Czech J. Food Sci., 2009, 27(6):454-462 | DOI: 10.17221/138/2008-CJFS

Sanitation of fresh green asparagus and green onions inoculated with Salmonella

Miguel A. Martínez-Téllez1, Francisco J. Rodríguez-Leyva3, Ilce E. Espinoza-Medina3, Irasema Vargas-Arispuro2, Alfonso A. Gardea1, Gustavo A. González-Aguilar1, J. Fernando Ayala-Zavala1
1 Coordinación de Tecnología de Alimentos de Origen Vegetal
2 Coordinación de Ciencias de los Alimentos. Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo, Sonora, México
3 Unión Agrícola Regional de Productores de Hortalizas y Frutas de San Luís Río Colorado, Carretera a Riíto, Colonia La Mesa, San Luís Río Colorado, Sonora, México

The absence of good agricultural and manufacturing practices in the production and postharvest handling of fresh produce, such as green asparagus or green onions increase the contamination risk by biological hazards like Salmonella. The objective of this work was to investigate the efficacy of chlorine (200 and 250 ppm), hydrogen peroxide (1.5% and 2%), and lactic acid (1.5% and 2%) sanitisers during different exposure times (40, 60, and 90 s) on the reduction of Salmonella enterica subspecie enterica serovar Typhimurium in inoculated fresh green asparagus and green onions. Washing with clean water only reduced < 1 log10 CFU/g in both vegetables. The most effective sanitiser evaluated for fresh green asparagus and green onions disinfection appeared to be 2% lactic acid reducing Salmonella growth close to 3 log10 CFU/g. Hydrogen peroxide was the least effective agent for Salmonella Typhimurium reduction. No effect was observed of the exposure time of inoculated product to sanitiser up to 90 seconds. These results confirm that lactic acid could be used as an alternative for fresh green asparagus and green onions sanitation.

Keywords: green asparagus; green onions; lactic acid; hydrogen peroxide; sodium hypochlorite; Salmonella Typhimurium

Published: December 31, 2009  Show citation

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Martínez-Téllez MA, Rodríguez-Leyva FJ, Espinoza-Medina IE, Vargas-Arispuro I, Gardea AA, González-Aguilar GA, Ayala-Zavala JF. Sanitation of fresh green asparagus and green onions inoculated with Salmonella. Czech J. Food Sci. 2009;27(6):454-462. doi: 10.17221/138/2008-CJFS.
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    References

