: Enterococcus; decarboxylase; tyrosine; histidine; TLC; PCR

Published: December 31, 2009  Show citation

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Kučerová K, Svobodová H, Tůma ©, Ondráčková I, Plocková M. Production of biogenic amines by Enterococci. Czech J. Food Sci. 2009;27(Special Issue 2):II50. doi: 10.17221/673-CJFS.
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