Czech J. Food Sci., 2009, 27(5):386-391 | DOI: 10.17221/234/2008-CJFS

SE-HPLC separation of myosin complex with tannins from bearberries (Arctostaphylos uva-ursi L. Sprengel) leaves - a short report.

Ryszard Amarowicz1, Ronald B. Pegg2, Agnieszka Kosińska1
1 Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Division of Food Science, Olsztyn, Poland
2 Department of Food Science and Technology, University of Georgia, Athens, USA

Phenolic compounds were extracted from bearberry (Arctostaphylos uva-ursi L. Sprengel) leaves into 95% (v/v) ethanol. The tannin constituents were separated from the crude extract using Sephadex LH-20 column chromatography with 95% (v/v) ethanol and 50% (v/v) acetone as the mobile phases. Myosin was isolated and purified from excised pork knuckle muscles using the standard salt-solution extraction procedure followed by gel filtration chromatography. Myosin was precipitated from the solution with bearberry-leaf tannins at pH 5.0. The recovered complex was washed, lyophilised, and subjected to size-exclusion high performance liquid chromatography (SE-HPLC). Based on the basic conditions of the HPLC analysis, a portion of the tannin constituents was liberated from the complex. The UV spectra of these compounds were characterized by a maximum at ~ 300 nm. A portion of the tannins was present in the complex with myosin, and this was confirmed by UV spectra.

Keywords: myosin; tannins; SE-HPLC; bearberry; protein-tannins complex

Published: October 31, 2009  Show citation

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Amarowicz R, Pegg RB, Kosińska A. SE-HPLC separation of myosin complex with tannins from bearberries (Arctostaphylos uva-ursi L. Sprengel) leaves - a short report. Czech J. Food Sci. 2009;27(5):386-391. doi: 10.17221/234/2008-CJFS.
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