    1. Azanza M.P.V. (2004): Hydrogen peroxide, peroxyacetic acid, octanoic acid, peroxyoctanoic acid, and 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP) as components of antimicrobial washing solution. Chemical and Technical Assessment, 63: 1-7.
    2. Beuchat L.R. (1995): Pathogenic microorganism associated with fresh produce. Journal of Food Protection, 59: 204-216. Go to original source... Go to PubMed...
    3. Beuchat L.R., Nail B.V., Adler B.B., Clavero M.R.S. (1998): Efficacy of spray application of chlorinated water in killing pathogenic bacteria on raw apples, tomatoes, and lettuce. Journal of Food Protection, 61: 1305-1311. Go to original source... Go to PubMed...
    4. Beuchat L.R., Ryu J.H. (1997): Produce handling and processing practices. Emerging Infectious Diseases, 3: 459-465. Go to original source... Go to PubMed...
    5. Brackett R.E. (1992): Shelf stability and safety of fresh produce as influenced by sanitation and disinfection. Journal of Food Protection, 55: 804-814. Go to original source... Go to PubMed...
    6. Calvin L. (2003): Produce, Food Safety, and International Trade. Response to U.S. Foodborne Illness Outbreaks Associated with Imported Produce. In: Buzby J. (ed.): International Trade and Food Safety: Economic Theory and Case Studies. USDA-Economic Research Service, Washington.
    7. CDC (2003): Hepatitis A outbreaks associated with green onion at a restaurant - Monaca Pennsylvania. Morbidity and Mortality Weekly Report, 47: 1155-1157
    8. CDC (2008): Outbreak of Salmonella serotype Saintpaul infections associated with multiple raw produce items Report, 57: 929-934.
    9. Cords B.R., Dychdala G.R. (1993): Sanitizers: halogens, surface-active agents and peroxides. In: Davidson P.M., Branen A.L. (eds): Antimicrobials in Foods. Marcel Dekker, New York: 469-537.
    10. Cuite C.L., Schefske S.D., Randolph E.M., Hooker N.H., Nucci M.L., Hallman W.K. (2009): Public response to the Salmonella Saintpaul outbreak of 2008. the State University of New Jersey, Food Policy Institute, New Brunswick, NJ, Rutgers.
    11. Cummings K., Barrett E., Mohle-Boetani J.C., Brooks J.T., Farrar J., Hunt T., Fiore A., Komatsu K., Werner S.B., Slutsker L. (2001): A multistate outbreak of Salmonella enterica serotype Baildon associated with domestic raw tomatoes. Emerging Infectious Diseases, 7: 1046-1048. Go to original source... Go to PubMed...
    12. Chin C.K., Garrison S.A. (2008): Functional elements from asparagus for human health. Acta Horticola (ISHS), 776: 219-226. Go to original source...
    13. D'Aoust J.Y. (1997): Salmonella species. In: Doyle M.P., Beuchat L.R., Montville T.J. (eds): Food Microbiology Fundamentals and Frontiers. ASM Press, Washington: 129-158.
    14. De-Roever C. (1998): Microbiological safety evaluations and recommendations on fresh produce. Food Control, 9: 321-347. Go to original source...
    15. Eswaranandam S., Hettiarachchy N.S., Meullenet J.F. (2006): Effect of malic and lactic acid incorporated soy protein coatings on the sensory attributes of whole apple and fresh-cut cantaloupe. Journal of Food Science, 71: S307-S313. Go to original source...
    16. FDA (1998): Guide to Minimize Microbial Food Safety Hazards for Fresh Fruits and Vegetables. Availabe at h ttp://www.foodsafety.gov/~dms/prodguid.html (accessed Janurary 27, 2007)
    17. Garg N., Churey J.J., Splittstoesser D.F. (1990): Effect of processing conditions on the microflora of fresh-cut vegetables. Journal of Food Protection, 53: 701-703. Go to original source... Go to PubMed...
    18. Greene S.K., Daly E.R., Talbot E.A., Demma L.J., Holzbauer S., Patel N.J., Hill T.A., Walderhaug M.O., Hoekstra R.M., Lynch M.F., Painter J.A. (2008): Recurrent multistate outbreak of Salmonella Newport associated with tomatoes from contaminated fields, 2005. Epidemiology and Infection, 136: 157-165. Go to original source... Go to PubMed...
    19. Hintze J. (2001): NCSS and PASS. Number Cruncher Statistical Systems. Kaysville, Utah, USA.
    20. Hsing A.W., Chokkalingam A.P., Gao Y.T., Madigan M.P., Deng J., Gridley G., Fraumeni J.F. (2002): Allium vegetables and risk of prostate cancer: a population-based study. Journal of the National Cancer Institute, 94: 1648-1651. Go to original source... Go to PubMed...
