Czech J. Food Sci., 2009, 27(11):SII12-SII17 | DOI: 10.17221/676-CJFS

Influence of Enterococci and Lactobacilli on Listeria

Kateřina Kučerová, Ivana Korbová, ©árka HORÁČKOVÁ, Eva ©viráková, Milada Plocková
Department of Dairy and Fat Technology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic

A collection of lactic acid bacteria (38 Enterococcus and 41 Lactobacillus strains) was tested for the antilisterial activity against 15 Listeria spp. strains (two L. monocytogenes, one L. ivanovii and 12 L. innocua strains) using agar spot method. Out of all 79 bacteria only six Enterococcus strains (1/3A, 3/3A, 6/4D, 6/1A, 1282 and EN3) exhibited antilisterial activity against almost all used indicator strains, when their live cells were tested. When their cell free neutralised supernatants (CFNS) were tested against four selected indicator strains (L. innocua Ln-03, Ln-06, Ln-10 and L. monocytogenes CCM5576) only two Enterococcus spp. strains were active - E. faecalis 6/1A strain from raw cow milk of minor interest due to the activity of its CFNS only against L. innocua Ln-06 and thermolability of the compound and E. mundtii 1282 strain from goat raw milk with CFNS active against 13 Listeria spp. strains including L. monocytogenes. E. mundtii 1282 strain produced probably a bacteriocin, because it completely lost the activity after treatment CFNS with proteinase K.

Keywords: Enterococcus; Lactobacillus; Listeria; antilisterial activity

Published: December 31, 2009  Show citation

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Kučerová K, Korbová I, HORÁČKOVÁ ©, ©viráková E, Plocková M. Influence of Enterococci and Lactobacilli on Listeria. Czech J. Food Sci. 2009;27(Special Issue 2):II12. doi: 10.17221/676-CJFS.
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    References

    1. Abriouel H., Lucas R., Ben Omar N., Valdivia E., Maqueda M., Martínez-Cañamero M., Gálvez A. (2005): Enterocin AS-48RJ: a variant of enterocin AS-48 chromosomally encoded by Enterococcus faecium RJ16 isolated from food. Systematic and Applied Microbiology, 28: 383-397. Go to original source... Go to PubMed...
    2. Arihara K., Cassens R.G., Luchansky J.B. (1993): Characterization of bacteriocins from Enterococus faecium with activity against Listeria monocytogenes. International Journal of Food Microbiology, 19: 123-134. Go to original source... Go to PubMed...
    3. Casaus P., Nilsen T., Cintas L.M., Nes I.F., Hernández P.E., Holo H. (1997): Enterocin B, a new bacteriocin from Enterococcus faecium T136 which can act synergistically with enterocin A. Microbiology, 143: 2287-2294. Go to original source... Go to PubMed...
    4. Chen H., Hoover D.G. (2003): Bacteriocins and their food applications. Comprehensive Reviews in Food Science and Food Safety, 2: 82-100. Go to original source... Go to PubMed...
    5. Cintas M.L., Casaus P., Håvarstein L.S., Hernández P.E., Nes I.F. (1997): Biochemical and genetic characterization of enterocin P, a novel sec-dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum. Applied and Environmental Microbiology, 63: 4321-4330. Go to original source... Go to PubMed...
    6. Cintas L.M., Casaus P., Herranz C., Håvarstein L.S., Holo H., Hernández P.E., Nes I.F. (2000): Biochemical and genetic evidence that Enterococcus faecium L50 produces enterocins L50A and L50B, the sec - dependent enterocin P, and a novel bacteriocin secreted without an N - terminal extension termed enterocin Q. Journal of Bacteriology, 182: 6806-6814. Go to original source... Go to PubMed...
    7. Del Campo R., Tenorio C., Jiménez-Díaz R., Rubio C., Gomez-Lus R., Baquero F., Torres C. (2001): Bacteriocin production in vancomycin - resistant and vancomycin - susceptible Enterococcus isolates of different origins. Antimicrobial Agents and Chemotherapy, 45: 905-912. Go to original source... Go to PubMed...
    8. Dussurget O. (2008): New insights into determinants of Listeria monocytogenes virulence. International Review of Cell and Molecular Biology, 270: 1-38. Go to original source... Go to PubMed...
    9. Eguchi T., Kaminaka K., Shima J., Kawamoto S., Mori K., Choi S.-H., Doi K., Ohmomo S., Ogata S. (2001): Isolation and characterization of enterocin SE-K4 produced by thermophilic enterococci, Enterococcus faecalis K-4. Bioscience, Biotechnology and Biochemistry, 65: 247-253. Go to original source... Go to PubMed...
    10. Franz C.M.P.A., Schillinger U., Holzapfel W.H. (1996): Production and characterization of enterocin 900, a bacteriocin produced by Enterococcus faecium BFE 900 from black olives. International Journal of Food Microbiology, 29: 255-270. Go to original source... Go to PubMed...
    11. Gálvez A., Valdivia E., Abriouel H., Camafeita E., Mendez E., Martínez-Bueno M., Maqueda M. (1998): Isolation and characterization of enterocin EJ97, a bacteriocin produced by Enterococcus faecalis EJ97. Archives of Microbiology, 171: 59-65. Go to original source... Go to PubMed...
    12. Gaya P., Sanchez J., Medina M., Nuñez M. (1998): Incidence of Listeria monocytogenes and other Listeria species in raw milk produced in Spain. Food Microbiology, 15: 551-555. Go to original source...
    13. S2-16
    14. Ghalfi H., Kouakou P., Duroy M., Daoudi A., Benkerroum N., Thonart P. (2006): Antilisterial bacteriocin-producing strain of Lactobacillus curvatus CWBI-B28 as a preservative culture in bacon meat and influence of fat and nitrites on bacteriocins production and activity. Food Science and Technology International, 12: 325-333. Go to original source...
    15. Giesová M., Chumchalová J., Plocková M. (2004): Effect of food preservatives on the inhibitory activity of acidoin CH5 and bacteriocin D10. European Food Research and Technology, 218: 194-197. Go to original source...
    16. Holck A., Axelsson L., Birkeland S.E., Aukrust T., Blom H. (1992): Purification and amino acid sequence of sakacin A, a bacteriocin from Lactobacillus sakei Lb706. Journal of General Microbiology, 138: 2715-2720. Go to original source... Go to PubMed...
    17. Hudáček J., Zsolt Z., Chumchalová J., Halász A. (2007): Antifungálny účinok laktobacilov na plesne rodu Fusarium a Aspergillus. Chemické listy, 101: 730-737.
    18. Hünke K., Axelsson L., Holck A., Kröckel L. (1996): Analysis of the sakacin P gene cluster from Lactobacillus sakei Lb674 and its expression in sakacin-negative Lb. sakei strains. Microbiology, 142: 1437-1448. Go to original source... Go to PubMed...
    19. Izquierdo E., Marchioni E., Aoude-Werner D., Hasselmann C., Ennahar S. (2009): Smearing of soft cheese with Enterococcus faecium WHE 81, a multibacteriocin producer, against Listeria monocytogenes. Food Microbiology, 26: 16-20. Go to original source... Go to PubMed...
    20. Kang J.H., Lee M.S. (2005): Characterization of a bacteriocin produced by Enterococcus faecium GM-1 isolated from an infant. Journal of Applied Microbiology, 98: 1169-1178. Go to original source... Go to PubMed...
    21. Larsen A.G, Norrung B. (1993): Inhibition of Listeria monocytogenes by bavaricin A, a bacteriocin produced by Lactobacillus bavaricus MI401. Letters in Applied Microbiology, 17: 132-134. Go to original source...
    22. Mareková M., Lauková A., DeVuyst L., Skaugen M., Nes I.F. (2003): Partial characterization of bacteriocins produced by environmental strain Enterococcus faecium EK13. Journal of Applied Microbiology, 94: 523-530. Go to original source... Go to PubMed...
    23. Martinez R.C.R., De Martinis E.C.P. (2005): Antilisterial activity of a crude preparation of Lactobacillus sakei 1 bacteriocin and its lack of influence on Listeria monocytogenes haemolytic activity. Food Control, 16: 429-433. Go to original source...
    24. Moreno M.R.F., Sarantinopoulos P., Tsakalidou E., Vuyst L.D. (2006): The role and application of enterococci in food and health. International Journal of Food Microbiology, 106: 1-24. Go to original source... Go to PubMed...
    25. Ouwehand A.C. (1993): Antimicrobial components form lactic acid bacteria. Marcel Dekker, Inc., New York: 139-159.
    26. Plocková M., Chumchalová J., Giesová J., Bouąková O. (2004): Antifungal effectiveness of Lactobacillus rhamnosus VT1 at different temperatures. Advances in Food Sciences, 26: 64-68.
    27. Rudolf M., Scherer S. (2001): High incidence of Listeria monocytogenes in European red smear cheese. International Journal of Food Microbiology, 63: 91-98. Go to original source... Go to PubMed...
    28. Sabia C., Manicardi G., Messi P., de Niederhäusern S., Bondi M. (2002): Enterocin 416K1, an antilisterial bacteriocin produced by Enterococcus casseliflavus IM 416K1 isolated from Italian sausages. International Journal of Food Microbiology, 75: 163-170. Go to original source... Go to PubMed...
    29. Schillinger U., Stiles M.E., Holzapfel W.H. (1993): Bacteriocin production by Carnobacterium pisciola LV 61. International Journal of Food Microbiology, 20: 131-147. Go to original source... Go to PubMed...
    30. Simon L., Fremaux C., Cenatiempo Y., Berjeaud J.M. (2002): Sakacin G, a new type of antilisterial bacteriocin. Applied and Environmental Microbiology, 68: 6416-6420. Go to original source... Go to PubMed...
    31. Tichaczek P.S., Vogel R.F., Hammes W.P. (1993): Cloning and sequencing of curA encoding curvacin A, the bacteriocin produced by Lactobacillus curvatus LTH1174. Archives of Microbiology, 160: 279-283. Go to original source... Go to PubMed...
    32. Tůma ©., Vogensen F.K., Plocková M., Chumchalová J. (2007): Isolation of antifungally active lactobacilli from edam cheese. Acta Alimentaria, 36: 405-414. Go to original source...
    33. Tůma ©., Kučerová K., Plocková M. (2008): Isolation of anticlostridially active lactobacilli from semi-hard cheese. Czech Journal of Food Sciences, 26: 324-332. Go to original source...
    34. Zhou K., Zhou W., Li P., Liu G., Zhang J., Dai Y. (2008): Mode of action of pentocin 31-1: an antilisterial bacteriocin produced by Lactobacillus pentosus from Chinese traditional ham. Food Control, 19: 817-822. Go to original source...

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