    21. Ibarra-Sánchez L.S., Alvarado-Casillas S., Rodríguez-García M.O., Martínez-Gonzáles N.E.A.C. (2004): Internalization of bacterial pathogens in tomatoes and their control by selected chemicals. Journal of Food Protection, 67: 1353-1358. Go to original source... Go to PubMed...
    22. Martínez-Téllez M.A., Vargas-Arispuro I., Cuamea-Navarro F., Morón C. (2005): Producción primaria y Manejo. Handling and production: Nuevas Tecnologías de Conservación: Productos Vegetales Frescos Cortados. In: González-Aguilar G.A., Gardea A.A., Cuamea-Navarro F. (eds): Emerging Technologies for Preservation: Fresh-Cut Fruit and Vegetables. Hermosillo, Sonora, Mexico, Logiprint.
    23. Park C.M., Beuchat L.R. (1999): Evaluation of sanitizers for killing Escherichia coli O157:H7, Salmonella and naturally occurring microorganisms on cantaloupes, honeydew melons, and asparagus. Dairy Food and Environmental Sanitation, 19: 824-847.
    24. Parnell T.L., Harris L.J. (2003): Reducing Salmonella on apples with wash practices commonly used by consumers. Journal of Food Protection, 66: 741-747. Go to original source... Go to PubMed...
    25. Rodriguez-Leyva F.J. (2004): Determinación de las fuentes de contaminación bacteriológicas y tratamientos de desinfección en las cadenas productivas de cebollin (Allium fistolum) y esparrago (Asparagus officinalis). [Thesis: Master in Science.] Coordinación de Tecnología de Alimentos de Origen Vegetal, Centro de Investigación en Alimentación y Desarrollo, A.C.: 57.
    26. Sanz S., Jiménez M., Olarte C., Lomas C., Portu J. (2002): Effectiveness of chlorine washing disinfection and effects on the appearance of artichoke and borage. Journal of Applied Microbiology, 93: 986-993. Go to original source... Go to PubMed...
    27. Sapers G.M., Miller R.L., Annous B.A., Burke A.M. (2002): Improved antimicrobial wash treatments for decontamination of apples. Journal of Food Science, 67: 1886-1891. Go to original source...
    28. Sapers G.M., Miller R.L., Pilizota V., Mattrazo A.M. (2001): Antimicrobial treatments for minimally processed cantaloupe melon. Journal of Food Science, 66: 345-349. Go to original source...
    29. Singh N., Singh R.K., Bhunia A.K., Stroshine R.L. (2002): Effect of inoculation and washing methods on the efficacy of different sanitizers against Escherichia coli O157:H7 on lettuce. Food Microbiology, 19: 183-193. Go to original source...
    30. Taormina P.J., Beuchat L.R. (1999): Behavior of enterohemorrhagic Escherichia coli O157:H7 on alfalfa sprouts during the sprouting process as influenced by treatments with various chemicals. Journal of Food Protection, 62: 850-856. Go to original source... Go to PubMed...
    31. Thunberg R.L., Tran T.T., Bennett R.W., Matthews R.N., Belay N. (2002): Microbial evaluation on selected fresh produce obtained at retail markets. Journal of Food Protection, 65: 677-682. Go to original source... Go to PubMed...
    32. Torriani S., Orsi C., Vescovo M. (1997): Potential of Lactobacillus casei, culture permeate, and lactic acid to control microorganisms in ready-to-use vegetables. Journal of Food Protection, 60: 1564-1567. Go to original source... Go to PubMed...
    33. Ukuku D.O., Fett W.F. (2002): Effectiveness of chlorine and nisin-EDTA treatments of whole melons and fresh-cut pieces for reducing native microflora and extending shelf-life. Journal of Food Safety, 22: 231-257. Go to original source...
    34. Venkitanarayanan K.S., Lin C.M., Bailey H., Doyle M.P. (2002): Inactivation of Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes on apples, oranges and tomatoes by lactic acid with hydrogen peroxide. Journal of Food Protection, 65: 100-105. Go to original source... Go to PubMed...
    35. Wisniewski M.A., Glatz B.A., Gleason M.L., Reitmeier C.A. (2000): Reduction of Escherichia coli O157: H7 counts on whole fresh apples by treatment with sanitizers. Journal of Food Protection, 63: 703-708. Go to original source... Go to PubMed...
    36. Yu K., Newman M.C., Archbold D.D., HamiltonKemp T.R. (2001): Survival of Escherichia coli O157: H7 on strawberry fruit and reduction of the pathogen population by chemical agents. Journal of Food Protection, 64: 1334-1340. Go to original source... Go to PubMed...

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